Serologic study on leptospiral infection in sheep in Ahvaz, southwestern Iran
Message:
Abstract:
To investigate the seroprevalence of leptospiral infection in sheep in Ahvaz, southwestern Iran, blood samples were taken from 181 female sheep. Sera were stored at -20°C until use. They were initially screened at serum dilution of 1:100 against six live antigens of Leptospira interrogans serovars pomona, canicola, hardjo, ballum, icterohaemorrhagiae, grippotyphosa using the microscopic agglutination test (MAT). The samples were considered positive if > 50% of agglutination of leptospire in a dilution test serum of > l:100 were observed. Sera with positive results were titrated against reacting antigens in serial two-fold dilutions from l:100 to 1:1600. Antibodies against one or more serovars were detected in 27 (14.9%) sera at dilution ³1:100. Antibodies against more than one serovar were found in 5 (18.5%) positive sera. Among the positive sera, antibodies were most frequent to serovar pomona (43.8%) followed by canicola (21.9%), icterohaemorrhagiae (12.5%), grippotyphosa (9.4%), ballum and hardjo (each of them 6.3%). The results of this survey indicate that leptospiral infection is common in sheep in Ahvaz and that various serovars concur in the etiology.
Language:
Persian
Published:
Iranian Journal of Veterinary Research, Volume:8 Issue:4, 2008
Page:
333
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