THE ROLE OF ENDURANCE TRAINING INTENSITY ON THE PROTEIN EXPRESSION OF HSL IN THE SOLEUS MUSCLE OF STREPTOZOCIN-DIABETIC MALE WISTAR RATS

Increase in intramuscular fat contribute to the development of metabolic diseases, including type 2 diabetes. Endurance training with an effect on the lipolytic protein hormone-sensitive lipase (HSL) can also have a therapeutic aspect. The purpose of this study was to investigate the role of endurance training intensity on plasma insulin, fasting blood glucose, and HSL protein expression in Soleus muscle of type 2 diabetic male Wistar rats.

40 male Wistar rats aged 9-11 weeks and with an average weight of 230±24 (gr) were randomly divided into five groups(healthy control, diabetic control, diabetic+low intensity exercise, diabetic+moderate intensity training, diabetic+Extreme Exercise). Diabetes was induced by single-dose injection of 60 mg/kg STZ and 90 mg/kg nicotine amide intraperitoneally. The endurance training program was conducted for 8 weeks (5 sessions per week). The treadmill speed as an indicator of the intensity of training in the groups was 5-8, 14-17and 22-25 m/min. Plasma insulin and glucose levels were measured by ELISA and HSL protein expression by western blotting method. One-way ANOVA and Tukey's post hoc test were used to determine the significance.

Plasma insulin level in the high-intensity training group and fasting blood glucose in the moderate-intensity group decreased significantly compared to the diabetic control group(P<0.05). Also, HSL protein expression increased in the high-intensity training group compared to the diabetic control group (P<0.05).

Based on the results of this study, extreme endurance training seem to have a greater effect on plasma insulin levels and also increases the expression of HSL protein in Soleus muscle, but moderate training is better for controlling glucose and body weight in diabetic groups.