Isolation and detection of Mycoplasma synoviae from seropositive rapid reaction broiler breeder flocks by polymerase chain reaction and culture methods

Mycoplasma synoviae (MS) is one of the main disease in fowl which cause infections of respiratory system and tenosynovitis. Characterization and successful detection ofMS in broiler breeder farms help to prevent distribution of MS in broiler farms.The aim of this study was isolation and characterization of MS in broiler breeder farms by cultureand polymerase chain reaction (PCR) techniques. Thirty specimens which obtained from flocks with positive serum reactions were studied by culture and PCR techniques.After collecting of Choanal cleft and tracheal swabs in PPLO broth, a short-term incubation was occurred and then filtered and a serial passage was performed. In thefourth passage, all of the specimens cultured on PPLO agar, in addition, original specimens used to extract of DNA and MS-PCR technique. Results showed that 15/30were positive by culture (Approved by using of standard MS specific antiserum) and 25/30 were positive by PCR. These observations highlight the higher sensitivity of PCRrather than culture. These results may be observed because of PCR ability to detect DNA after mycoplasma´s death.