Genotypic study of resistant Staphylococcus aureus strainsTo methicillin isolated from patients in Shahrekord

isolated from patients' infections and to determine the pattern of antibiotic susceptibility in patients referred to Shahrekord hospitals.

Antibiotic susceptibility model was performed by disk diffusion method based on CLSI model. Isolated isolates were analyzed by PCR for the presence of mecA target gene and confirmation of SCCmec typing.

45 patients (27.10%) out of 166 patients had positive culture of Staphylococcus aureus, of which 11 (24.44%) were male patients and 34 (75.55%) were patients. Oxacillin resistant and 36 isolates (80%) were resistant to cefoxitin and 45 isolates (100%) were phenotypically MRSA. These isolates were confirmed by molecular method (presence of mecA gene carrying molecule by PCR).100% of patients with Staphylococcus aureus infection were methicillin resistant and 95.5% were MDR. Of 100 methicillin-resistant isolates (isolates containing mecA gene), type V was detected in 53.33% of isolates. Also, TypeIII and Typr IVa were detected in 26.66% and 17.77% of isolates, respectively.

More than half of the staphylococcal infections in this study were caused by Staphylococcus aureus and more than 48% of the isolates were resistant to methicillin. The highest resistance to penicillin was observed. The high prevalence of Staphylococcus aureus is a serious danger to society.