Assay of enzyme production in isolated bacteria from Seabream, Acanthopagrus latus, in in vitro condition with the purpose of probiotic screening

This study was carried out to investigate the specific activity of amylase, protease and lipase, as a selective criterion of probiotics, in some Bacillus sp. and Lactobacillus sp. isolated from intestinal tract of yellowfin seabream, Acanthopagrus latus. The results showed that all seven Bacillus and three Lactobacillus isolates were able to produce enzymes, although the specific enzyme activity was significantly different between the isolates. The specific activity of enzymes was higher in Bacillus isolates than in Lactobacillus isolates. Maximum and minimum activity of protease was observed in Bacillus BP5 isolates (3.13 IU/mg protein) and Lactobacillus LP1 isolate (1.03 IU/mg protein) respectively (P<0.05). Highest and lowest amylase activity was observed in Bacillus BP6 (3.00 IU/mg protein) and Lactobacillus LP2 (0.04IU/mg protein) respectively. Bacillus BP6 (1.40 IU/mg protein) had maximum lipase activity and was significantly different from other isolates (P<0.05).