Application of Microsatellite Markers to Assess Hybridization between two Species of Commercial Salmonids fish of Iran

Hybridization is one of the traditional breeding programs in aquaculture. When a new hybrid produced، it is essential to evaluate its characteristics. Different characteristics like morphometric and meristic، physiological، karyological and molecular were used to detect hybrids. Molecular methods like microsatellite is one of the most important and pioneering tools for hybrid detection. In this research، the possibility of microsatellite markers to evaluate hybridization between two important salmonid fish، female rainbow trout Oncorhynchus mykiss and the male Caspian salmon Salmo trutta caspius was tested. Microsatellite primers، Str151INRA، Str73INRA، Str85INRA، Str543INRA، Str591INRA، Strutta58، Strutta12، Ssa197، Ssa171 and OmyFgt1TUF were selected and parents genotype in single pair mating manner (for 3 pairs of fish) were analyzed through PCR. Ssa197 and Ssa171 did not have any junction site on the genome. The others (except OmyFgt1TUF) did not show polymorphism between brood fish and/or produce at least one common band between male and female، so it was impossible to use them as marker for hybrid detection. OmyFgt1TUF showed remarkable polymorphism and clear specific bands between brood fish; the progenies had complete heritage of specific bands of two parents confirming that crossing between female rainbow trout and the male Caspian salmon produce real hybrids. This research showed the possibility of using microsatellite markers to detect hybridization between two species of commercial Iranian salmonids.