Evaluation of Cytotoxic and Antibacterial Effects of Zanjani Snake Venom in vitro

Due to the resistance of some pathogenic micro- drawn researchers' attention to the use of antibacterial drugs developed from natural sources, especially venomous animals such as snakes. In this study, for the first time, the antibacterial effects and cytotoxicity of Zanjani snake venom (Vipera organisms to common antibiotics, treatment of microbial infections has become significantly more difficult. This has albicornuta) were investigated in vitro.

In this in vitro experimental study, the antibacterial effect of different concentrations of Zanjani snake venom on Staphylococcus aureus, Escherichia coli and Bacillus subtilis was investigated using MTT assay, MIC assay, disk diffusion and well methods. In order to evaluate the cytotoxicity of crude toxin, HepG2 cells were treated with different concentrations of 50, 100, 200 and 400 μg / ml of crude toxin for 24 hours. Survival percentage was determined using MTT reduction and Neutral red uptake methods and cell death was determined by Comet Assay method.

According to the results, crude toxin has no antibacterial effect on Escherichia coli but has a significant antibacterial effect on Bacillus subtilis and Staphylococcus aureus. The results also showed that Zanjani snake venom at doses of 50 to 400 μg / ml induces cell death by inducing apoptosis and necrosis.

In this study, we showed for the first time that Zanjani snake venom has antibacterial and cytotoxic effects and can be used for further studies.