Frequency of Ompk35 And Ompk36 in Strains of

Klebsiella pneumonia is a major nosocomial pathogen causing pneumonia، urinary tract infections، and bacteremia، particularly in immune-compromised patients. The aim of our study was expression of OMPK36 and OMPK35 in ESBLs production and non-ESBLs K. pneumoniae. Clinical isolates of K. pneumoniae were identified. ESBLs were assayed by Phenotypic (screening and confirming) and genotypic (PCR) methods. OMPK35 and OMPK36 were tested by polymerase chain reaction and SDS- PAGE. Our findings showed that 42. 3% of K. pneumoniae were ESBLs positive. Twenty-two K. pneumoniae producing ESBLS and Twenty-two non-ESBLs K. pneumoniae were selected for porines detection. We found out that 54. 54% and 72. 72% of K. pneumoniae producing ESBLS were positive for Ompk35 and Ompk36 respectively، while our findings in non-ESBLs K. pneumoniae showed that 95. 94% and 100% of isolates were positive for Ompk35 and Ompk36، respectively. Discussion & Many of clinical isolates of non- ESBL K. pneumoniae had expressed both Ompk35 and Ompk36 porins، while our results in ESBLs positive K. pneumoniae had only showed expression of Ompk36. A few of non-ESBLs and ESBLs K. pneumoniae were found as negative for both OMPK35 and OMPK36. Our findings released that lack of porins in K. pneumoniae are defined as an antibiotic- resistant mechanism in many isolates.