Evaluating the Level of Expression of CXCR4 Receiver after Silencing by siRNA in MDA-MB-468 Cancer Cells

Many studies have shown that siRNAs can silent the genes involved in growth and invasion of cancer cells. However, the exact effect of siRNAs is not entirely clear on the expression of CXCR4 gene in breast cancer cells. Therefore, the present study evaluated the expression of CXCR4 gene after silencing the siRNA in metastatic breast cancer cells. In this experimental-laboratory study, MDA-MB-468 breast cancer cells were transfected by the specific siRNA of the snail1 gene. The expression levels of CXCR4 gene were evaluated by qRT-PCR. Cell proliferation was assessed by trypan blue test. Data was analyzed using t-test. Snail1 knockdown by siRNA significantly reduced the expression level of CXCR4 in MDA-MB-468 breast cancer cells (P<0.05). The results of this study showed that the transfection of breast cancer cells by the specific siRNA of snail1 gene can reduce the expression level of CXCR4 and thus reduce the proliferation and invasion of breast cancer cells.