دکتر فرهنگ حداد

In the present study, the antioxidant effect of vitamin C on decreasing the induced chromosomal damages by low-frequency electromagnetic field on bone marrow erythrocytes of male Balb/C mouse has been investigated. In this experimental study, 48 adult male mouse (Balb/C) were randomly divided into four equal groups: control, test 1, test 2 and test 3. Control group were kept in normal conditions and the mice in test1 and test3 groups were intraperitoneally injected with 50 mg / kg of vitamin C for 8 consecutive days. Then the mice of test 3 and test 2 groups after the fifth day were exposed to electromagnetic waves with frequency of 50 Hz and intensity of 50 Gauss for 4 days and 4 hours per day. The mice in all different groups were sacrificed and micronucleus test were performed on the bone marrow polychromatic erythrocytes. The data were analyzed using spss software and ANOVA test at level p < 0.05. The frequency of micronucleus in bone marrow polychromatic erythrocytes of test3 group (3.107± 0.276) in comparison with test 2 group (9.29 ± 0.881) showed a significant reduction (p < 0.001). Vitamin C caused significant decrease in induced chromosomal damages by low-frequency electromagnetic waves in bone marrow polychromatic erythrocytes of adult male Balb/C mouse.

Green tea due to its antioxidant properties can prevent some types of chromosomal damage. In this study, the effects of green tea on repressing chromosomal aberrations induced by cell phone waves in bone marrow polychromatic erythrocytes of male Balb/C mice were investigated. In this experimental study, 40 adult male Balb/C mice were randomly divided into five groups: control (natural conditions), sham exposed (no exposure to cell phone waves in vitro), experimental group 1 (exposure to cell phone waves), and experimental group 2 (intraperitoneal injection of 100 mg/kg dose of green tea extract for 5 days and exposure to cell phone waves), and experimental group 3 (intraperitoneal injection of 200 mg/kg dose of green tea extract for 5 days and exposure to cell phone waves). Micronucleus test was run in all groups. Data were analyzed by ANOVA and Tukey tests using SPSS software (p<0.05). The mean number of micronucleus in polychromatic erythrocytes of the sham group (3.47± 0.253) compared with control group (3.43 ± 0.605) did not show a significant difference (p> 0.05), whereas the mean micronucleus frequency in the experimental group 1 (5.64±0.308) increased significantly. However, the mean micronucleus frequency in experimental group 2 (0.92 ± 0.129) and experimental group 3 (0.55 ± 0.046) compared with the control and sham exposed groups decreased significantly (p = 0.001). Cell phones microwaves (940 MHz) induce chromosomal aberrations in bone marrow polychromatic erythrocytes of male Balb/C mice, but green tea has inhibitory effects and reduces chromosomal damage.

In recent years, the widespread use of microwave producing instruments specially cell phones; result in growing concern regarding the possible effects associated with these waves on human health especially pregnant woman and neonates. In present study, we investigated the genotoxic effects of cell phone radiation on the mice (Balb/C) and their offspring. In this experimental research, pregnant mice were irradiated with cell phone for 4 days of gestational age (days 14th-18th, 6h per day, from 9am until 3pm) and after litter, 2nd-day offspring studied for morphology, weight and CR length. By following, for assessment of possible genetic damages in erythrocytes after bleeding from heart, smears of spleen tissue prepeard for histological studies. Mice peripheral blood and bone marrow smears prepared and stained with May-Granowald and Gimsa. The finding in experimental group indicated that cell phone radiation decreased offsprings’ weight and CR length (p<0.001). Megakaryocytes in marrow (p=0.02) and red blood cells in spleen (p=0.03) were increased but were not shown any changes in lymphocytes (p>0.05). An increase in micronucleus frequency in peripheral blood erythrocytes were seen in experimental newborn (p=0.006) and adult mice (p<0.001) but we did not found any change in the frequency of micronuclei in adult mice bone marrow polychromatic erythrocytes (p>0.05). Above findings indicated that cell phone radiation (940 MHZ) are able to increase the frequency of micronucleus in peripheral blood erythrocytes of adult mice and their of fsprings and induce a genotoxic response.

This study examined the effects of vitamin A Chromosomal damage induced by the inhibition of cell phone radiation on the bone marrow erythrocytes on mice. In this experimental study 48 male rats BALB/c were dividedrandomly into four groups: control, laboratory sample1, 2and the empirical distribution, the control mice were kept in natural condition, samples were first for four consecutive days and every day for three hours (12-9) in vitro and cell phone signals within the passive mode (OFF), were studied The mice in laboratory sample 2 were exposed to mobile phones in vitro within three hours (12-9) for four days and the samples of experimental group were injected with15,000 Iu/kg vitamin A intraperitoneally for five consecutive days and on the second day they were exposed to mobile phone inactive mode (ON)for four consecutive days and every day for three hours (12-9). All different groups of mice were described and micronucleus test of polychromatic erythrocytes in bone marrow of mice was performed. Quantitative data obtained with software Spss and were analyzed by ANOVA test (p <0.05). The frequency of micronucleus in polychromatic erythrocytes in bone marrow of experimental mice (25/23) compared with controls (68/34), group 1 (88/35) and control 2 (55/45) showed a significant reduction said. The group 2 compared with control groups has shown significant increase in the number of micronucleus. Vitamin A reduces the in duction of chromosome damageinduced by waves in the mobile mouse bone marrow polychromatic cerythrocytes of adult male BALB/c.

Electricity is one of the advanced and technology keys. In recent decades using of generating devices of electromagnetic fields has led to placed humans exposed to electromagnetic fields. Vitamin E is one of the soluble antioxidant in fat that has an important role in lipoproteins، the biological membrane، nervous system، reproductive system and antioxidant chain the is placed against oxidative damage. In this research the antioxidant effects of vitamin E on the repressing of induced chromosomal damages by extremly low-frequency electromagnetic field on bone marrow erythrocytes of adult male Balb/C mouse has been studied. In this experimental study، 31 adult male Balb/C mice were divided into 3 groups control، shamexposed and experimental groups. control group were kept in natural conditions، Mice in sham- exposed group were exposed to low electromagnetic field intensity 50Hz for 4 days، each day 4 hours and the mice in experimental group were injected intraperitoneally، vitamin E with dose of 200 Mg / Kg for 8 consecutive days، and the fifth day، these mice were exposed to electromagnetic fields intensity 50 Hz for 4 days، each day 4 hours. after completion of treatment، all mice were dissected and micronucleus test was evaluated in poly chromatic erythrocytes of bone marrow. Quantitative data obtained using of Spss software and ANOAA test at the level of pResult