جستجوی مقالات مرتبط با کلیدواژه « سلول های بتا » در نشریات گروه « پزشکی »

The role of Wnts as mediators of pancreatic development has been confirmed, and exercise training may affect this process. Therefore, the present study aimed to investigate the effect of eight weeks of resistance training and moderate-intensity training on plasma Wnt3a, Wnt4, and pancreatic beta-cell count in streptozotocin-induced diabetic rats.

Twenty-four rats were divided into four groups: healthy control, diabetic control, diabetic + moderate-intensity continuous training, and diabetic + moderate-intensity resistance training. Diabetes was induced by intravenous injection of 110 mg nicotinamide and 40 mg streptozotocin per kg of body weight. The exercise training intervention was performed for eight weeks. Plasma Wnt3a and Wnt4 were measured by the ELISA method, and the number of beta cells was assessed by hematoxylin-eosin.

Data analysis showed that the number of beta cells in the exercise group increased significantly compared to the diabetic control group (P<0.05). Also, a significant change was observed in the plasma levels of Wnt3a and Wnt4 in the exercise group compared to the healthy control group (P<0.05).

It seems that eight weeks of moderate-intensity continuous training and eight weeks of moderate-intensity resistance training probably caused the proliferation and protection of beta cells in response to an increase in Wnt3a and a decrease in Wnt4 in the diabetic rats.

Mesanchymal stem cells (MSCs) derived from a variety of human adult tissues and potentiate to self-replicate and differentiate into different cell types. Retinoic acid (RA) is important in embryonic development of pancreas. The effect of RA on transdifferentiation of rat bone marrow mesanchymal stem cells (BMMSCs) into the beta cells was studied.

Rat BMMSCs were prepared by flashing method and confirmed by evaluating of BMMSCs surface markers. BMMSCs were treated in four groups by: 1) rat pancreatic extract (RPE) (250µg/ml) 2) RPE (250µg/ml) + RA (10µM), 3) no treated group as control and 4) ethanol 10% as vehicle and RA(10µM) as control. Insulin secretion was evaluated by ELISA assay. Insulin expression (RT-PCR) were determined.

RT-PCR results showed that insulin expression in RPE and RPE + RA groups. Insulin releasing in group RPE + RA was significantly more than group 1, (p-value < 0.05), Wilcoxon test..

Our study showed that RA can promote differentiation of the BMMSCs into the insulin producing cells invitro.

Betatrophin known as new therapeutic target of pancreases tissue. The aim of this study was to investigate the effect of endurance training program on betatrophin serum concentrations and pathological changes of pancreas tissue in diabetic rats.
Material and Male wistar rats (N=24) divided in 3 group include healthy control, diabetic control and endurance training. After induction of diabetes, training group performed 4 weeks endurance training and betatrophin concentration was assessed by ELIZA and The pancreatic tissue was evaluated via histological method.
Results showed that betatrophin serum concentration significantly decreased in diabetic control group (P≤0.05) but there was no significant increases observed in training group (P≥0.05). Compared with diabetic control groups, endurance training didn’t make any significant changes in insulin resistance and beta cell function (P≥0.05). In endurance training group, the mean of number and size of pancreatic islets and number of β-cell was higher than diabetic control group.
Although endurance training didn’t able to significantly increase the betatrophin levels but it had a positive effect on the pancreatic tissue in diabetic rats

Since aloe vera plant has many medical benefits, the present study aimed to investigate the protective effects of Aloe vera gel on the pancreatic islets and beta cells. This experimental study consisted of 50 mature male rats aged 2-3 months and weighed 200-250 g, who were randomly divided into five groups (n=10). Group I (control) did not receive any treatments, and group II were diabetized via Streptozotocin (IP) in 65 mg/kg, whose blood sample was taken after one week. Rats with blood glucose more than 250 mg/dL were considered as diabetic. Group III diabetic rats received the Aloe vera gel daily with dosage of 400 mg/kg, and group IV diabetic rats received insulin in 10 units/rat. Group V involved healthy rats which received only Aloe vera gel. After the last Aloe vera gel administration, blood glucose and body weight of all groups were measured on 15th and 30th days. Animals were euthanized with ether. Then tissues samples were collected from pancreas gland and fixed in 10% neutral buffered formalin solution. The 5-6 µ sections were made by paraffin embedding method and stained using haematoxylin-eosin (H&E) and Aldehyde fuchsin stains. Ultimately, the histomorphometrical parameters were evaluated. The mean number and size of pancreatic islets and beta cells of Langerhans islets decreased significantly in the diabetic group compared to the control group. The number of beta cells and diameter of langerhans islets increased significantly in the rats treated by Aloe vera gel in comparison to diabetic group at the end of 15th and 30th days. Applying Aloe vera gel seems to improve the renewal and restoration of langerhans islets and beta cells of pancreas gland in the diabetic rat.

It has been hypothesized that obesity and disturbance in glucose metabolism can play an important role in the development of asthma. There is accumulating evidence in the literature that obesity can increase both the incidence and severity of asthma. Therefore, this study was conducted to determine the relationship between adiponectin, an anti-inflammatory cytokine, and blood glucose levels in overweight and obese adult patients with asthma.

Forty-fine obese or overweight men (age 38-50 years) with mild-to-moderate asthma referred to Saveh health centers participated in this study. Fasting blood glucose, insulin, and adiponectin levels were measured. The homeostasis model assessment (HOMA) was applied to evaluate beta-cell function using fasting glucose and insulin levels. Data were analyzed using Pearson correlation coefficient.

The results of this study showed that there was a significant relationship between the serum adiponectin and FEV1/FVC, FEV1 and FVC in asthma patients. In addition, serum adiponectin level was positively correlated with insulin and beta-cell function, but negatively with fasting glucose level.

According to the results, there is a negative correlation between serum adiponectin levels and blood glucose concentration in adult asthmatic patients. Furthermore, our findings showed that the reduction of systemic adiponectin level influences blood glucose concentration via insulin level and beta-cell function.