Evaluation of the effect of warfarin consumption with continuous and interval training on the expression of cardiac osteocalcin, vitamin K2 (MK-4) and serum calcium in a model of rats with myocardial infarction

Exercise can minimize the risk of myocardial infarction (MI) by regulating cellular signaling in the heart and arteries. The aim of this study is to evaluate the effect of warfarin consumption with continuous and interval training on the expression of cardiac osteocalcin gene, vitamin MK-4 and serum calcium in myocardial infarction mice.

In this experimental study, 42 male Sprago-Dovali rats (180-120g) were randomly divided into 7 groups: healthy control, myocardial infarction or ischemia (ISC), ISC+interval training, ISC+continuous exercise, ISC+warfarin, ISC+interval training+warfarin and ISC+continuous exercise+warfarin. Induction was induced by subcutaneous isoproterenol. The duration of training was eight weeks, five sessions per week and the dose of warfarin was 0.5 mg/kg per day. Gene values, changes in vitamin K2 and calcium were analyzed by Real time-PCR, ELISA, photometric and data analysis using independent t-test, one-way variance and two-factor variance (P<0.05).

Disease induction increased the expression of osteocalcin (p=0.003), vitamin K2 (p=0.027) and calcium (p=0.001) genes compared to healthy controls. In the study of osteocalcin gene expression, ischemia+warfarin group with ischemia+EIT (p=0.014) and ischemia+ECT (p=0.007) and in the study of calcium levels of ischemia group with ischemia+EIT (p=0.014), Ischemia+ECT (p=0.001) and ischemia+warfarin+ECT (P=0.013) were significantly different. Also, the interaction between exercise and drug was not confirmed on any of the variables.

It seems that interval and continuous exercise be effective in reducing vascular degradation and calcification after MI by reducing the cardiac osteocalcin and serum calcium and minimizing the side effects of some drugs such as warfarin.