Effect of Pretreatment of Salicylic acid on Wheat (Triticum aestivum L.) Seed Germination under Salt Stress

The effects of pretreatment of Salicylic acid on wheat seed germination (Triticum aestivum L. cvs. Roshan), lipids peroxidation, superoxide dismutase (SOD), catalase (CAT), polyphenol oxidase (PPO) peroxidase (POX) activity in salt stress condition was investigated. Treatments of wheat seedlings with different concentrations of salicylic acid (SA) for seedling growth (0, 0.5, 1mM) have been used. Salt stress was inducted by NaCl solution (0, 50 100, 200 mM). For this purpose after surface sterilization of seeds, they were soaked in SA solutions for 24 h and then dried by sterile paper then transferred in to sterile petri dishes and added 10 ml NaCl solution with different concentrations. After one week number of germinated seed, radical length, seedling length and dry weight were recorded. Antioxidant enzymes activity and lipid peroxidation were assayed too. The results showed salinity decreased seed germination. Thus, high concentration of NaCl (200mM) decreased germination 17/6% than control treatment. Influence of SA was significant and increased percent of germination in stressed and control treatments. SA increased the level of cell division of seedlings and roots which caused an increase in plant growth. Enzymes assay showed, enzyme activity was increased in salt stress conditions and SA reduced activity of antioxidant enzyme. In brief, the SA treatment reduced the damaging action of salinity on seedling growth and accelerated a restoration of growth processes.