فصلنامه آسیب شناسی درمانگاهی دامپزشکی
سال سیزدهم شماره 1 (پیاپی 49، بهار 1398)

This study is for the effect of Tilia extract on gene expression of caspase 3 and caspase 9 in canine mammary gland cancer cell line. During this survey Tilia is extracted and has been farming and passage the mammary gland cancer cell line CF41.Mg. After that treating the cell lines in the 24 ,48 , 72 hours with dosage 5 ,10 , 25 ,50 , 100 mg/ml has been done from the essence of the Tilia. Regarding to the examining of the alive cells have been used through the MTT assay approach. For understanding of the involving mechanism to inspire the death of the cells in the referring period of time and RNA was extracted and cDNA was spread. The rate of explaining the genes cas3, cas9 through the use of the special primers in them whit RT-PCR and also qRT-PCR has been studied. According to the result activeness of the anti tumor of this extract after 48 hours whit dose of 25 mg/ml has been effective. More over , the conclusion of the examination of RT-PCR symptom of the description about the cas3,cas9 genes in treating sample among 24 ,48,72 hours . The consequences of this survey has verified and tells the effect of the control flavenoids on the extract of Tilia in reproduction of the cell lines CF41.Mg .

Diabetes cause malfunction in genital system such as disorder in spermatogenesis , decrease of sperm , serum testosterone , and seminal fluid amount . In this study , 30 male wistar rats divided into three groups of 10. First group was control , the second was diabetic and the third one was treatment .Treatment group became diabetic by using streptososin , and recieved oleoreupien for 28 dasys with dosage of 500mg per day by using gavage. In the end of testing period ,animals disected and samples of prostate and vesicular glands were obtained and propered for histological studies . Results and Morphological studies show increase in secration , diameter of secretory units ,thickness of muscular layer and dacrease in interstitial connective tissue in prostate and vesicular glands in treatment group in contrast with diabetic group. Changes of epithelium from columnar and cuboidal to spindle , were less than diabetic group. Histomorhology results show meaningful increase in epithelium's height and prostate tubules diameter also increase in muscular layer thickness and vesicular glands epithelium's height in treatment group in contrast by diabetic group.(P<0.05) Also it's revealed that in treatment group in contrast by diabetic group ,meaningful increase in ratio of weight of glands to body weight is clear.(P<0.05) In this study revealed that Oleoreupien's extract in diabetic male rats, decrease the rate of tissue disorders in prostate and vesicular glands. Also improve their functioin. Keywords : Oleoreupien , diabetes , prostate , vesicular glands , rat

Due to the accumulation of lead metal in the fish's edible tissues, the safety and health of human food are also affected. This study was conducted to compare Ascorbic acid and Thiamine in prevention of tissue damages due to lead in some tissues of common carp (Cyprinus carpio). Fish were divided randomly into 4 groups of 30 fish each. Group 1 considered as control group. Group 2 received lead acetate, group 3 and 4 respectively received thiamine and ascorbic acid in addition to lead. After necropsy, tissue specimens including brain, kidney and liver were prepared, and processed slides were stained with Hematoxylin-Eosin. Lesions in brain including hyperemia, edema and ischemic cell changes. Score of brain lesions were significantly lower than groups 2 and 3. In liver, hyperemia, hyperplasia of melano-macrophage centers, hepatocellular degeneration and intranuclear acid-fast inclusion bodies were observed. Severity of Hyperemia and degeneration in group 3 had a significant decreased in comparision to group 4. In the statistical comparison none of pathological indices in kidney including hyperemia, hemorrhage, intranuclear acid-fast inclusion bodies, necrotic changes, swelling of epithelial cells, hyperplasia of melano-macrophage centers, revealed significant difference between group 3 and 4 in kidney, but compared to group 2, some lesions in these groups, showed a significant difference. Based on the results, it can be concluded that ascorbic acid and thiamin might have some protective and therapeutic effects on lead poisoning in fish species

The causative agent of theileriosis in cattle, is obligatory intracellular protozoa that is called T.annulata and result in severe anemia, severe pulmonary edema and in non-native animals rapid death.The present study was aimed to detect the current state of Theileria, in buffalo and their environmental factors associations in four great cities of West Azarbaijan province, Iran, using microscopic tests (Blood smear evaluation) and molecular assays. Randomly, 291 blood samples were collected from buffaloes in the region, during March to July 2014. Direct and Semi-nested PCR techniques were used to detect DNA of Theileria spp. using specific pair primer 18SrRNA. In microscopic assay method, presence of this parasite in four (1.37 %) buffalos was approved. The molecular assays revealed that in total buffalo samples 4 out of 291 animals (1.37%) were infected to T.annulata. Also the present study showed that two female buffaloes (0.68%) were simultaneously infected to T.annulata and T.orientalis. Apparent and real prevalences of infection of buffaloes to T.annulata in molecular method were 1.37 and 1 percent, respectively. The chance of infection to both T.annulata and T.orientalis were the same. Molecular prevalence of T.annulata in Urmia and Salmas were 2.2 and 2.3 respectively (P<0.05). Molecular prevalence of T. orientalis in Urmia was 2.2%. This study showed the first record of the buffalo infection with T.orientalis parasite in Iran.

Survey of ceftiofur hydrochloride on dairy cow uterine health that had been affected by placenta retention and dystocia had been done and also fertility rate of both group counted. A total of 300 dairy cow has been divided equally in two groups. All cows affected by dystocia and placenta retention randomly have assigned in two groups.in the treatment group ceftiofur hydrochloride injected 2.2mg/kg SC during 5 days and in the control group cows that had placenta retention oxytetracyclin injected intra uterus 24h after parturition and also oxytocin 50 IU before OTC has injected 2times every 8 h after parturition and cows that had dystocia in the control group did not receive anything. ceftiofur hydrochloride in the treatment group decreased rate of infections in comparison of control group significantly. (p<0.01)Also open days in the treatment group was lower than control group significantly. (p<0.05)First service rate was lower in the control group significantly. (p<0.01) The results had shown that preventing of uterine infection and metritis is inevitable part of dairy cow transitional period. ceftiofur hydrochloride injection as a preventing drug can be utilize in the farms that have dystocia and placenta retention abnormally before every control program for reducing these disorders.

Abomasal hypomotility plays an important role in pathogenesis of some abomasal disorders such as abomasal bloat that there are same serious side effects associated with using synthetic drugs for its treatment, such as diarrhea and antibiotic resistance and for decreasing these side effects, administration of herbal medicine can be appropriate approach. Evaluating effect of Folus fruit aqueous extract on lamb,s abomasal emptying is goal of this study. This study was conducted on twelve five-day-olds Sangsari-female-lamb (average weight 4 kg). lambs received five oral treatments including salin (30ml), Eeythromycin (400 mg), Folus (700 mg/kg), Folus (750 mg/kg) and Folus (800 mg/kg), respectively Acetaminophen absorption test was used to evaluating the rate of abomasal emptying. After drawing relational model between plasma Acetaminophen concentration and time with regression method showed that treating with erythromycin and three different doses of aqueous extract of Folus fruit (700, 750, 800 mg/kg) increased the rate of abomasal emptying in comparison to the negative control, significantly (P<0.05). No clinical side effects were observed following the administration of erythromycin and Folus in lambs. This study showed that aqueous extract of Folus fruit has a stimulatory effect on lamb's abomasal emptying but more studies are needed on the effect of this plant components on abomasal emptying.

Lipopolysaccharide (LPS) is an important factor that induces adverse developmental outcome. They include intrauterine growth retardation (IUGR), intrauterine fetal death (IUFD), embryonic resorption, and preterm delivery in rodents, and were related LPS-induced oxidative stress. In this study, 48 pregnant female rats were divided into 4 groups and injected intraperitoneally from lipopolysaccharide to 15 and 17 days pregnant, respectively, to the first and second groups of 75 micrograms per kg body weight. The second and third groups received 100 mg / kg vitamin A intramuscularly a week before injection of lipopolysaccharide. The fourth group was the control group and placebo was injected to simulate injection stress. On the 18th day, all the rats were euthanized. The number of live fetuses, dead fetuses, and resorption sites was counted. Live fetuses in each litter were weighted. Crown-rump and tail lengths examined and skeletal development was evaluated. In addition, maternal liver, placenta, and fetal liver samples were excised for measurement of MDA and GSH contents. Results show administration of LPS significantly increase fetal mortality, decrease fetal weight and crown-rump and tail lengths of live fetuses and retarded skeletal ossification in caudal vertebrae, anterior and posterior phalanges and supraoccipital bone. Our study showed that co-treatment of vitamin A and LPS could decrease defects of LPS and improve injuries.

Identification and epidemiological survey of fungal infections at the hatcheries has great importance for prophylaxis of high mortalities and subsequence economic lost. In this survey, 5 rainbow trout hatcheries in the West Azarbaijan were investigated. Sample collection had done according to sampling tables suggested by Veterinary Organization of Iran. Water quality of hatcheries investigated as well. SDA) Sabouraud dextrose agar (medium used for fungal culture and identification of fungal type had done based on microscopic examination of fungi reproductive organs. In our survey 5 hatcheries had fungal infection of 4 families as follow: Saprolegnia sp., penicillium sp., Aspergillus sp. and Achyla sp. .Genus Saprolegnia with (7.8±1.48) infection rate had highest prevalence and Achyla (0.8±0.83) had lowest prevalence. Water quality of hatcheries was at acceptable conditions and fungal affection is probably irrelevant to the water quality. According to result of our study, hatcheries of West Azerbaijan province are at worse sanitary and fungal infection conditions.