Scanning Electron Microscopy study of morphology of totudent and bio-oss bone grafts on SaOS-2 cells

Several reports have been published on clinical application of various Bone Substitute Material (BSM) which show their successful application. The most important factor in determination of BSM success is the physiological and histological behavior of this material that is evaluated by morphology of osteogenic cells, rate of survival and their proliferation. This research was done to assess the effect of this material on morphology, proliferation and rate of survival of SaOS-2 osteoblastoid cells. In this experimental in vitro study, two types of materials of bone graft (totudent and bio-oss) were assessed for their effect on survival rate, proliferation and morphology of SaOS-2 cells. 12 samples from each of grafted material and 4 negative control samples have been used. In day 21, morphology of these cells was evaluated by scanning electron microscopy (SEM). In the term of spiral morphology, there was the meaningful statistics difference between Cerasorb and two other groups, but there was not meaningful difference between two groups of totudent and control. In the term of spindle morphology, there was meaningful statistics difference between the above mentioned groups. In the term of elongated morphology with pseudopodia, none of them had meaningful statistics difference. ‍ The synthetic material of totudent bone graft compared with bio-oss, showed better result in the morphologic variations of SaOS-2 cells at culture medium and has better biologic characteristic.