Comparison of the effects of growth factors on mouse embryo preimplantation development resulted vitrification

The aim of this study is to improve development outcome of vitrified mouse embryos by adding of fibroblast growth factor (FGF) and Hepatocyte growth factor (HGF) into medium culture. Two-cell mouse embryos were obtained from oviduct and then vitrified by cryotop. After warming the embryos, they were cultured in T6 medium supplemented with dose of 20 ng/ml of each FGF and HGF. Embryo developmental rate was compared between vitrified, non vitrified and control groups. Embryos development rate was assessed. The results showed that cleavage and development rate of vitrified and non-vitrified mouse embryos increased significantly in the culture medium supplemented with growth factors as compared to the control group (p<0.01). Also, there is significant difference in morula developmental rates between vitrified and non-vitrified embryos treated with growth factors (p<0.05), but this difference has not been observed in blastocyst formation rate (p>0.05). The results of this study demonstrate that enriching the culture medium with growth factors improves preimplantation developmental rates of mouse embryos after vitrification