Evaluating the effect of 0.05% fluoride and chlorhexidine 0.2% mouthwashes in comparison to nanosil D1+F mouthwash on Streptococcus mutans, in vitro

Oral diseases, especially dental caries, are still prevalent in the most developing countries which cost a lot for patients and the health care system. One of the most important ways to reduce costs is to prevent caries. Therefore, the aim of this study was to evaluate the effect of chlorhexidine and fluoride mouthwashes in comparison with Nanosil D1+F combined mouthwash on Streptococcus mutans in vitro.

In this experimental study, well diffusion method was used to evaluate the diameter of the growth inhibition zone of the mouthwashes (chlorhexidine 0.2%, fluoride 0.05%, nanosil D1+F). Then, MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration) of the mouthwashes were evaluated by the standard microdilution method.

Based on the results of the well diffusion method, the diameter of the growth inhibition zone for fluoride, chlorhexidine, and nanosil mouthwashes was 9, 18 and 32 mm, respectively. MIC values were 125 mg/ml for fluoride mouthwash, 3.9 mg/ml for chlorhexidine mouthwash and 1.95 mg/ml for nanosil D1+F mouthwash. The MIC and MBC values were the same for each mouthwash.

According to the results of the present study, Nanosil D1+F mouthwash had stronger antimicrobial effects on Streptococcus mutans than chlorhexidine 0.2% and fluoride 0.05%.