Extraction and identification of β-stigmasterol and two flavonoids from the aerial parts of Tanacetum canescens (DC.)

The genus of Tanacetum belongs to Compositeae family, distributed in South and Southwestern Europe, Southwestern of Asia and North of Africa. The aerial parts of Tanacetum Canescens (DC.) were collected during the flowering stage from Sanandaj in Kordestan Province. The chloroform extract of T. canescens was investigated and β-stigmasterol and two flavonoids, 5-hydroxy 6,7, 4'- trimethoxy flavone and 5,3'- dihydroxy 6,7, 4'– trimethoxy flavone were isolated. The structure of these natural products was elucidated by using 1H and 13C Nuclear Magnetic Resonance (NMR) spectroscopy.