javad zavar reza

IQ motif-containing GTPase-activating protein3 (IQGAP3) contributes to the progression of bladder urothelial carcinoma (BLCA), but its mechanisms are not systematically specified. Due to the oncogenic potential of IQGAP3, the current in-silico study intended to elucidate IQGAP3's role in BLCA progression.

Many bioinformatics tools, including UALCAN, Kaplan–Meier plotter, TNMplot, cBioPortal, GeneMania, Enrichr, TIMER2, muTarget, and UCSC Xena, were applied in the current study.

The IQGAP3 level was more pronouncedly raised in BLCA tissues than in normal bladder tissues, and its increased expression was related to the advanced stage and higher grade. Enhanced IQGAP3 expression could result from its genetic alteration. Moreover, the mutation in P53 and RB1 genes was robustly associated with increased IQGAP3 expression. Besides, IQGAP3 correlative genes were dominantly involved in the cell cycle. On the other hand, IQGAP3 upregulation influenced immune checkpoint levels in the tumor microenvironment.

The in-silico findings suggested that IQGAP3 overexpression could be a crucial biomarker in BLCA.

This study aimed to assess the effect of low-level laser therapy (LLLT) on miniscrew stability and concentrations of interleukin-1β (IL-1β) and transforming growth factor-beta (TGF-β1) in peri-miniscrew crevicular fluid in the course of orthodontic treatment.

This randomized split-mouth double-blind clinical trial evaluated 18 patients requiring anterior retraction along with maximum anchorage. Miniscrews were placed between the maxillary second premolar and first molar. A diode laser was irradiated with a 980-nm wavelength and 100-mW output power in continuous-wave mode at four-time points: T0 (1 hour after miniscrew placement), T1 (1 week later), T2 (at 1 month) and T3 (at 3 months) in one quadrant of the maxilla (laser group). The other quadrant of the maxilla underwent the pseudo-application of the laser (control group). The primary stability of mini-screws was measured by Periotest M and reported as Periotest value (PTV). Also, at each time point, samples were collected from the peri-miniscrew crevicular fluid one hour after laser irradiation to assess the concentration of IL-1β and TGF-β1.

The mean PTV (inverse of the stability) was smaller in the laser group compared with the control group at all time points; this difference was significant at T2 and T3. The mean concentration of IL-1β in the laser group was lower than that in the control group at all time points, and this difference was significantly remarkable at T0 and T3. The mean concentration of TGF-β1 in the laser group was lower than that in the control group at T0, T1, and T3; however, the difference was not statistically significant.

The current results supported the efficacy of LLLT in increasing the miniscrew stability and decreasing the level of IL-1β pro-inflammatory cytokine

This new viral syndrome named as Coronavirus disease 2019 (COVID-19) by WHO. Some laboratory parameters change in the patients with covid-19. This study was conducted with the aim of evaluating laboratory parameters in the patients with COVID-19.

A cross-sectional-analytical study was conducted in the hospitals of Shahid Sadoughi of Yazd, Imam Jafar Sadegh of Meibod and Shahid Ziaei of Ardakan between April and May 2019. The study population was patients with Covid-19 with a positive PCR test, 275 people were included in the study using the available sampling method. The checklist of this study included demographic information, hospitalization information and test values (CBC, diff, ESR, AST, ALT, Cr, Urea, LDH, PT, PTT, INR). Data were analyzed by SPSS software version 17 and the significance level was considered less than 0.05.

A significant difference was observed between the values of WBC, Lymphocyte, Neutrophil, ESR, Urea, Creatinine, LDH, AST tests in the two groups that died and recovered. Among the patients who were hospitalized in the general departments, ICU or both, a significant difference was found between the patients who recovered and those who died (P=0.000). Significantly, more number of hospitalization days and older age were observed in deceased patients (P=0.000).

In the present study, we came to the conclusion that in addition to increasing the level of WBC, neutrophil, urea, creatinine, LDH and AST in deceased patients, a decrease in lymphocytes was also observed and these results can be used in future crises of Covid-19.

Hibiscus sabdariffa linnaeus (HSL) is a tropical plant with a high content of anthocyanin, traditionally found to have beneficial biological activities. This randomized, double-blind, placebo-controlled, and parallel trial was conducted to assess some renal and cardiovascular effects of supplementation with HSL in patients with diabetic nephropathy.

The study protocol was completed by 60 patients (38 females and 22 males) who were randomly assigned into the supplemented (SG) and Placebo groups (PG). The SG was treated with 425 mg of HSL twice daily and the PG received the placebo. Anthropometric and dietary information as well as fasting blood and urine samples were collected at the baseline and end of an 8-week intervention period.

Compared with the PG, supplementation with HSL significantly reduced systolic blood pressure (P = 0.01) and high-sensitivity C-reactive protein (P = 0.004). A significant increase was observed in total antioxidant capacity levels in the SG (P = 0.03). Moreover, we found a significant reduction in the levels of blood urea nitrogen (P < 0.001), blood creatinine (P = 0.002), urine creatinine (P < 0.001), and urine albumin (P < 0.001) in the SG compared with the PG. However, no significant change was observed in diastolic blood pressure, fasting blood glucose, and glomerular filtration rate following intervention between two groups.

HSL supplementation seems to be beneficial in improving the outcomes of patients with diabetic nephropathy; however, no considerable effect was observed on fasting glucose levels. Large-scale trials are needed to better understand its efficiency and safety for long-term use.

Chronic kidney disease (CKD) is one of the most threatening and important disorders worldwide in both industrial and developing nations. In addition, neutrophil gelatinase-associated lipocalin (NGAL), liver-type fatty acid-binding protein (L-FABP), and kidney injury molecule-1 (KIM-1) are three factors suggested as diagnostic and prognostic biomarkers in CKDs. Considering the lack of enough efficiency of the creatinine in the prognosis of the CKD, the present study aimed to assess the relationship between these three factors and CKD occurrence and determine if they could be considered valid biomarkers in this regard.

The present case-control study was designed enrolling 42 patients with confirmed CKD referring to the Imam Khomeini hospital of Kangan. The participants were 42 years old and gender-matched healthy counterparts. Blood samples were obtained, and then NGAL, KIM-1, and L-FABP were determined by the enzyme-linked immunosorbent assay using commercial kits (Bioassay Technology Laboratory). Finally, the serum creatinine was detected by applying Jaffe’s method.

Based on the results, significant differences were found in the serum levels of all four factors between CKD patients and the control group. More precisely, the serum levels of NGAL (P < 0.0001, specificity: 87.6%, sensitivity: 79.3%, and the area under the curve, AUC: 0.89), L-FABP (P < 0.0001, specificity: 83.3%, sensitivity: 78.3%, and AUC: 0.86), KIM-1 (P < 0.0001, specificity: 85.7%, sensitivity: 78.6%, and AUC: 0.88), and creatinine (P < 0.0001) were significantly higher in individuals with CKDs in comparison with controls. Eventually, the serum levels of NGAL, L-FABP, and KIM-1 were significantly correlated with each other in both patient and control groups (P < 0.0001).

In general, NGAL, L-FABP, KIM-1, and creatinine could be used as independent biomarkers for the diagnosis of CKD. Moreover, the measurement of NGAL, L-FABP, and KIM-1 altogether could be a valid assessment for the diagnosis of CKD.

Both canola and sesame oils consumption have been associated with favorable effects on cardio-metabolic biomarkers. However, to the best of our knowledge, no study has compared their effects on cardiovascular risk factors. The present study aimed to assess the effect of canola, sesame, and sesame-canola oils consumption on cardio-metabolic biomarkers in patients with type 2 diabetes mellitus (T2DM‎). This study was a randomized, triple-blind, three-way, crossover clinical trial. The study participants included 102 individuals with T2DM‎. Their spouses were also included in the study. The participants were entered into a 4-week run-in period. After that, their regular dietary oil was replaced with canola, sesame, or sesame-canola oils (a blend of sesame and canola oils) in three 9-week phases, which were separated by two 4-week washout periods (sunflower oil was consumed during the run-in and the washout periods). Dietary, physical activity, blood pressure, and anthropometric measurements were assessed at the beginning, middle (week 4-5), and end of each treatment phase. Blood samples were taken at the beginning and at the end of each phase. Serum, plasma, buffy coat, and whole blood samples were extracted and kept at -80 ºC for further analysis. Serum fasting blood sugar (FBS), triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol‎ (HDL-C)‎, and low-density lipoprotein cholesterol (LDL-C) were selected as the primary outcomes. 102 participants with T2DM were randomly assigned to one of the 6 rolling methods. Through them, 93 individuals (91.2%) completely participated in all phases. The present study will provide an exceptional opportunity to examine the effect of canola, sesame, and sesame-canola oil on cardio-metabolic markers in adults with and without T2DM‎. This trial will also provide a good medium for the investigation of gene-dietary oils interaction in the future.

Molybden, as a vital and essential micronutrient is directly involved in the metabolism of other elements including carbon, sulfur, and nitrogen. Molybdenum alone is not biologically active unless it binds to specific cofactors. Except for the bacterial nitrogenase, which contains molybdenum-Iron complex, molybdenum cofactor (Moco) is considered as the bioactive component placed in active site regions of molybdenum-containing enzymes. This review aimed to discuss the biological mechanisms involved in molybdenum metabolism highlighting Molybdenum cofactor deficiencies. Articles indexed in Pubmed, Google Scholar, and Scopus databases were used to extract the required information. Moco, as the cofactor of sulfite oxidase, xanthine dehydrogenase, aldehyde oxidase, and nitrite reductase plays a substantial role in maintaining normal body homeostasis and reactive oxygen species (ROS) production. Lack of Moco is found to be associated with many inborn genetic disorders, such as mental retardation, brain immaturity, nervous shocks, and neurodegenerative diseases. Moco insufficiency compromises normal human body metabolism since it is reported to regulate the metabolic pathways of other elements. Although in recent years, substitution- and gene-therapies have been introduced to restore the metabolic pathways of patients with MoCD type A and B, the definitive treatment for this type of inborn disease has still remained ill-defined. More investigations are needed to completely understand the underlying pathophysiology of molybdenum-related diseases.

Prostate cancer (Pca) is a heterogeneous disease, and current treatments are not based on molecular stratification. Poly(adenosine diphosphate [ADP]-ribose) polymerase (PARP) inhibitors have recently been found to be remarkably toxic to cells with defects in homologous recombination, particularly cells with BRCA-mutated backgrounds. Therefore, this preliminary study was designed to evaluate whether PTEN expression status could have an impact on the sensitivity of invasive Pca cells to the PARP inhibitor, AZD2461. MTT viability test, Annexin V‐FITC/propidium iodide double staining, and caspase3 activity assay were used to evaluate the apoptosis and relative expression of PTEN and VEGF in PC-3 and DU145 cell lines using real-time PCR. MTT results showed that the inhibitory effects of AZD2461 were higher in PC-3 than DU145 cells (with IC50 of 36.48 and 59.03 µM at 48 hours of treatment, respectively). Flow cytometric analysis also showed the same results. When exposed to 40 µM of AZD2461, PC-3 (38.8%) and DU145 (28%) cells underwent apoptosis (p < 0.05). Treatment of cells by AZD2461 also caused a significant increase in apoptosis through caspase3 activation in both cell lines. VEGF mRNA levels in PC-3 cells significantly decreased compared to adjusted untreated cells (p < 0.05) in all measured times while displaying different alteration patterns in DU145 cells (p < 0.05). AZD2461 suppresses the growth of prostate tumor cells since AZD2461 monotherapy could prove to be efficacious, especially against cells not expressing PTEN besides activating the possible apoptosis-independent cell death pathways.

Resistance to selective small-molecule inhibitors has been a substantial factor for limiting the efficacy of ovarian cancer. Recent studies have revealed that proteasome inhibitors induce acquired drug resistance. The possible mechanisms underlying the resistance to carfilzomib (CFZ), a recently developed inhibitor of proteasome, has not been well studied. This experimental study has aimed to determine if CFZ induces drug resistance in A2780 ovarian cancer cells through p53- and caspase-3 dependent pathways. The A2780CFZ cells were generated by continuous culturing of A2780S cells in the presence of CFZ for 4 months. The MTT cytotoxic assay was applied to compare the survival rates in A2780CFZ and A2780S cells. Also, the relative expression of p53 and caspase-3 genes were evaluated using quantitative real-time polymerase chain reaction (qRT-PCR). The nonparametric ANOVA and Friedman tests were used for data analysis. A significant difference was observed between the viability of resistant- and sensitive-A2780 cells exposed to various concentrations of CFZ, indicating that A2780S cells have become resistant to this drug under long-term culture. Compared with A2780CFZ cells, the mRNA levels of p53 gene in A2780S cells were significantly increased after 12 (P = 0.008), and 24 hours (P = 0.034) . Also, no significant differences were observed regarding caspase-3 mRNA levels between both cell lines (P > 0.05). The findings of this study suggest that regulation of p53 gene expression in A2780CFZ cells might be the possible primary mechanism for gaining resistance against CFZ, but this might be independent of caspase cascades activation. Further studies are required to find strategies for overcoming CFZ resistance.

Breast cancer (BC) is a complex disease, but current treatments are not efficient enough considering increased relapse and decreased survival rate among patients. Poly (ADP-ribose) polymerase inhibitors are recently developed anticancer agents which target cells with defects in homologous recombination (HR) pathway. This study wishes to assess whether the combination of AZD2461 as a newly developed PARP1 inhibitor and valproic acid (VPA), a histone deacetylase inhibitor could effectively reduce the growth of MCF-7 cells with no fundamental DNA repair defect. 

Both trypan blue dye exclusion assay and MTT viability test were used to evaluate cell death. γ-H2AX levels, as a marker of DNA repair, were measured using in cell ELISA method. The Student's t-test and non-parametric analysis of variance (ANOVA) were applied for our data analyses where p-value <0.05 was considered statistically significant.

As calculated by CompuSyn software, IC50 values for VPA and AZD2461 were 4.89 mM and 42.83 µM respectively following 48 hours treatment. Also, the trypan blue exclusion assay results showed a concentration- and time-dependent decrease when MCF-7 cells were treated with both agents (p<0.05). Combination analysis showed a mild antagonism (CI>1.1) while γ-H2AX levels found not to be significantly increased in MCF-7 cells co-treated with VPA+AZD2461 compared to each agent alone (p=0.29).

Our findings revealed that the combination of VPA and AZD2461 could decrease cell viability of MCF-7 cells, but it was not able to significantly increase unrepaired DNA damage sites. The mechanism responsible for drugs combination was not of synergism or addition. Determining the type of involved cell death mechanisms might be followed in further studies.

Treatments of advanced cervical cancer are limited to pelvic radiation and chemotherapy while outcomes are disappointing. Poly (ADP-ribose) polymerase inhibitors are highly toxic to cells with defects in DNA repair pathways. The purpose of the current study was to evaluate whether the combination of AZD2461 as a novel poly (ADP-ribose) polymerase 1 inhibitor and a histone deacetylase inhibitor, valproic acid, could be efficacious in Hela cells harboring no mutations in DNA repair pathways. Cell morphology assay and MTT viability test were performed to determine cytotoxic effects of AZD2461 and valproic acid, separately and in combination. The combination effects were measured using the Chou-Talalay's method. Although the analysis of cell morphology revealed that the combination of the two inhibitors could decrease the viable cells compared to each drug separately, MTT results showed that there was a mild antagonistic effect in the affected fractions of AZD2461/valproic acid-treated Hela cells at all effective doses (CI > 1.1). Our findings from this preliminary study conducted in Spring 2018 suggest that combining valproic acid with AZD2461 exerts mild antagonistic effects on Hela cells harboring no substantial defects in DNA repair pathways

According to the Public Beliefs and Chamomile combinations based on its relief effect, this trial is done to compare the effects of chamomile tea and black tea on depression and oxidative stress in depressed patients with diabetes. This is a randomized clinical trial that was done with participation of 74 depressed patients with diabetes. Participants were randomly divided to two groups of chamomile tea (CG) and black tea (BG) (n=37), that received 3 daily cups of chamomile tea and black tea bags respectively, at least half an hour after meals. Beck Depression Inventory was completed to assess depression at baseline, after 6 and 12 weeks. TAC and MDA tests were done at the beginning and end of the study. At the end of study, 64 patients completed the study. After the study, mean scores of beck decreased significantly between the two groups. Beck scores showed significant differences in both CG (P < 0.001) and BG (P = 0.04) after the intervention, but the mean changes in scores were Obtained significantly in CG more than BG. Mean TAC was significantly decreased in both CG and BG, the mean changes showed no significant differences between the two groups. This study showed that consumption of chamomile tea compared with black tea relatively decreased depression, but had no significant effects on oxidative stress.

Considering the effectiveness of green tea and Melissa officinalis in reducing weight and blood pressure in past studies, this study aimed to compare the effects of green tea and Melissa officinalis on blood pressure and weight in welders.
This is a single-blind crossover clinical trial. Twenty welders from a workshop entered the study. Green tea and Melissa officinalis infusions (4 g/d) were provided 2 times a day for 4 weeks in random order with a 3-week washout. Measurements were performed at the beginning and the end of each intervention. The environmental pollution of the workshop was measured by the professional health engineer at the beginning.
The diet and black tea intake were not different during the interventions (P > 0.05). Physical activity was not different throughout the study, although the intensity of physical activity was higher in the Melissa officinalis period (12.31 ± 12.89 versus 60.00 ± 33.95 min/ week; P = 0.06). Weight, body mass index and diastolic blood pressure decreased during the period of Melissa officinalis consumption and there was a slight increase during the green tea period (P > 0.05).
It is likely that one month of consumption of Melissa officinalis is as effective as green tea in changing weight and blood pressure, and therefore they can be a good alternative to each other

According to traditional beliefs, chamomile products have anti-depression effect. The aim of this study was to investigate the effects of chamomile tea on glycemic control, depression status and lipid profiles in type 2 diabetes (T2D) with depression.
This randomized clinical trial was carried out on 74 depressed patients with T2D. Participants were randomly divided into two 37-people groups, chamomile tea (CG) and black tea group (BG). The CG received 3 cups of chamomile tea daily and the BG received 3 cups of black tea daily half an hour after meals for 12 weeks. To examine the status of depression, Beck II test was utilized. Anthropometric measurements, 24-h dietary recalls, glycosylated hemoglobin A1c (HbA1c) and blood lipids profile were measured at the baseline and at the end of the intervention.
The HbA1c mean was significantly reduced in CG after the intervention, when compared with BG (7.15 ± 1.23% vs. 7.98 ± 1.76%, P = 0.02). In the same vein, the mean changes in CG and BG were -0.74 ± 1.29 and 0.04 ± 1.07 (P = 0.006), respectively. No significant changes were observed in mean of serum lipids within and between groups. The Beck score also showed a significant reduction in the CG after the intervention (P The results of this study demonstrated that drinking three cups of chamomile tea daily for 12 weeks by T2D suffering from depression lead to improve glycemic control and depression state. Therefore, drinking this kind of tea by these patients is recommended

Perchloroethylene is a halogenated solvent widely used in dry cleaning. International agency of research on cancer classified this chemical as a probable human carcinogen.
To evaluate the extent of primary DNA damage in dry cleaner workers who were exposed to perchloroethylene as compared to non-exposed subjects. The effect of exposure modifying factors such as use of personal protective equipment, perceived risk, and reported safe behaviors on observed DNA damage were also studied.
59 exposed and non-exposed workers were selected from Yazd, Iran. All the 33 exposed workers had work history at least 3 months in the dry cleaning shops. Peripheral blood sampling was performed. Microscope examination was performed under fluorescent microscope (400×). Open comet software was used for image analysis. All biological analysis was performed in one laboratory.
Primary DNA damage to leukocytes in dry cleaners was relatively high. The median tail length, %DNA in tail, and tail moment in exposed group were significantly higher than those in non-exposed group. There was no significant difference between smokers and nonsmokers in terms of tail length, tail moment, and %DNA in tail. There was no significant correlation between duration of employment in dry cleaning and observed DNA damage in terms of tail length, tail moment and %DNA in tail. Stratified analysis based on exposed and nonexposed category showed no significant relationship between age and observed DNA damage.
Occupationally exposure to perchloroethylene can cause early DNA damage in dry cleaners.

There is scarce data on the effects of omega-3 fatty acids and vitamin E co-supplementation on metabolic status in patients with fibrocystic breast disease (FBD). The current study was carried out to determine the effects of omega-3 fatty acids and vitamin E co-supplementation on metabolic status in patients with FBD.
A randomized clinical trial was conducted on 56 patients with FBD. Participants were randomly divided into two groups to receive either 1000 mg omega-3 fatty acids plus 400 mg vitamin E (n = 28) or placebo (n = 28) for 12 weeks. Fasting blood samples were taken at the beginning of the study and after 12 weeks of intervention to determine inflammatory factors, biomarkers of oxidative stress, and metabolic profiles.
After 12 weeks of intervention, changes in serum high-sensitivity C-reactive protein (-2171.4 ± 3189.1 vs. .9 ± 2774.8 ng/mL, P = 0.001) and plasma nitric oxide (.8 ± 4.0 vs. -0.1 ± 2.4 µmol/L, P = 0.04) in supplemented women were significantly different from those in the placebo group. In addition, compared to the placebo group, subjects who consumed omega-3 fatty acids plus vitamin E supplements had significantly decreased serum insulin concentrations (-3.2 ± 6.5 vs. -0.2 ± 1.7 µIU/mL, P = 0.01), the homeostasis model of assessment-estimated insulin resistance (-0.8 ± 1.7 vs. -0.02 ± 0.4, P = 0.03), serum triglycerides levels (-11.5 ± 47.3 vs. .6 ± 24.3 mg/dL, P = 0.03) and VLDL-cholesterol (-2.3 ± 9.5 vs. .1 ± 4.9 mg/dL, P = 0.03), as well as increased quantitative insulin sensitivity check index (.01 ± 0.01 vs. .001 ± 0.007, P = 0.001) and HDL-cholesterol (.4 ± 6.0 vs. -1.3 ± 4.3 mg/dL, P = 0.001).
Overall, omega-3 fatty acids and vitamin E co-supplementation for 12 weeks had beneficial effects on inflammatory markers and metabolic profiles in patients with FBD.

Iron is one of the nutrients that has recently received considerable attention because of its dual role in the incidence of breast cancer. The present study aimed at comparing hematological parameters, iron levels, and oxidative stress in women with and without breast cancer.

The participants in this case-control study were 55 women, of whom 26 were new cases of breast cancer (confirmed by biopsy) as the case, and 29 without cancer (confirmed by mammography) as the control group. All participants underwent blood testing for complete blood count (CBC (free iron, ferritin, total iron binding capacity) TIBC (2, 2-diphenyl-1-picrylhydrazyl (DPPH), and Malondialdehyde (MDA).

The mean±SD age of the participants was 44.25±9.82 years, and there was no significant difference between groups. Also, no statistically significant difference was found between the 2 groups in variables, except the mean corpuscular volume of red cells (MCV), mean corpuscular hemoglobin concentration (MCHC), and mean cell hemoglobin (MCH). The use of iron supplements was significantly higher in the control than in the case group (p= 0. 01), with an odds ratio of 0.19% (95% CI: 0.45-0.7). The mean serum DPPH was significantly higher in the control than in the case group (p= 0. 006), but comparison of serum MDA showed no significant difference between the 2 groups.

Iron deficiency anemia was greater in patients with breast cancer than in those without it. Moreover, iron supplementation appears to have a protective effect against breast cancer incidence. In addition, serum DPPH, as a total antioxidant index, was significantly higher in the control group.

Retinopathy is a microvascular complication of diabetes and leading cause of blindness throughout the world. The proteins as the most chemical substances in the cells are exposed to oxidative damages of free radicals. The aim of this study was to evaluate the values of protein oxidation biomarkers in diabetic patients with retinopathy.
This study was done on 30 type 2 diabetic patients with retinopathy as cases and 30 age and sex matched type 2 diabetic patients without retinopathy as controls. We measured HbA1c by Ion exchange chromatography and fasting blood sugar (FBS), cholesterol, triglyceride, urea, creatinine, HDL and LDL, protein plasma carbonyls (PCO), advanced oxidation protein products (AOPPS), SH groups and TAC by spectrophotometry in case and control groups and compared them. Urine micro albumin was measured in both groups and compared.
the mean of HbA1c, cholesterol, LDL-C, creatinine, PCO and AOPPS were significantly higher in cases (P-value The oxidative stress in diabetic patients with retinopathy caused elevation of protein oxidation and increased progress of diabetic disorders especially eye disorders.

recurrent implantation failure (RIF) is the most common cause of pregnancy failure after in vitro fertilization (IVF). Among the different causes of RIF, the role of maternal genetics factors is as one of most important factors. P53 codon 72 polymorphism is one of the notable polymorphisms in this field. According to evidences about the association between P53 polymorphism and increased risk of RIF in different population, if this relationship be justified in Iranian population, this polymorphism can be used to predict IVF success rate. This study was performed with aim to evaluate the association between this polymorphism and incidence of RIF.

This case-control study was performed on 80 infertile couples in Mashhad Montaserieh Infertility Treatment Center in 2010. The infertile couples were divided into three groups of control, RIF-1, and RIF-2. The control group included 40 women who were successfully pregnant after IVF. The patient group 1 were 20 women who no type of pregnancy was observed after two IVF, and patient group 2 were 20 women who failed to be pregnant after at least three IVF. P53 gene polymorphism genotype (Arg72pro allele) was determined using PCR- ARMS technique. Statistical analysis was performed by SPSS software (version 16) and Pearson Chi-Square test. P<0.05 was considered significant.

The frequency of Arg/Pro genotype in control group, patient group 1, patient group 2 and total patients was 92.5%, 75%, 80% and 77.5%. The frequency of Arg/Arg genotype in these groups was 7.5%, 25%, 20% and 22.5%, respectively. Pro/Pro genotype was observed in none of the groups. According to Pearson Chi-square test results, distribution of genotypes was not significantly different between patient group 1 and patient group 2 and also total patients in comparison with control group.

According to the results of present study in the studied population, there is no association between P53 codon 72 polymorphism and occurrence of RIF. So this polymorphism cannot be used to predict the risk of RIF.

Patients with metabolic syndrome are prone to cardiovascular disease and diabetes. Regarding the importance of nutritional factors in management of metabolic syndrome, this study was designed to evaluate the effects of dark chocolate consumption on serum lipid profile in patients with metabolic syndrome. In this randomized clinical trial, 114 patients with metabolic syndrome, aged 30 to 60 years, without heart, renal and hepatic diseases were recruited. The eligible patients were randomly allocated to receive either dark chocolate (76% purity), 20 or 40 grams daily for two months. Total cholesterol, high-density lipoprotein (HDL), triglycerides (TG) and fasting blood glucose (FBG) were measured with enzymatic methods before and after intervention. A low-density lipoprotein (LDL) level was calculated by the Friedewald formula. Data were analyzed using SPSS version 16.0 and ANOVA test P<0.05 was considered significant. Dietary intake was measured with NUT4 software at the beginning and the end of the study. There were no significant changes in dietary intakes of patients during the intervention in three groups. No significant differences were seen in mean concentration of lipid profile before and after intervention in groups (P>0.05). Consumption of 20-40g/day dark chocolate with 76% purity for 2 months doesnt change the lipid profile of patients with metabolic syndrome.

Type 2 diabetes mellitus (DM-2) is a chronic and progressive metabolic disorder known as a serious threatening condition in the individual and society. Type 2 diabetes mellitus is known as the major cause of cardiovascular diseases (CVD). Contradictory evidences about the effect of omega-3 fatty acids on CVD risk factors, particularly hypertension. The aim of this study was to determine the effect of omega-3fatty acids supplementation on blood pressure in DM-2 patients. This is a double-blind, placebocontrolled clinical trial on patients referred to Yazd Diabetes Research Center. Seventy DM-2 patients were randomly assigned to receive either 2 g/day omega-3 soft gels (OG) or 2 g/day placebo (PG) for 6 weeks. At the beginning and end of the study, blood pressure was measured and compaired Fifty nine percepts of patients in OG and 41% in PG had hypertension. Initially, there was no significant difference in the mean of age, body mass index (BMI), systolic blood pressure (SBP), diastolic blood pressure (DBP) and duration of diabetes between two groups. At the end of the study, the mean of SBP, DBP and differences between groups were not significant. Consumption of 2 g/day omega-3 supplement for6 weeks has no significant effect on systolic and diastolic blood pressure in DM-2 patients.

In this study we performed linkage analysis on a large X linked RSA pedigree to find a novel susceptibility locus for RSA. A linkage scan using 11 microsatellites was performed in 27 members of a large pedigree of hereditary X-linked RSA. Two point parametric Linkage was performed using Superlink v 1.6 program. Evidence of linkage was observed to markers at Xq23, DXS7133 and at Xq22.1 DXS101, with LOD score of 3.12 and 1.60, respectively. Identified locus in this study may carry a responsible gene in RSA. Narrowing down of this region may leads to identification of this gene Recurrent spontaneous abortion (RSA) is one of the most common health complications with a strong genetic component. Several genetic disorders were identified as etiological factors of hereditary X linked RSA. However, more genetic factors remain to be identified.

Macrophages derived foam cells are key factors in the maladaptive immune and inflammatory response.. The study of the cholesterol homeostasis and the molecular factor involved in these cells is very important in understanding the process of atherosclerosis and the mechanisms that prevent its occurrence.. This experimental study investigated the effects of c9, t11-Conjugated Linoleic Acid (c9, t11-CLA). Alpha Linolenic Acid (LA), and Eicosapentaenoic Acid (EPA) on the PPARα and ACAT1 mRNA expression by Real time PCR and cholesterol homeostasis in THP-1 macrophages derived foam cells.. Incubation of CLA, LA, EPA, and synthetic ligands did not prevent increasing the cellular total cholesterol (TC). Free cholesterol (FC) is increased by Sandoz58-035 (P = 0.024) and decreased by fatty acids and Wy14643 (Pirinixic acid) (P = 0.035). The pattern of distribution of %EC is similar to the EC pattern distribution. The ACAT1 mRNA expression was significantly increased by EPA (P = 0.009), but c9, t11- CLA, LA, Wy14643, and Sandoz58-035 had no significant effect on the mRNA level of ACAT1 expression compared to DMSO(Dimethyl sulfoxide).. In comparison to the control of Wy14643, Sandoz58-035, c9 and t11-CLA, EPA increased the PPARα mRNA levels (P = 0.024, P = 0.041, P = 0.043, and P = 0.004, respectively), even though, LA had no significant effect on the PPARα mRNA expression (P = 0.489).. Variations in the chemical structure of fatty acids can affect their physiological function..

Experimental studies have shown that walnut (Juglans regia) intake decreases the risk of coronary heart disease (CHD). Walnut decreases the levels of atherogenic lipids such as TG, LDL-C and VLDL-C. Mainly the effect is induced via 3- Poly Unsaturated Fatty Acids (3-PUFA). Walnuts are a rich source of these fatty acids, especially -Linolenic acid (C18:3; 9, 12, 15). We assigned 20 hypercholesterolemic male Rats (200-250g) to four groups, and fed with four diet concentration of oil extract Persian walnuts(J. regia)(Lavasanate) (w/w) as complementary diet: control group (0% oil extract) and cases 5%(1g oil extract/1g weight/1 day), 7.5%(1.5g oil extract/1g weight/1 day),10%(1g oil extract/1g weight/1 day) for eight weeks. Results revealed there is a positive effect on the decreasing of TG(14%), TC(7.8%), LDL-C(11%),VLDL-C(12%) serum concentrations, with increasing consumption of oil extract Persian walnuts (5%, 7.5% and 10%). In view of the positive effect of oil extract Persian walnuts (J. regia) consumption on decrease serum concentration of TG, TC, LDL-C and VLDL-C known as atherogenic lipids and lipoproteins, it may be suggested as a CHD protective dietary supplement.

Experimental studies have shown that walnut (Juglans regia) intake decreases the risk of coronary heart disease (CHD). Walnut decreases the levels of atherogenic lipids such as TG, LDL-C and VLDL-C. Mainly the effect is induced via w3- Poly Unsaturated Fatty Acids (w3-PUFA). Walnuts are a rich source of these fatty acids, especially a-Linolenic acid (C18:3; 9, 12, 15).
We assigned 20 hypercholesterolemic male Rats (200-250g) to four groups, and fed with four diet concentration of oil extract Persian walnuts(J. regia)(Lavasanate) (w/w ) as complementary diet: control group (0% oil extract) and cases 5%(1g oil extract/1g weight/1 day) ,7.5%(1.5g oil extract/1g weight/1 day),10%(1g oil extract/1g weight/1 day) for eight weeks.
Results revealed there is a positive effect on the decreasing of TG(14%) ,TC(7.8%) , LDL-C(11%),VLDL-C(12%) serum concentrations, with increasing consumption of oil extract Persian walnuts (5% ,7.5% and 10%).
In view of the positive effect of oil extract Persian walnuts (J. regia) consumption on decrease serum concentration of TG, TC, LDL-C and VLDL-C known as atherogenic lipids and lipoproteins, it may be suggested as a CHD protective dietary supplement.