The difference of med 13 gene expression of slow and fast twitch skeletal muscles due to endurance activity

Med 13 transcriptional factor effects on skeletal muscle response related to stimuli inducing muscular adaptation. The aim of this research was to study the effects of an endurance activity program on med 13 gene expression in slow and fast twitch skeletal muscles.
The subjects of this study were 14 wistar rats which were housed four weeks under controlled conditions before of start of protocol. Then they were randomly assigned to control and experimental groups. The experimental group performed an endurance activity program (fourteen weeks) on motorized treadmill. They were anesthetized and sacrificed 48 hours after the end of the last session of endurance activity program. The soleus and extensor digitorum longus muscles were removed. Using real time RT-PCR method, the rate of expression levels of med 13 gene determined. Finally, by using t-test, the collected data were evaluated.
The results of this research showed, physical activity significantly increase the expression of med 13 gene in soleus muscle but has no effect on expression of this gene in extensor digitorum longus muscles.
Despite the same endurance activity (duration and intensity), the rate of med 13 gene expression in fast and slow twitch muscles was difference which possibly return to difference of these muscles sensitivity to thyroid hormone.