The effect of three methods of HIT, HIIT and MIT training on leptin and lipoprotein lipase gene expression in subcutaneous fat tissue of male rats.

Leptin and lipoprotein lipase are important factors in the process of fat metabolism and energy homeostasis, and the effect of different methods of exercise training has been less studied. The aim of this study was the effect of three types of HIT, HIIT and MIT exercises on leptin and lipoprotein lipase gene expression in the subcutaneous fat tissue of male rats.

32 male rats with an average weight of 237 ± 25 grams were prepared. Mice were randomly divided into four equal groups of control, moderate-intensity aerobic training, high-intensity aerobic training, and high-intensity intermittent aerobic training. The training groups participated in training programs for 8 weeks. The expression of leptin and lipoprotein lipase genes was determined using RT-PCR laboratory method. To investigate the difference between groups of variables, one-way analysis of variance and Tukey's post hoc test and SPSS-24 software were used..

The results showed that there was a significant difference in the expression of leptin and lipoprotein lipase between the groups (control, HIT, HIIT and MIT)(p=0.001).Tukey's test results showed that although performingHIT, HIIT and MIT exercises decreased leptin gene expression (p=0.001) and increased lipoprotein lipase compared to the control group(p=0.001).However, no significant difference was observed between training groups(HIT, HIIT and MIT)in leptin and lipoprotein lipase changes.

The results of the present study showed that HIT, HIIT and MIT all cause changAes in leptin and lipoprotein lipase gene expression, but it seems that the changes in leptin and lipoprotein lipase in HIT exercises were more than in MIT and HIIT.