Effect of Cultivar and Density of Cultured Cotyledons on Shoot Regeneration in Rapeseed (Brassica napus L.)

The objective of the present research was to study the effect of genotypes (2 commercial cultivars of repeseed, PF-7045-91 and SLM-046) and explant densities (15 and 30 cotyledons in 10 cm in diometer Petri dishes) on shoot regeneration. Results showed that there were significant differences between rapeseed cultivars and explant densities, but no significant interaction effect was observed between investigated parameters. The cultivar PF-7045-91 was better regenerated with average of 85% regenerated cotyledons than the other one with 62%. Higher percentage of regeneration (87%) was also recorded for treatment with 15 cotyledons in Petri dish rather than 30 cotyledons.