A Stereological Study on Colon Tissue Layers of Type 1 Diabetic Rats Following Thiamine and Lead Acetate Use

Diabetes is a well-known disease that has complications such as retinopathy, nephropathy and gastropathy. This study was designed to investigate the effect of thiamine and lead acetate on the colon of induced-alloxan diabetic rats, the effects of which become obvious in the treatment or reduction of tissue complications caused by diabetes.

In this study 63 rats weighing 200 g were divided into 9 groups:1) Group of diabetes+pb acetate 200 ppm; 2) Group of thiamin+pb acetate 200 ppm; 3) Group of thiamine+pb acetate 1000 ppm; 4) Group of diabetes+thiamine+Pb acetate 1000 ppm; 5) Diabetes group; 6) Group of diabetes+thiamine; 7) Group of diabetes+thiamine+acetate 200 ppm; 8) Group of diabetes+pb acetate 1000 ppm; 9) Control group. After a 20-day period, the samples were removed from abdominal cavity and the slides were prepared by using routine tissue method. Then, the slides were evaluated for the stereological and histomorphometric studies.

Ethical Considerations:

 This study was approved by the Code of Ethics GRN1M1903 at the Faculty of Veterinary Medicine, Shahrekord University, and all methods used in the present study, including facilitation, were conducted in full compliance with the ethical principles of animal restraint.

Mean thickness of mucosa-sub-mucosa showed significantly different in groups 6 and 7 compared to other treatment groups. There was a significant difference in the thickness of the muscle layer between the control and all treatment groups except for groups 2, 6 and 7. There was no significant difference in the mean thickness of advantia layer in groups 1, 7 and 8 with the control group. The results also showed a statistically significant difference in the area of different layers of colon tissue between group 1 and control group.

Based on the results of this study, it seems that thiamine has enhancing effects on muscle layer thickness and adventitia layer thickness. On the other hand, the area of the mucosal layer was not affected by the improving effects of thiamine.