مجله علوم و صنایع غذایی ایران
سال بیستم شماره 9 (پیاپی 142، آذر 1402)

Considering the high perishability, quality reduction, and nutritional value of the fish during the storage period, the aim study is to investigate the edible coating based on chicken feet gelatin/green walnut skin extract to preserve the physical characteristics, color, and texture properties of Oncorhynchus mykiss fillet during storage in the refrigerator. After extracting gelatin and green walnut skin extract, the coating solution were prepared. The fillets were coated by immersion method. Physical characteristics (moisture), color indices (L*, a*, and b*) as well as textural properties (hardness, springiness, and Cohesiveness) of the fillets were evaluated during 12 days of storage (on days 0, 4, 8, and 12). Variance analysis was performed by Minitab software (version 18). The mean data comparison by Tukey's test is at a 95% confidence level. The results showed significant moisture retention of coated fillets (p<0.05). The L* and a* index of fillets decreased during 12 days of storage. While, the b* index was increased in all samples (p<0.05). The evaluation of the texture of the fillets showed a decrease in hardness and Cohesiveness, but springiness was increased (p<0.05). The coat had significantly higher hardness, Cohesiveness, and lower springiness than the control sample (p<0.05). Increasing the concentration of green walnut skin extract from 500 to 1000 ppm increased the quality of fillets (p<0.05). In general, It can be concluded that edible coating based on chicken leg gelatin/green walnut skin extract effective to increase shelf- life aquaculture industry.

Today, the desire to use ozone as a strong antimicrobial compound, cost-effective, and eco-friendly innovation, has increased to increase the shelf life of food products. This study was conducted in order to evaluate the chemical and microbial properties of thermally pasteurized skim milk treated with pressurized ozone injection at concentrations of zero, 1.5, 5, and 10 ppm. The chemical, microbial and sensory characteristics of the treated milk samples were evaluated on days 0, 4, 7 and 15 of storage in the refrigerator. The total viable count (TVC) in the treated samples with ozone compared to the control had a noticeable decrease in all storage days. The highest antimicrobial effect was observed on the 15th day of storage with 10 ppm ozone and the lowest effect was observed on the 4th day with 1.5 ppm ozone. However, there was no significant difference in pH (6.8-6.88) and acidity (0.16-0.18) between treated and control samples. Although the peroxide number (PV) in the treated samples and the control samples was higher than the standard, but its reduction was evident in the treated samples compared to the control samples. Also, by comparing the sensory characteristics among the samples, no significant differences were observed. In general, treatments with 5 and 10 ppm ozone had the best efficiency in increasing the shelf life of pasteurized milk. According to the results, ozone can be used as an auxiliary method to increase the effectiveness of thermal processes in pasteurization and increase the shelf life of milk.

In this study, the effect of temperature and storage time on biological activity and physicochemical stability of nanovesicles (liposomes and niosomes) containing bee pollen hydrolyzed protein obtained from alcalase and pepsin enzymatic hydrolysis was evaluated. 0.2% chitosan was used to coat nanoliposomes. DPPH radical scavenging power, ferric ion reducing power, ACE scavenging power, particle size, particle dispersion index, zeta potential, encapsulation efficiency and release rate of hydrolyzed proteins from nanovesicles during 28 days storage at refrigerator and ambient temperature were investigated. Results of DLS showed that the size of nanovesicles increased significantly by loading with hydrolyzed protein and coated with chitosan (P<0.05). Chitosan coated nanoliposomes had the highest amount of PDI. The zeta potential of nanovesicles reached the highest value by coating with chitosan. Chitosan coated nanoliposomes had the highest encapsulation efficiency. After 28 days, the size of coated and uncoated nanovesicles increased 2-26 times.The encapsulation efficiency of nanonisomes and uncoated nanoliposomes showed the lowest and highest decrease, respectively. The values of the measured factors during storage at the refrigerator were significantly lower than ambient temperature (P<0.05). The decline in the antioxidant activities of nanovesicles was significantly prevented by loading hydrolyzed proteins and coating the nanovesicles with chitosan. The ACE inhibition was lower in the nanoliposomes as compared with the nanoniosomes. After 28 days, the ACE inhibition activity of the loaded in nanoliposomes without coating chitosan decreased slightly. These findings are of great importance for designing and developing nutritious foods containing hydrolyzed protein.

The aim of this research was to optimize the formulation of a functional drink based on sugarcane juice using the response surface method (RSM) and in the form of Box Benken design. The ingredients used in the formulation were lemon juice (0.3, 6), turmeric (0.6, 0.3) and mint extract (0, 250, 500) in percentage and their physicochemical properties (pH, acidity, soluble solids, turbidity, color, total phenolic compounds, antioxidant activity) and sensory characteristics were investigated. The results showed that increasing the amount of lemon juice increased acidity, turbidity, L index, b index, phenolic compounds, antioxidant capacity and overall acceptance, but decreased pH, soluble solids and a index. The increase of turmeric caused a slight increase in the amount of soluble solids, b index and total phenolic compounds, while it decreased the antioxidant property and overall acceptance of the samples. With the increase in the amount of mint extract, turbidity, a and b indices, phenolic compounds and overall acceptance increased, but the amount of soluble solids and L index decreased. Finally, the optimization results showed that the sugarcane juice drink containing lemon juice (6%), turmeric (0.2%) and mint extract (418%) had the best physicochemical properties and the highest overall acceptance and can be recommended as a healthy drink in the industry.

The objective of this study was to investigate the effect of replacing of rice flour with different ratios (0, 10, 20, and 20% w/w) of pumpkin powder on the rheology of batter and textural characteristics of gluten free rice cake. A factorial experiment with a completely randomized design with three replications was used for data analysis. The use of pumpkin powder in rice cake formulations significantly improved rheology of batter and textural characteristics of the samples (p< 0.05). By increasing the replacement level of rice flour with pumpkin powder to 30%, viscosity (from 682.66 to 3518.44 cp), hardness (from 21.11 to 53.97 g), cohesiveness (from 0.83 to 0.94), and springiness (from 9.74 to 13.49 mm) of batter cake improved significantly, but the adhesiveness (3.15 mJ) of batter increased compared to control (1.08 mJ) (p <0.05). However, the incorporation of pumpkin powder in the sample formulations up to 30%, increased the hardness (from 456.17 to 800.11 g), cohesiveness (from 0.56 to 0.67), springiness (from 8.68 to 11.43 mm), and chewiness (from 2231 to 6148 g) of the final cakes. Addition of pumpkin powder (up to 30%) in gluten free rice cake formulation caused the significant increasing of the total color differences (ΔE), browning index, and saturation index (chroma) in the cakes compared to control and moreover, increased the hue angle to 90owhich indicated an increase in the yellowness of the samples containing higher the replacement level of rice flour with pumpkin powder.

The presence of methicillin-resistant (MRSA) and vancomycin-resistant (VRSA) Staphylococcus aureus in food raises a public health concern. This study aimed to investigate the antibacterial and anti-biofilm activity of some Lamiaceae essential oils including Melissa Officinalis, Salvia officinalis, and Mentha piperita against MRSA and for the first time on VRSA strains. For this purpose, the disk diffusion test, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), minimum biofilm inhibitory concentration (MBIC), and minimum biofilm eradication concentration (MBEC) were determined. Then, M. Officinalis essential oil compounds were investigated against PBP2a, agrA, and Bap proteins using AutoDocK Vina. Finally, pharmacokinetic properties were investigated using ADMETsar and SwissADME servers. Based on the obtained results, the MIC and MBC values of M. Officinalis essential oil against MRSA strain were equal to 0.05 and 0.112 mg/ml, and against VRSA strain were equal to 1.8 and 2.5 mg/ml, respectively. The MBIC and MBEC of M. Officinalis essential oil against MRSA strain were equal to 0.03 mg/ml and 0.112 mg/ml, and against VRSA strain were equal to 0.9 mg/ml and 3.2 mg/ml, respectively. The results of molecular docking showed that β-Caryophyllene had a greater binding affinity to PBP2a protein either in the active site or in the allosteric site (-6.6kcal/mol). On the other hand, the effective compounds of this essential oil, especially citronellol, thymol, and citral, were acceptable in terms of pharmacokinetic properties. Since natural antibiotics can be an alternative to conventional antibiotics in the treatment of Staphylococcus aureus food-borne diseases, the results of this study showed that Melissa Officinalis essential oil is effective on the growth and biofilm of MRSA and VRSA strains, and it can be used as a drug candidate in the prevention and treatment of infections caused by antibiotic-resistant strains of this bacterium.

In this study, an optimal pH-sensitive intelligent film based on starch at the level of 1% w/v containing 0.1% potato starch nanocrystals and anthocyanin extracted from the Dutch rose plant (Rosa hybrida) at the level of 0.5% w/v was designed and used to determine the spoilage of chicken fillet at refrigerator temperature during 12 days of storage. The index of volatile nitrogen bases ¹ and thiobarbituric acid ² of chicken samples increased significantly during 12 days.
The results showed that the inoculation of the extract in the biofilm changed the total population of bacteria and psychrophilic bacteria in chicken fillets to below detectable levels within 8 and 12 days, respectively. The present study showed that the color change of the smart film at the end of the storage day corresponds to the microbial growth pattern and also the increase in TBA, TVB-N and pH, which is caused by the production of nitrogenous substances and alkaline compounds by mesophilic and psychrophilic bacteria.
Therefore, the film of potato starch nanocrystals/potato starch containing Dutch rose extract can be used as an indicator of the freshness of meat fillet.

In this study, the effect of edible coating based on Lallemantia iberica seed mucilage (LISM) with different contents (0, 0.5, 1, 1.5 and 2% vol/vol) of Citrus paradise essential oil nanoemulsion (CPN) was investigated on the microbial, chemical, and sensory qualities of lamb slices during cold storage. The antimicrobial results showed that at the end of the storage period, the lowest number of total viable bacteria (6.26 log CFU/g), psychrotrophic count (3.28 log CFU/g), Escherichia coli (0.95 log CFU/g), Staphylococcus aureus (0.87 log CFU/g) and fungi (1.12 log CFU/g) were observed in LISM+2% CPN sample. The highest and lowest pH at the end of the storage period were related to uncoated samples (6.42) and LISM+2%CPN (5.65). The amount of peroxide number and thiobarbituric acid in the control and LISM+2%CPN samples were 11.60 meq O2/kg and 1.20 mg MDA/kg and 5.40 meq O2/kg and 0.59 mg MDA/kg, respectively, after 9 days of cold storage. The meat color (L*, a*, b*) was also preserved by edible coating containing C. paradise essential oil nanoemulsion (LISM+CPN). The addition of nanoemulsion in edible coating increased sensory scores such as aroma, color, texture, and overall acceptance of lamb slices, especially on the last day of cold storage. The obtained results suggest the LISM+CPN edible coating as a solution for retarding the chemical and microbial spoilage of lamb slices.

Notice to optimizing the use of medicinal plants by producing soft drinks can be a suitable solution in order to encourage consumers to use food with natural source that have beneficial effects on the health. The purpose of this research is optimizing the extraction process of Hibiscus Tea and providing the best way to preserve the nutritional value of this plant during extraction and applying this extract in diet soft drinks formulation by using the natural sweetener Stevia and evaluation of physico-chemical changes during storage. Thus, extracts of Hibiscus Tea was done in three temperatures of 60, 75 and 90 ° C in the range of 10 to 20 minutes and to select the best extraction temperature and time in Hibiscus Tea samples, terms of PH, antioxidant activity, anthocyanins and phenolic compounds were studied. Then by using extraction of Hibiscus Tea, soda with four different formulations was prepared. To consider the durability of soda, in 90 days, every 30 days lasting soft drink samples in terms of antioxidant activity, anthocyanins and phenolic compounds were evaluated. Finally it was determined that temperature 41.71 ° C for 18.81 minutes is the best temperature and time for extracting Hibiscus Tea, that In this optimal conditions, the antioxidant activity 62.4062%, the amount of anthocyanins 72.394 mg/l and total phenolic compounds 65.2564 mg/100 ml was determined. Also it was detrmined that antioxidant activity, anthocyanins and phenolic compounds at the end of storage time than the first day of manufacturing soft drinks decreased.

Datura stramonium is well known as a valuable drug in many diseases including inflammation, wounds, fever and toothache, and its extract has significant antimicrobial activity. Therefore, this study was performed to isolate the ethanolic extract of D. stramonium leaves and to investigate its antimicrobial activity. In this study, D. stramonium extract was obtained using the maceration method. Antimicrobial methods of disk diffusion agar, well diffusion agar, minimum inhibitory concentration, and minimum bactericidal concentration were used to determine the antibacterial activity of ethanolic extract of D. stramonium leaves against Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, and Bacillus cereus. The antimicrobial activity of the extract was concentration dependent and the highest diameter of growth inhibition zone was observed at a concentration of 60 mg/ml of the extract. According to disk diffusion agar and well diffusion agar tests, S. aureus and S. typhimurium had the highest and lowest diameter of growth inhibition zones, respectively. The minimum inhibitory concentrations for E. coli, S. typhimurium, S. aureus and B. cereus were 16, 16, 8 and 8 mg/ml, respectively, and the minimum bactericidal concentrations for these bacteria were 256, 256, 64, and 128 mg/ml, respectively. In general, Gram-positive bacteria were more sensitive to the extract than Gram-negative types. Ethanolic extract of D. stramonium leaves can be used as a natural antimicrobial agent to prevent the growth of pathogenic bacteria and treat related diseases.

In the present study, the hydroalcoholic extract of Withania somnifera was extracted and then the content of total phenol (by Folin-Ciocalteu reagent), total flavonoid (by aluminum chloride method), antioxidant activity (based on DPPH and ABTS free radical scavenging methods) and its antifungal effect against Rhizopu stolonifers, Botrytis cinerea, Penicillium expansum and Alternaria alternata (fungi cause spoilage postharvest apple and strawberry fruits) were determined based on disk diffusion agar, well diffusion agar, minimum inhibitory concentration, and minimum fungicidal concentration. The amount of phenol and flavonoids in the extract was 53.16 mg GAE/g and 28.20 mg QE/g, respectively. The antioxidant power of the hydroalcoholic extract was 69.80 and 57.15 μg/ml in terms of DPPH and ABTS free radical scavenging, respectively. The results of antimicrobial activity based on disk diffusion agar and well diffusion agar showed that increasing the concentration of the extract caused a significant increase in the diameter of the growth inhibition zone and Botrytis cinerea and Alternaria alternata were the most resistant and sensitive strains to the extract, respectively. In general, Penicillium expansum and Alternaria alternata were more sensitive than Rhizopus stolonifer and Botrytis cinerea with minimum inhibitory and fungicidal concentrations of 16 and 128 mg/ml, respectively.

One of the critical methods to maintain the stability and functional properties of plant essential oils as a useful source of bioactive compounds against environmental damage is their encapsulation in nanocarrier systems such as nanoliposomes. In this study, nanoliposome containing the citron peel essential oils were prepared without the use of toxic organic solvent and by employing health-giving compounds such as sesame oil in addition to lecithin for the first time in the formulation. The stability of the samples during 30 days of storage at temperatures of 4ºC and -18ºC was determined by investigating the retention amount of phenolic compounds, pH changes, antioxidant and antimicrobial performance. The nanoliposomal samples of essential oils of hydrodistillation and supercritical CO2 of citron peel prepared with different concentrations of lecithin oil had different quantities of pH and phenol retention percentage, and their amount reduced with increasing storage time at both test temperatures. DPPH inhibitory ability and antimicrobial activity of both citron peel essential oils were improved after encapsulation in nanoliposome. But their amount in both storage temperatures decreased with the advancing of time. The nanoliposome of the supercritical fluid essential oil of citron peel respectively with the formulation containing the highest and lowest amount of lecithin oil at the storage temperature of 4ºC showed the best result in this study. Therefore, the citron peel essential oil with encapsulation in the nanoliposome system prepared from lecithin-sesame oil, due to improvement of antioxidant and antimicrobial activity and its higher stability against storage temperature, can be used as an effective natural functional additive in the food industry.

Today, the extraction and use of biologically active compounds from agricultural and food wastes has received much attention. In the present research, the effect of enzyme treatment, ultrasound and the combined effect of enzyme treatment and ultrasound on the extraction of phenolic compounds of sour grape wastes was done so that 2 pectinase enzymes (Pectinex Ultracolor and Pectinex Yildamesh) at levels of 10, 20 and 30 mg/kg and ultrasound (times 10, 25 and 40 minutes) and (sound intensity 30, 60 and 90%) were used. The effect of enzyme treatment, ultrasound and the combined effect of enzyme treatment and ultrasound were investigated. According to the obtained results, the highest extraction efficiency belonged to Yaldemesh enzyme and with increasing sound intensity, the extraction efficiency increased significantly. On the other hand, increasing the extraction time led to an increase in extraction efficiency (p≤0.01). So that the highest extraction efficiency was observed in the sample extracted by Yaldemesh enzyme and under ultrasound at 90% sound intensity for 40 minutes (p≤0.01). According to the obtained results, the highest flavonoid, total phenol and antioxidant activity (DPPH, FRAP, ABTS) belonged to Yaldemesh enzyme, and with increasing sound intensity, antioxidant activity increased significantly (p≤0.01). On the other hand, increasing the extraction time led to a significant increase in antioxidant activity (p≤0.01). The highest antioxidant activity belonged to the sample extracted by Yaldemesh enzyme and subjected to ultrasound at a ultrasound intensity of 90% for 40 minutes and was introduced as the best treatment.

Eible films due to the environmental compability can be a good alternative to packaging made from oil materials. Today, consumers' concerns about the toxicity of artificial preservatives in the food industry have led to the search for natural antioxidants and antimicrobials sources. Ready-to-eat foods such as kebabs are known as the most susceptible foods for the growth of microbes and cause food poisoning.  The purpose of this study was to investigate the effect of an antimicrobial film of caseinates-cellulose nanocrystal containing supernatant and lactobacillus reuteri (PTCC 1655) on microbial and chemical properties of kebab. Therefore, a sodium caseinate-cellulose nanocrystal was prepared by adding 106 CFU/cm2 Lactobacillus reutri PTCC 1655 and germ-free supernatant, and mechanical properties evaluation tests, water vapor permeability, solubility and moisture were evaluated on the prepared films. The films were wrapped on kebab and each and every four days were monitored by microbial tests (total viable count, psychrophile bacteria and mold and yeast), chemical (pH, acidity, thiobarbituric acid and total volatile nitrogen, peroxide index). Among the treatments, the sodium caseinate -nanocellulose film containing Lactobacillus reuteri was the most effective treatment in increasing the shelf life of the kebab. As a result, it is suggested to use sodium caseinate-nanocellulose film containing Lactobacillus reuteri to increase the kebab shelflife.

Considering the sensitivity of fish meat to rapid spoilage, storage conditions and type of packaging are very effective factors in maintaining its quality and nutritional value after catching. In this research, rainbow trout fillets packed with aluminum and polystyrene as treatment and polyethylene bag as control were kept at 4°C for 12 days. Physical, chemical and microbial factors of fillets by recording changes in moisture, total protein, fat, total ash, free fatty acids, peroxide (PV), pH, Thiobarbituric acid (TBA), Total Volatile Basic Nitrogen (TVB-N) and also total microbial load It was measured. The results showed that with the passage of time, moisture and fat decreased significantly. Total protein, total ash, free fatty acids, PV, TBA, TVB-N and total microbial load increased significantly during storage. Also, pH had an increasing trend, but this increase was not significant. The highest amount of TVB-N on the twelfth day was related to the control package with a value of 24.52 mg/g. The lowest amount of total microbial load on the twelfth day was related to aluminum packaging with a log value of 6.86 cfu/g. According to the results, the priority of using packaging to increase shelf life and maintain the nutritional value of fillets was first with aluminum packaging and then with polystyrene. The polyethylene bag was declared unrecommended for consumption due to the lack of nutritional value and reduced product quality. Aluminum packaging worked significantly in maintaining the physicochemical and microbial properties of the product and according to the declared permissible limits of TVB-N and total microbial load, which are important indicators of spoilage, only fillets packed with aluminum containers could be consumed until the end of the storage period.