جستجوی مقالات مرتبط با کلیدواژه « Bone marrow stromal cells » در نشریات گروه « پزشکی »
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Background
It is increasingly evident that interactions between leukemic cells and their niches can have profound effects on clinical outcomes and have been contributed to the failure in treatment and drug shortage in the eradication of minimal residual disease, at least in part, through moving the cells from proliferative state to quiescent state.
ObjectivesWe, therefore, investigated the effects of different bone marrow stromal cells (BMSCs) on the induction of quiescence and tested the advantage of pan-HDAC inhibitor panobinostat in the induction of apoptosis and targeting the quiescence cells of APL-derived (NB4) and CML-derived (K562) cell lines.
MethodsWe firstly evaluated the effect of BMSCs including mesenchymal stem cell (MSC), osteoblast, and macrophage on the induction of NB4 and K562 cells quiescence in co-culture models. Next, the alterations in mRNA expression of quiescence-related genes and leukemia-driver oncogenes were evaluated in different models. Finally, the anti-leukemic effects of panobinostat were evaluated, using MTT assay and evaluation of apoptosis and G0 population.
ResultsUpon 10 days of co-culture with stromal cells, we found that leukemic cells significantly accumulated in the G0 phase. The co-cultured cells also depictured an overall overexpression of most quiescence promoter genes. The oncogenes were underexpressed in the majority of co-cultured models. The results also showed that although panobinostat could induce apoptosis in co-cultured cells, its effect on the reduction of the G0 population was more striking.
ConclusionsThese data propose that leukemia cells' quiescence state induced by stromal cells is reversible by HDAC inhibition and panobinostat could be a potentiate drug for eradication of treatment-resistance quiescence leukemic cells.
Keywords: Leukemia Niche, Macrophage, Osteoblast, Mesenchymal Stem Cells, Bone Marrow Stromal Cells, Quiescence, Panobinostat Acute Myeloid Leukemia} -
International Journal of Reproductive BioMedicine، سال هجدهم شماره 7 (پیاپی 126، July 2020)، صص 551 -560مقدمه
آپیجنین یک فلاوونویید مشتق گیاهی با اثرات آنتی اکسیدانی و آنتی آپوپتوتیک است. سلول های استرومای مغز استخوان نوعی سلول بنیادی مزانشیمال هستند که ممکن است تخمدان های آسیب دیده را بهبود دهند. بنظر می رسد آپیجنین می تواند تمایز سلول های بنیادی مزانشیمال را افزایش دهد.
هدفهدف از این مطالعه بررسی اثر تجویز همزمان آپیجنین و سلول های بنیادی استرومای مغز استخوان بر عملکرد، ساختار و آپوپتوز تخمدان های آسیب دیده بعد از ایجاد مدل شیمی درمانی با سیکلوفسفامید در موش صحرایی بود.
مواد و روش هابرای القای شیمی درمانی و تخریب تخمدان، سیکلوفسفامید به صورت داخل صفاقی به 40 موش صحرایی ماده نژاد ویستار (با وزن 200-180 گرم و سن 10 هفته) به مدت 14 روز تزریق شد. سپس موش ها به طور تصادفی به چهار گروه تقسیم شدند: کنترل، آپیجنین، سلول های بنیادی و تجویز همزمان آپیجنین و سلول های بنیادی. تزریق آپیجنین به صورت داخل صفاقی و پیوند سلول های بنیادی به صورت موضعی در تخمدان ها انجام شد. چهار هفته بعد، سطح هورمون آنتی مولرین با کیت الایزا، تعداد تخمک ها با تحریک تخمک گذاری، تعداد فولیکول ها در مراحل مختلف با رنگ آمیزی H & E و بیان پروتئین های Bcl-2 و Bax با وسترن بلات ارزیابی شد.
نتایجیافته های بدست آمده از سطح هورمون آنتی مولرین سرم، تعداد فولیکول ها و تخمک ها، و نسبت بیان Bcl-2/Bax نشان داد که تجویز همزمان آپیجنین و سلول های بنیادی استرومای مغز استخوان به طور معنا داری عملکرد، ساختار و آپوپتوز تخمدان را در مقایسه با گروه های کنترل، آپیجنین و سلول های بنیادی استرومای مغز استخوان بهبود داد (001/0p<).
نتیجه گیرینتایج پیشنهاد می کند که تجویز همزمان آپیجنین و سلول های بنیادی استرومای مغز استخوان ممکن است موثرتر از تجویز هر کدام از آن ها به تنهایی بر تخمدان آسیب دیده در اثر شیمی درمانی با سیکلوفسفامید در موش صحرایی باشد.
کلید واژگان: آپیجنین, سلول های استرومای مغز استخوان, شیمی درمانی, تخمدان, ترمیم}BackgroundApigenin is a plant-derived flavonoid with antioxidative and antiapoptotic effects. Bone marrow stromal cells (BMSCs) are a type of mesenchymal stem cells (MSCs) that may recover damaged ovaries. It seems that apigenin may promote the differentiation of MSCs.
ObjectiveThe aim of this study was to investigate the effect of coadministration of apigenin and BMSCs on the function, structure, and apoptosis of the damaged ovaries after creating a chemotherapy model with cyclophosphamide in rat.
Materials and MethodsFor chemotherapy induction and ovary destruction, cyclophosphamide was injected intraperitoneally to 40 female Wistar rats (weighing 180–200 gr, 10 wk old) for 14 days. Then, the rats were randomly divided into four groups (n = 10/each): control, apigenin, BMSCs and coadministration of apigenin and BMSCs. Injection of apigenin was performed intraperitoneally and BMSC transplantation was performed locally in the ovaries. The level of anti-mullerian hormone serum by ELISA kit, the number of oocytes by superovulation, the number of ovarian follicles in different stages by H&E staining, and the expression of ovarian Bcl-2 and Bax proteins by western blot were assessed after four wk.
ResultsThe results of serum anti-mullerian hormone level, number of oocytes and follicles, and Bcl-2/Bax expression ratio showed that coadministration of apigenin and BMSCs significantly recovered the ovarian function, structure, and apoptosis compared to the control, BMSC, and apigenin groups (p < 0.001).
ConclusionThe results suggest that the effect of coadministration of apigenin and BMSCs is maybe more effective than the effect of their administrations individually on the recovery of damaged ovaries following the chemotherapy with cyclophosphamide in rats.
Keywords: Apigenin, Bone marrow stromal cells, Chemotherapy, Ovary, Regeneration} -
ObjectiveChemotherapy may damage ovaries. L-carnitine (LC) as a type of flavonoid antioxidants and bone marrow stromal cells (BMSCs) as a type of mesenchymal stem cells may recover damaged ovaries. It seems that LC has favorable effects on differentiation, increasing lifespan and decreasing apoptosis in BMSCs. The aim of this study was to investigate the effects of co-administration of BMSCs and LC on damaged ovaries after creating a chemotherapy model with cyclophosphamide in the rat.Materials and methodsIn this experimental study, BMSCs were cultured and were confirmed by CD markers of stromal cells. Forty female Wistar rats were injected intraperitoneally with cyclophosphamide for 14 days for induction of chemotherapy and ovarian destruction. Then, the rats were randomly divided into four groups: control, LC, BMSCsthe and co-administration of LC and BMSCs. Injection of BMSCs into bilateral ovaries and intraperitoneal injection of LC were performed individually and together. Four weeks later, the levels of serum E2 and FSH using ELISA reader, the number of ovarian follicles at different stages using H&E staining, and the expression of ovarian Bcl-2 and Bax proteins using western blot were assessed.ResultsThe results showed that the effects of co-administration of BMSCs and LC were significantly more favorable than the control, BMSC and LC groups on the recovery of damaged ovaries (P<0.05).ConclusionThe effect of co-administration of BMSCs and LC is probably more effective than the effect of their separate administration on the recovery of damaged ovaries by chemotherapy.Keywords: Bone Marrow Stromal Cells, Carnitine, Chemotherapy, Ovary, Regeneration}
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IntroductionBased on our previous findings, the treatment of stem cells alone or in combination with thyroid hormone (T3) and mild exercise could effectively reduce the risk of stroke damage in young mice. However, it is unclear whether this treatment is effective in aged or middle-aged mice. Therefore, this study designed to assess whether combination of Bone Marrow Stromal Cells (BMSCs) with T3 and mild treadmill exercise can decrease stroke complications in middle-aged mice.MethodsUnder laser Doppler flowmetry monitoring, transient focal cerebral ischemia was produced by right Middle Cerebral Artery Occlusion (MCAO) for 45 min followed by 7 days of reperfusion in middle-aged mice. BMSCs (1×105) were injected into the right cerebral ventricle 24 h after MCAO, followed by daily injection of triiodothyronine (T3) (20 µg/100 g/d SC) and 6 days of running on a treadmill. Infarct size, neurological function, apoptotic cells and expression levels of Glial Fibrillary Acidic Protein (GFAP) were evaluated 1 week after stroke.ResultsPost-ischemic treatment with BMSCs or with T3 and or mild treadmill exercise alone or in combination did not significantly change neurological function, infarct size, and apoptotic cells 7 days after ischemia in middle-aged mice (P>0.05). However, the expression of GFAP significantly reduced after treatment with BMSCs and or T3 (P<0.01).ConclusionOur findings indicate that post-stroke treatment BMSCs with exercise and thyroid hormone cannot reverse neuronal damage 7 days after ischemia in middle-aged mice. These findings further support that age is an important variable in stroke treatmentKeywords: Cerebral ischemia, Combination, Bone marrow stromal cells, Thyroid hormone, Exercise, Apoptosis, Glial fibrillary acidic protein, Middle-aged, Mice}
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BackgroundThe present study assessed the alteration of gene expression during transdifferentiation of Bone Marrow Stromal Cells (BMSCs) into oligodendrocyte in the presence of Cerebrospinal Fluid (CSF).MethodsBMSCs were collected from female Sprague-Dawley rats and were cultured in DMEM/F12 medium supplemented with Retinoic Acid (RA), basic Fibroblast Growth Factor (bFGF), and Epidermal Growth Factor (EGF). CSF was added daily to the culture media. The oligoprogenitor and oligodendrocyte generation was assessed by immunocytochemistry for Oligo 2, A2B5, CNP and MBP markers.ResultsThe mean percentages of immunopositive cells for Olig2 and A2B5 were 52.1±1.74 and 56.34±2.55%, respectively. The number of immunopositive cells for glial markers CNP and MBP were 48.8±3.12 and 40.5±8.92%, respectively. Alteration of gene expression of Oct4, Olig 2, PDGFR-α and PLP were examined by real time PCR during transdifferentiation of BMSC to oligodendrocyte. Immunocytochemical results indicate that oligoprogenitor cells were immunopositive for Oligo2 and A2B5 markers. Also, oligodendrocytes expressed the mature glial markers of CNP and MBP indicating successful differentiation.ConclusionIn conclusion, CSF promotes the transdifferentiation of BMSC into mature oligodendrocyte via providing an appropriate niche for glial maturation.Keywords: Bone marrow stromal cells , Cells , Cerebrospinal fluid , Oligodendrocyte , Oligoprogenitor}
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سابقه و هدفمقاومت دارویی در لوسمی میلوئیدی مزمن، یکی از چالش ها در درمان این بیماری می باشد. در این مطالعه با هدف القای آپوپتوز در سلول های رده سلولی K562 (لوسمی میلوئیدی مزمن) و سلول های بنیادی مزانشیمی مشتق از مغز استخوان رت (rBMSCs) جهت بررسی مارکر های سطح سلولی CD33 و CD34 و هم چنین درصد آپوپتوز صورت گرفته، هم کشتی این دو رده سلولی انجام شد.مواد و روش هادر یک مطالعه تجربی، پس از جداسازی rBMSCs ، این سلول ها به لحاظ تمایز به استخوان، چربی و غضروف شناسایی و هم چنین از نظر بیان مارکر های سطحی CD90 ، CD105 ، CD45 و CD56تعیین ماهیت شدند. سپس سلول های K562 جهت بررسی مارکر های سطح سلولی CD33 و CD34 و آپوپتوز با استفاده از روش فلوسیتومتری جمع آوری گردیدند.یافته هانتایج حاصل از ایمنوفنوتایپینگ سلول ها حاکی از بیان مثبت CD90 و CD105 و بیان منفی CD45 و CD56 بوده و بیان مارکر های سطح سلولی CD33 و CD34 در رده سلولی K562 در حضور rBMSCs به ترتیب به میزان 2/45% و 5/76% افزایش یافت. القای آپوپتوز در رده سلولی K562 به طور معنادار و به میزان 6/56% در مقایسه با گروه کنترل افزایش نشان داد (05/0 p<).نتیجه گیریمطالعه حاضر نشان داد، هم کشتی رده سلولی K562 با rBMSCs سبب افزایش معنادار در میزان بیان مارکر های سطح سلولی CD33 و CD34 و القای آپوپتوز در رده سلولی K562 می شود. این تاثیر می تواند از طریق فاکتور ها و سیتوکاین های مترشحه از سلول های بنیادی مزانشیمی باشد. هر چند که ارزیابی بهتر این سیتوکاین ها از نظر نوع آن ها در مطالعه های آینده توصیه می شود.کلید واژگان: لوسمی میلوئیدی مزمن, سلول های بنیادی مزانشیمی مغز استخوان, آپوپتوز, هم کشتی}Background and ObjectivesDrug resistance is one of the main challenges in the treatment of chronic myeloid leukemia (CML). In this study, with the aim of inducing apoptosis in K562 cell line (CML), this cell line was co-cultured with rat bone marrow mesenchymal stem cells (rBMSCs). Then, the rate of apoptosis as well as cell surface markers expression of CD33 and CD34 in the K562 cells line was evaluated.
Materials and MethodsIn this experimental study, after isolating rBMSCs, the multilineag differentiation capacity of rBMSCs as well as the mesenchymal stemness of these cells was identified by evaluation of CD90, CD105, CD45, and CD56 cell surface. Then, the K562 cells were subjected for evaluation of CD33 and CD34 cell surface markers and apoptosis using a flow cytometry technique.
ResultsImmunophenotyping of the cells indicated positive expression of CD90 and CD105 and negative expression of CD45 and CD56. The expression of CD33 and CD34 cell markers in the K562 cell line was increased 45.2% and 76.5%, respectively. Also, induction of apoptosis in the K562 cell line significantly increased (56.6%) compared to the control (p < 0.05).
ConclusionsBriefly, this study showed that the co-culturing of the K562 cells line with rBMSCs causes significant increase in the expression of CD33 and CD34 cell surface markers as well as induction of apoptosis in the K562 cells (p < 0.05). This effect can be through the secreted factors and cytokines of rBMSCs; however, better evaluation in terms of type of cytokines is recommended in the future studiesKeywords: Myeloid Leukemia‘Chronic, Bone Marrow Stromal Cells, Apoptosis, Co-culture} -
Bone marrow stromal stem cells (BMSCs) play a significant role in cell therapy. These cells quickly die after transplantation to the affected area due to oxidative stress. The natural disaccharide, trehalose which can be known as autophagy inducer. The present study aimed to investigate the role of trehalose in preventing BMSCs from oxidative stress caused by H2O2.
BMSCs were isolated from the adult rats. The cells were divided into three groups: (a) control; (b) 100 µM H2O2; (c) 100 µM H2O2 and trehalose 3%. The morality rate was analyzed by viability test. Immunocytochemistry and Western blot was used in order to evaluate p62 protein and LC3II/LC3I ratio, respectively. In order to evaluate apoptosis, cleaved caspase-3 protein was used. In viability test, the survival rate for BMSCs after 8 h were 82%, 72%, 49%, and 39% (for groups who received 50, 100, 200, and 400 µM H2O2, respectively) compared to the control group. Pre-treatment with the use of trehalose 3% increased cell survivals. The levels of p62 protein, were increased in the cells under H2O2 treatment, while the levels of p62 protein in the cytoplasm, as autophagy inclusions, reduced for the group with trehalose pre-treatment. In addition, trehalose caused to increase LC3II/LC3I ratio and decreased the expression of cleaved caspase-3.Trehalose decreased apoptosis and increased the autophagy and survival levels of the cells against H2O2. Due to the unique properties of trehalose and its low toxicity, it can be used as a pharmaceutical agent in cellular transplantation to reduce oxidative stress.Keywords: Stress oxidative, Autophagy, Apoptosis, Bone marrow stromal cells, Trehalose} -
ObjectiveGhrelin is a peptide which has a proliferative and antiapoptotic effect in many cells including bone marrow stromal cells (BMSCs). Homeobox protein B4 (HOXB4) is a transcription factor involved in stem cell regeneration and survival. The aim of the study was to find out the efect of ghrelin on Hoxb4 expression in BMSCs.Materials And MethodsIn this experimental study, rat BMSCs were cultivated in Dulbeccos Modified Eagle Medium (DMEM). Passage three BMSCs were treated with ghrelin 100 μM for 48 hours. Real-time polymerase chain reaction (PCR) was carried out from the untreated BMSCs (B), BMSCs treated with 125 μM H2O2 (BH), BMSCs treated with 100 μM ghrelin then 125 μM H2O2 (BGH) and BMSCs treated with 100 μM ghrelin (BG) groups. For immunofluorescence, cells were incubated with an anti-HOXB4 monoclonal antibody. Primary antibodies were visualized using the Fluorescein isothiocyanate (FITC) method. All data are presented as mean ± SEM and PResultsHoxb4 expression significantly increased in the BG compared with BH and BGH groups. Furthermore, 100 μM ghrelin, increased the mean of HOXB4 positive immunoreactive cells compared to the BH group.ConclusionGhrelin probably enhances proliferation and viability of BMSCs through Hoxb4 upregulation. However, the signaling pathway and other biological outcomes of this effect should be elucidated in different stem cells.Keywords: Bone Marrow Stromal Cells, Ghrelin, HOXB4, Rat}
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ObjectiveAlthough stem cell transplantation has beneficial effects on tissue regeneration, but there are still problems such as high cost and safety issues. Since stem cell therapy is largely dependent on paracrine activity, in this study, utilization of transplantation of bone marrow stromal cells (BMSCs)-secretome instead of the cells, into damaged ovaries was evaluated to overcome the limitations of stem cell transplantation.Materials And MethodsIn this experimental study, BMSCs were cultured and 25-fold concentrated conditioned medium (CM) from BMSCs was prepared. Female rats were injected intraperitoneally with cyclophosphamide (CTX) for 14 days. Then, BMSCs and CM were individually transplanted into bilateral ovaries, and the ovaries were excised after four weeks of treatment. The follicle count was performed using hematoxylin and eosin (H&E) staining and the apoptotic cells were counted using TUNEL assay. Ovarian function was evaluated by monitoring the ability of ovulation and the levels of serum estradiol (E2) and follicle-stimulating hormone (FSH).ResultsEvaluation of the ovarian function and structure showed that results of secretome transplantation were almost similar to those of BMSCs transplantation and there was no significant differences between them.ConclusionBMSCs-secretome is likely responsible for the therapeutic paracrine effect of BMSCs. Stem cell- secretome is expected to overcome the limitations of stem cell transplantation and become the basis of a novel therapy for ovarian damage.Keywords: Bone Marrow Stromal Cells, Chemotherapy, Conditioned Medium, Ovary, Transplantation}
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Objective(s)To study the effect of low-frequency vibration on bone marrow stromal cell differentiation and potential bone repair in vivo.Materials And MethodsForty New Zealand rabbits were randomly divided into five groups with eight rabbits in each group. For each group, bone defects were generated in the left humerus of four rabbits, and in the right humerus of the other four rabbits. To test differentiation, bones were isolated and demineralized, supplemented with bone marrow stromal cells, and implanted into humerus bone defects. Varying frequencies of vibration (0, 12.5, 25, 50, and 100 Hz) were applied to each group for 30 min each day for four weeks. When the bone defects integrated, they were then removed for histological examination. mRNA transcript levels of runt-related transcription factor 2, osteoprotegerin, receptor activator of nuclear factor k-B ligan, and pre-collagen type 1 a were measured.ResultsHumeri implanted with bone marrow stromal cells displayed elevated callus levels and wider, more prevalent, and denser trabeculae following treatment at 25 and 50 Hz. The mRNA levels of runt-related transcription factor 2, osteoprotegerin, receptor activator of nuclear factor k-B ligand, and pre-collagen type 1 a were also markedly higher following 25 and 50 Hz treatment.ConclusionLow frequency (2550 Hz) vibration in vivo can promote bone marrow stromal cell differentiation and repair bone injury.Keywords: Bone injury, Bone marrow stromal cells, Pre, Col1a, RUNX2, Vibration stress}
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ObjectiveBone marrow and umbilical cord stromal cells are multipotential stem cells that have the ability to produce growth factors that play an important role in survival and generation of axons. The goal of this study was to evaluate the effects of the two different mesenchymal stem cells on peripheral nerve regeneration.Materials And MethodsIn this experimental study, a 10 mm segment of the left sciatic nerve of male Wistar rats (250-300 g) was removed with a silicone tube interposed into this nerve gap. Bone marrow stromal cells (BMSCs) and human umbilical cord stromal cells (HUCSCs) were respectively obtained from rat and human. The cells were separately cultured and transplanted into the nerve gap. The sciatic nerve regeneration was evaluated by immunohistochemistry, and light and electron microscopy. Moreover, histomorphology of the gastrocnemius muscle was observed.ResultsThe nerve regeneration in the BMSCs and HUCSCs groups that had received the stem cells was significantly more favorable than the control group. In addition, the BMSCs group was significantly more favorable than the HUCSCs group (P<0.05).ConclusionThe results of this study suggest that both homograft BMSCs and heterograft HUCSCs may have the potential to regenerate peripheral nerve injury and transplantation of BMSCs may be more effective than HUCSCs in rat.Keywords: Bone Marrow Stromal Cells, Human Umbilical Cord Stromal Cells, Transplantation, Peripheral Nerve, Regeneration}
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IntroductionSOX9 is a transcriptional activator which is necessary for chondrogenesis. SOX6 are closely related to DNA-binding proteins that critically enhance its function. Therefore, to carry out the growth plate chondrocyte differentiation program, SOX9 and SOX6 collaborate genomewide. Chondrocyte differentiation is also known to be promoted by glucocorticoids through unknown molecular mechanisms.MethodsWe investigated the effects of asynthetic glucocorticoid, dexamethasone (DEX), on SOX9 gene expression in chondrocytes.ResultsSOX9 mRNA was expressed at high levels in these chondrocytes. Treatment with DEX resulted in enhancement of SOX9 mRNA expression. The DEX effect was dose dependent (0·5 nM and 1 nM).ConclusionRT-PCR analysis revealed that DEX also enhanced the levels of SOX9 expression. It was observed that DEX had enhancing effect only on SOX9 the expression level was low for SOX6. It can thus be concluded that chondrocyte differentiation can be promoted by DEX via SOX9 enhancement.Keywords: SOX6, SOX9, Bone marrow stromal cells, Differentiation}
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Objective (s): Intracerebral injection of bone marrow stromal cells (BMSCs) is being investigated as a therapeutic tool to prevent Alzheimer''s disease (AD). Our aim was to investigate the effects of BMSCs by intrathecal injection in AD rat model.Materials And MethodsBMSCs were obtained from the bone marrow of Wistar rat and transplanted into AD rat model via intrathecal injection. The rat model had received an injection of β amyloid into the hippocampus for histological and immunohistochemical studies.ResultsHistological examination of the brains in transplanted rats compared to controls demonstrated the migration of BrdU-labeled BMSCs from the site of delivery، confirmed the differentiation of BMSCs transplanted cells into the cholinergic neurons، and increased number of healthy and decreased number of dark neurons.ConclusionOur results showed that BMSCs intratechal administration could be a promising method for treatment ofAlzheimer’s disease in rat model.Keywords: Alzheimer's disease, Bone marrow stromal cells, Intrathecal delivery, Rat model}
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مقدمه و هدفیکی از راه های درمان بیماری های با نقص میلین، سلول درمانی است؛ سلول های بنیادی مورد استفاده در این روش به الیگودندروسیت تبدیل خواهندشد. در مطالعات پیشین برای تولید سلول های شبه الیگودندروسیت از سلول های بنیادی مغز استخوان استفاده می شد که در مرحله پیش القا تحت تاثیرDMSO و در مرحله القا، تحت تاثیر PDGF، bFGF، heregulin and T3 قرارمی گرفتند. در تحقیق اخیر به منظور افزایش تولید این سلول ها ابتدا سلول های بنیادی مغز استخوان به نوروسفر و سلول های بنیادی عصبی تبدیل شده، سپس سلول، شبه الیگودندروسیت شد و در پایان، همکشتی این سلول ها با نورون های حرکتی به منظور تولید میلین ارزیابی شد.مواد و روش هاسلول های بنیادی مغز استخوان (BMSCs) از موش های صحرایی ماده بالغ استخراج و تحت اثر B27، EG وBFGF به سلول های نوروسفر(NSF) و سلول های بنیادی عصبی (NSC) تبدیل و سپس در مرحله القا، تحت تاثیر (PDGF، bFGF، heregulin and T3). به سلول های شبه الیگودندروسیت (OLCs) تمایزداده شدند. سلول های سلول های OLC، NSF، NSC و BMSC با استفاده از نشانگرهای (مارکرهای) CD44، CD45 CD90، fibronectin و oct-4، ، MBP O1، O4، GFAP مورد ارزیابی ایمونوسایتوشیمیایی و ژن های oligo2و MOG با RT-PCR مورد بررسی قرارگرفتند.نتایجنتایج مطالعه حاضر، نشان دهنده بیان مارکرهای C D44، CD45، CD90 fibronectin توسط سلول های BMSCs و مارکرهای NESTIN وNF68 توسط سلول های NSC و مارکرهای O1، O4، oligo2 در سلول های OLCs بود؛ درضمن، بیان ژن های Olig2 و PDGFR توسط روش RT-PCR تاییدشد.نتیجه گیریسلول های شبه الیگودندروسیت با درصدی بالا از سلول های بنیادی عصبی استحصال یافته از BMSCs ایجاد می شوند و پس از همکشتی با نورون های حرکتی، قابلیت تولید میلین در محیط کشت را دارا هستند.
کلید واژگان: سلول های شبه الیگودندروسیت, نورون های حرکتی, سلول های بنیادی مشتق از مغز استخوان, القا, سلول های بنیادی عصبی}Background And ObjectiveOne of the approaches for treatment of demylination diseases is cell therapy. Stem cells have been used as a source for generating oligodendrocyte. In a previous communication، the oligodendrocyte-like cells (OLCs) were generated from bone marrow stromal cells (BMSCs) using preinducers (dimethyl sulfoxide and retinoic acid) and induction protocol (PDGF، bFGF، heregulin and T3). In this investigation، we tried to increase the yield of oligodendrocyte-like cells (OLCs) by inducing the neural stem cells generated from neurospheres-derived BMSCs.Materials And MethodsIn this research study، BMSCs of adult female rats were expanded and then induced to form neurospheres (NS) using B27، the epidermal growth factor (EGF) and the basic fibroblast growth factor (bFGF)، and subsequent isolation of neural stem cells (NSCs). This was followed by induction of the NSCs into OLCs with heregulin، PDGF-AA، bFGF and T3. BMSCs، NS، NSCs and OLCs were characterized using immunocytochemistry for fibronectin، CD44، CD90، CD45، Oct-4، O4، Oligo2، O1 and MBP markers. PDGF receptor α (PDGFR-α)، Olig2 and MOG expression were evaluated using RT-PCR.ResultsThe BMSCs expressed CD44، CD90، CD106 and Oct-4; the NSCs were immunoreactive to nestin and NF68، while the OLCs were immunoreactive to O4، Oligo2 and O1. In differentiating OLCs، Olig2 and PDGFR-α mRNA was detected using RT-PCR technique.ConclusionFunctional OLCs were generated from BMSCs-derived NS with high yield and were able to produce myelin after co-culture by motor neurons.Keywords: Oligodendrocyte, like cells, Bone marrow stromal cells, Motoneurons, Induction, Neural stem cells} -
زمینه و هدفسلول های بنیادی بافت چربی از نظر ظاهری بسیار شبیه به سلول های استرومایی مغز استخوان هستند و میتوانند جایگزینی مناسب برای این سلول ها باشند. هدف از این مطالعه بررسی و مقایسه درصد حیات و تمایز خود به خودی سلول های بنیادی بافت چربی و سلول های استرومایی مغز استخوان در پاساژهای طولانی مدت می باشد.مواد و روش هادر این مطالعه آزمایشگاهی ابتدا سلول های بنیادی بافت چربی و مغز استخوان از موش های صحرایی تهیه شد و سپس در پاساژهای مختلف درصد سلول های مرده با روش (live and dead cells assay) و توانایی تمایز خود به خودی سلول ها به سلول های چربی ساز و استخوان ساز به ترتیب با استفاده از رنگ آمیزی Oil Red O و Alizarin red بررسی شد. اطلاعات به دست آمده توسط Student t-test و روش آماری آنالیز واریانس یک طرفه (ANOVA) بررسی گردید.یافته هانتایج به دست آمده از این مطالعه نشان داد میانگین درصد سلول های مرده در پاساژهای پنجم، دهم و پانزدهم برای سلول های استرومایی مغز استخوان به ترتیب (36/0±26/2، 45/0±39/3 و 95/0±92/6) و برای سلول های بنیادی بافت چربی به ترتیب (19/0±7/0، 39/0±89/0 و 55/0±23/3) بدست آمد. همچنین، در بررسی تمایز خود به خودی به سلول های استخوان ساز نتایج بدست آمده در پاساژ پانزدهم برای سلول های استرومایی مغز استخوان (38/0±25/3) و برای سلول های بنیادی بافت چربی (3/0 ± 48/0) بود، که این نتایج دارای اختلاف معنی دار بودند (05/0>p). همچنین، در بررسی تمایز سلول ها به سلول های چربی ساز نتایج نشان داد تا پاساژ 15 سلول های بنیادی بافت چربی توانایی تمایز خود به خودی به سلول های چربی ساز را ندارند ولی سلول های مغز استخوان در پاساژ پانزدهم به میزان 24/0 ± 07/1 تمایز را نشان دادند.نتیجه گیریبا توجه به درصد سلول های مرده و تمایز خودبه خودی در پاساژهای طولانی مدت، سلول های بنیادی بافت چربی می توانند منبع جایگزین مناسبی برای سلول های بنیادی مغز استخوان به منظور کاربرد های درمانی باشند.
کلید واژگان: سلول بنیادی بافت چربی, سلول های بنیادی مغز استخوان, تمایز خود به خودی, حیات سلول ها}Background And ObjectiveAdipose tissue-derived stem cells (ADSCs) are very similar in their cell properties to bone marrow stromal cells (BMSCs) and can be a suitable alternative for cell therapy trials. The purpose of this study is comparison of adipose-derived stem cells (ADSCs) and bone marrow stromal cells (BMSCs) in prolonged passages based on viability and auto-differentiation toward adipocytes and osteocytes, respectively.Materials And MethodsIn this experimental study, stem cells were harvested from adipose tissue and bone marrow, then, at different cell passages the percentage of dead cells was assessed by live and dead cells assay and spontaneous differentiation toward adipocytes and osteocytes evaluated using Oil Red O and Alizarin red staining, respectively. T-test and one-way ANOVA statistical analysis was used for assessment of obtained data.ResultsAttained results of this study showed that the mean percentage of death cells during 5th, 10th and 15th for BMSCs were (2.26±0.36, 3.39±0.45, 6.92±0.95) and for ADSCs were (0.7±0.19, 0.89±0.39, 3.23±0.55), respectively. Also spontaneous differentiation into bone-forming cells of ADSCs and BMSCs in 15th passage shown significant difference for BMSCs (3.25± 0.38) and ADSCs (0.48±0.3) (p< 0.05). Our data show that ADSCs did not spontaneously adipogenic differentiate during 15th passage, but adipogenic differentiation for BMSCs was positive (1.07±0.24).ConclusionConsidering of the percentage of dead cells and spontaneous differentiation with long passages revealed that ADSCs can be a suitable alternative source for BMSCs in therapeutic applications.Keywords: Adipose tissue, derived stem cells, Bone marrow stromal cells, Spontaneous differentiation, Survival cells} -
مجله دانشکده پزشکی دانشگاه علوم پزشکی تهران، سال هفتاد و دوم شماره 7 (پیاپی 163، مهر 1393)، صص 449 -456زمینه و هدفسلول های استرومال مغز استخوان رهیافت های جدیدی را در پیش روی مدیریت درمان آسیب های شدید پوستی قرار داده است. این سلول ها شامل جمعیت هتروژنی از سلول های بنیادی مزانشیمال، خونساز و فیبروبلاست می باشند که از طریق تولید فاکتورهای رشد و تمایز به سلول های رده مزودرمال می توانند در درمان آسیب های بافتی مورد استفاده قرار گیرند. هدف از مطالعه حاضر، بررسی تاثیرات تزریق زیر جلدی سلول های استرومال مغز استخوان، در التیام سوختگی پوستی درجه سه در موش سوری بود.روش بررسیدر یک مطالعه تجربی که در پژوهشکده دانشگاه ارومیه از دی 1390 تا تیر 1391 انجام پذیرفت، 18 سر موش سوری نر با محدوده سنی 8-7 هفته تحت القای سوختگی درجه سه در ناحیه پشت قرار گرفتند. به دنبال تقسیم بندی تصادفی موش ها در دو گروه کنترل و درمان سلولی، یک ساعت پس از اعمال سوختگی، موش ها به ترتیب تحت تزریق زیر جلدی در ناحیه سوختگی با بافر فسفات سالین و سلول های استرومال به تعداد یک میلیون سلول در حجم lμ 400 بافر فسفات سالین قرار گرفتند. با تهیه مقاطع بافتی در روزهای 7، 14 و 21 پس از القای سوختگی، رنگ آمیزی بافتی با روش های هماتوکسیلین- ائوزین و ماسون تری کروم انجام پذیرفت.یافته هابه لحاظ پارامترهای بررسی شده شامل شکل گیری بافت جوانه ای (به ترتیب در روزهای 7، 14 و 21، 007/0P≤، 013/0P≤ و 001/0P≤)، رگ زایی (روز 21، 002/0P≤) و رسوب کلاژن نتایج نشان دادند که در گروه درمان شده با سلول، سرعت روند التیام به طور معناداری بیشتر از گروه کنترل بود (05/0P≤).نتیجه گیریسلول های استرومال مغز استخوان می توانند از طریق تحریک شکل گیری بافت جوانه ای، رگ زایی و تکثیر فیبروبلاستی به همراه رسوب بیشتر کلاژن در التیام آسیب پوستی ناشی از سوختگی موثر واقع شوند.
کلید واژگان: سوختگی, التیام بافت, رت, سلول استرومال مغز استخوان}BackgroundRecently، bone-marrow-derived cells have introduced new therapeutic approaches to the management of wound healing in severe skin injuries. Bone marrow-derived stromal cells are described as a heterogeneous population، including mesenchymal stem cells، hematopoietic stem cells، and fibro-blast cells. Results derived from several studies indicate that these cells may contribute to tissue regeneration whether through producing variety of bioactive growth factors and/or by differentiation into mesoderm lineage. The aim of the present study was to investigate the effect of subcutaneous administration of bone marrow-derived stromal cells in repairing or regeneration of skin wounds induced by third-degree burn in a mouse model.MethodsIn an experimental study that was performed in Urmia University research center from December 2011 to June 2012، The third-degree skin burn was induced on the shaved backs of healthy 7-8 week old male mice (N=18) using a metal rods heated in boiling water. After 1 hour، based on the equal physical condition mice were randomly divided into two separate groups and then subcutaneously administered with phosphate buffered saline (PBS; 400 µl) or bone marrow-derived stromal cells (106 cell in 400µl PBS) at the burn site. 7، 14 and 21 days after induction of burn injury، biopsies were taken from burn wounds and then the sections were prepared. Subsequently the prepared sections were stained with hematoxylin/eosin and Masson''s trichrome to explore histopathological changes evoke by administration of bone marrow derived stromal cells in comparison with control subjects.ResultsConsidering investigated parameters including formation of granulation tissue (respectively on days 7، 14 and 21 P≤ 0/007، P≤ 0/0013 and P≤ 0/001)، angiogenesis (on day 21 P≤ 0/002) and collagen deposition، in mice treated with bone marrow-derived stromal cells the rate of healing of third-degree thermal burns was significantly accelerated when compared to the PBS-treated mice.ConclusionThis experimental modulation of wound healing suggests that bone marrow-derived stromal cells can significantly enhance the rate of wound healing possibly through stimulation of granulation tissue، angiogenesis، fibroblast proliferation and collagen deposition.Keywords: bone marrow stromal cells, burns, rat, tissue regeneration} -
پیش زمینه و هدفایسکمی مغزی به عنوان یک معضل بزرگ جهانی شناخته شده است و ریپرفیوژن متعاقب آن درنهایت منجر به مرگ برنامه ریزی شده سلولی یا آپوپتوز می گردد. نواحی مشخصی از مغز و انواع خاصی از نورون ها ازجمله نورون های هرمی ناحیه CA1 هیپوکامپ نسبت به ایسکمی مغزی حساس ترند. یکی از روش های درمانی که امروزه به طور شایع در بین محققین رواج دارد سلول درمانی (cell therapy) می باشد. لذا در این مطالعه برآن شدیم تا میزان آپوپتوز در سلول های هیپوکامپ را پس از تزریق داخل وریدی سلول های بنیادی مزانشیمی مغز استخوان (BMSCs) در مدل ایسکمی- ریپرفیوژن موردبررسی قرار دهیم.مواد و روش هادر این مطالعه 40 سر موش صحرایی نر بالغ با وزن 250-300 گرم به طور تصادفی به پنج گروه هشت تایی تقسیم شدند که شامل گروه های کنترل، شم، ایسکمی، vehicle،treatment بودند گروه شم متحمل استرس جراحی بدون مسدود کردن شریان های کاروتید مشترک شد. در گروه ایسکمی شریان های کاروتید مشترک هردو طرف به مدت بیست دقیقه مسدود شده و سپس مجددا جریان خون برقرار شد. در گروه vehicle ایسکمی ایجادشده و پس از 7 روز 30μl PBS از طریق ورید دمی تزریق شد. در گروه treatment ایسکمی ایجاد شد و پس از 7 روز سلول های BMSC به تعداد 800 هزار در 30μl سوسپانسیون سلولی از طریق ورید دمی تزریق شد.72 ساعت قبل از تزریق سلول ها با Brdu نشان دار شدند. در روز 12 پس از ایسکمی با روش پرفیوژن داخل قلبی و با پارافرمالدئید 4 درصد موش ها فیکس شدند و مغز آن ها خارج گردید و پس از پردازش بافتی برش های 5 میکرونی تهیه شد. جهت شناسایی سلول های BMSC بررسی ایمونوهیستوشیمی با آنتی بادی Anti-Brdu انجام شد. برای نشان دادن سلول های آپوپتوتیک از رنگ آمیزی تانل استفاده شد.یافته هادر نمونه های ایسکمی ایندکس آپوپتوز 43/37 درصد مشاهده شد که این میزان در گروه درمان 23/61 درصد بود این یافته ها نشان می دهند که میزان آپوپتوز در گروه تحت درمان با سلول های بنیادی مغز استخوان در مقایسه با گروه ایسکمی بسیار کمتر بودنتیجه گیریایسکمی- ریپرفیوژن به مدت 20 دقیقه باعث آسیب و مرگ نورونی وسیع در هیپوکامپ به ویژه در ناحیه CA1 می شود و تزریق سلول های بنیادی مغز استخوان به طور معنی داری موجب کاهش تعداد نورون های آپوپتوتیک می گردد.
کلید واژگان: ایسکمی, ریپرفیوژن, هیپوکامپ, سلول های بنیادی مزانشیمی مغز استخوان, آپوپتوز}Background and AimsCerebral ischemia is known as a major worldwide problem and subsequent reperfusion leads to apoptosis or programmed cell death. Specific regions of the brain and specific types of neurons, including hippocampal CA1 pyramidal neurons are more sensitive in cerebral ischemia. Today cell therapy is one of the common treatments that spread among the researchers. In this study, we evaluated apoptosis in hippocampal cells of rat following intravenous injection of bone marrow stromal cells (BMSCs) in ischemia-reperfusion model.Materials and MethodsIn this study, adult male wistar rats (n=40) weighting (250-300g) were used. The rats were divided into the five groups of 8 animals including: control, sham, ischemia, vehicle, and treatment. In the sham group surgery was performed without blocking common carotid arteries. In ischemia group common carotid arteries blocked for twenty minutes and then allowed to reperfusion. In the vehicle group 7 days after ischemia, 30µl PBS was injected via tail vein. In the treatment group BMSC cells (800000/ 30 µl suspension) were injected into the tail vein 7 days after ischemia. And 72 hours before transplantation, the cells were labeled with Brdu. 12 days after ischemia, rats were fixed with 4% Paraformaldehyde through transcardial perfusion and their brains were removed. After histological processing, 5 micron sections were prepared for staining. For immunohistochemistry study anti-Brdu anti-body was used and BMSC cells were identified. Apoptotic cells were detected by TUNEL staining.ResultsSever apoptosis was observed in ischemia group. The mortality rate in the group which was treated with BMSC was lower.ConclusionIschemia-reperfusion for 20 minutes causes damage and extensive neural death in the hippocampus, especially in CA1 region and injection of bone marrow stromal stem cells significantly decreased the number of apoptotic neurons.Keywords: Ischemia, Reperfusion, Hippocampal, Bone marrow stromal cells, Apoptosis} -
سابقه و هدفمهم ترین فاکتور در انتخاب پلیمر جهت تهیه نانو فیبر برای استفاده در مقاصد پزشکی و بیولوژی ویژگی های زیست سازگاری و زیست تخریب پذیری است. پلیمر پلی لاکتیدکوپلی گلیکولید (poly (lactic-co-glycolic acidPLGA)) بخاطر مزایای فوق و نیز توجه بسیار در امر مهندسی بافت، در تحقیق حاضر که هدف BMSCs موش صحرایی رت آن بررسی کشت سلول های استرومایی مغز استخوان بر روی نانو الیاف PLGA است.مواد و روش هادر این مطالعه ابتدا به روش الکتروریسی نانو الیاف PLGA در حلال هگزا فلورو بیس پروپانول تهیه شد. ویژگی های این پلیمر با روش میکروسکوپ الکترونی روبشی و میکروسکوپ اینورت مورد بررسی قرار گرفت. پس از استحصال سلول ها و رسیدن به پاساژ دو، سلول ها دردو گروه روی بستر بدون نانو الیاف و بستر حاوی نانو الیاف PLGA کشت داده شدند. حیات و مرگ سلول ها در روزهای دوم، چهارم و ششم توسط رنگ آمیزی اکریدین اورنج و مورفولوژی سلول ها روی بستر نانو الیاف به وسیله میکروسکوپ الکترونی روبشی مورد بررسی قرار گرفت.یافته هانتایج رشد سلول ها روی بستر نانو الیاف PLGA در مقایسه با رشد سلول در محیط کشت از لحاظ میزان رشد و مورفولوژی اختلافی نداشتند.استنتاجبا توجه به بررسی نتایج حاصل ازکشت سلول های مغز استخوان می توان نتیجه گیری کرد که از نانو الیاف PLGA می توان به عنوان داربستی زیست تخریب پذیر و زیست سازگار به همراه سلول های مغز استخوان در امر مهندسی بافت بهره گرفت.
کلید واژگان: سلول های استرومایی مغز استخوان, مهندسی بافت, کشت سلول, PLGA نانوالیاف}Background andPurposeProperties of biocompatibility and biodegradation are important factors in choosing a polymer to prepare nano-fiber for medical and biological purposes. PLGA high attention in tissue engineering for the above properties، the aim of present study is the evaluation of rat bone marrow stromal cells cultured on PLGA nanofibers.Material And MethodsIn this study، the PLGA nanofibers were prepared using electrospinning technique in hexa fluoro propanol solvent. To study the nanofiber properties scanning electron microscopy (SEM) and invert microscope were used. After bone marrow stromal cells culture (BMSCs) and achieving passage two، the cells were grown in two groups of PLGA nanofibers and without nanofibers. Life and death of cells were examined in second، fourth، and sixth days by Acridine Orange. Also، the morphology of cells was investigated via light microscopy.ResultsThe results showed no significant reduction in cell growth in PLGA nanofibers compared with that of the control group.ConclusionAccording to the results obtained from BMSCc culture، PLGA nanofibers could be used as biodegradable and biocompatible scaffold alongside bone marrow cells in tissue engineering.Keywords: Nano fiber, PLGA, bone marrow stromal cells, tissue engineering, cell culture} -
IntroductionTransplantation of bone marrow stromal cells (BMSCs) or Schwann cells (SCs) can increase axonal regeneration in peripheral nerve injuries. Based on our previous investigations, the goal of the present work was to examine the individual and synergistic effects of the two different cell types in sciatic nerve injury. We pursued to evaluate the effects of BMSCs and SCs co-transplantation on the functional recovery after sciatic nerve injury in rat.MethodsIn this experimental research, adult male Wistar rats (n=32, 250-300g) were used, BMSCs and SCs were cultured, and the SCs were confirmed with anti S100 antibody. Rats were randomly divided into 4 groups (n=8 in each group): 1- control group: silicon tube filled with fibrin gel without cells; 2- BMSCs group: silicon tube filled with fibrin gel seeded with BMSCs; 3- SCs group: silicon tube filled with fibrin gel seeded with SCs and 4- co-transplantation group: silicone tube filled with fibrin gel seeded with BMSCs and SCs. The left sciatic nerve was exposed, a 10 mm segment removed, and a silicone tube interposed into this nerve gap. BMSCs and SCs were transplanted separately or in combination into the gap. BMSCs were labeled with anti-BrdU and SCs were labeled with DiI. After 12 weeks electromyographic and functional assessments were performed and analyzed by one-way analysis of variance (ANOVA).ResultsElectromyographic and functional assessments showed a significant difference between the experimental groups and controls. Electromyography measures were significantly more favourable in SCs transplantation group as compared to BMSCs transplantation and co-transplantation groups (p<0.05). Functional assessments showed no statistically significant difference among the BMSCs, SCs and co-transplantation groups (p<0.05).DiscussionTransplantation of BMSCs and SCs separately or in combination have the potential to generate functional recovery after sciatic nerve injury in rat. The electromyography evaluation showed a greater improvement after SCs transplantation than BMSCs or the co-transplantation of BMSCs and SCs.Keywords: Bone Marrow Stromal Cells, Schwann Cells, Transplantation, Peripheral Nerve Regeneration}
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مقدمه و هدفمطالعات متعددی در زمینه دستیابی به داربستی جهت رشد سلولهای بنیادی روی آن انجام شده است. هدف از این تحقیق ارائه داربست زیست تخریب پذیر کیتوسان-پلی اتیلن اکسید (PEO) با بررسی توانایی رشد، تکثیر، عدم تمایز و مرگ سلولی سلولهای استرومایی مغز استخوان (BMSCs) بر روی آن می باشد.مواد و روش هاابتدا تشکیل نانوالیاف کیتوسان-PEO به نسبت90 به 10و80 به 20 به روش الکتروریسی، توسط میکروسکوپ الکترونی روبشی (SEM) مورد بررسی قرار گرفت. این اسکافولد بر روی ژلاتین1% در پلیت24خانه ای قرار گرفته و استریل شد. سلولهای BMSCs از استخوان ران موش های صحرایی بالغ استخراج و پس از سه مرحله پاساژ در خانه های خالی پلیت به عنوان گروه شاهد و هم بر روی داربست کشت داده شد. میزان تکثیر، عدم تمایز و سلولهای در حال مرگ در طی روزهای دوم، چهارم و ششم مورد بررسی قرار گرفت.نتایجنتایج نشان داد که مورفولوژی سلول ها بر روی داربست حفظ شده و مشابه گروه شاهد بود. میزان تکثیر سلولها روی داربست در روزهای متوالی افزایش یافته و اختلاف معنی داری با میزان تکثیر سلولها در کشت سلولها در گروه شاهد نداشت. یافته ها همچنین نشان داد درصد تمایز سلول های BMSCs و درصد میزان مرگ سلولی نیز در سلول های کشت داده شده روی داربست در مواجه با داربست در پایان روز ششم مشابه گروه شاهد بود.نتیجه گیریتکثیر، عدم تمایز و عدم مرگ سلولی BMSCs بر روی نانوالیاف کیتوسان-PEO زیست تخریب پذیر منجر به ارایه مدلی از داربست گردید، که می تواند در مهندسی بافت و سلول درمانی مورد استفاده قرار گیرد.
کلید واژگان: نانوالیاف, کیتوسان, داربست, BMSCs}Background And ObjectiveSeveral studies have been performed to achieve a scaffold for growing stem cells. The purpose of the study was to provide a biodegradable scaffold of chitosan - poly ethylene oxide (PEO) with the ability for growing, proliferation, un-differentiation and apoptosis of bone marrow stromal cells (BMSCs).Materials And MethodsFirst, formation of chitosan-PEO nanofibers composed of 90 to 10 and 80 to 20 per electro technique were studied by scanning electron microscope (SEM). These scaffolds were located on 1% gelatin in 24-well plates and were then steriled. Femoral BMSCs of rats were cultured on scaffolds after two passages from the house empty plate as controls. BMSCs proliferation, differentiation and apoptosis were studied in days II, IV and VI.ResultsThe results showed that the morphology of cells was maintained on scaffolds similar to controls. The rate of cell proliferation on the scaffold on consecutive days increased in cultured cells of control group but the differences were not significant. The results also showed that at the end of the six days, BMSCs differentiation and the percentage of cell death on the scaffold were similar with cultured cells in control group.ConclusionProliferation, un-differentiation and no apoptosis of BMSCs on biodegradable chitosan-PEO nanofiber are obtained as a model that can be used in tissue engineering and cell therapyKeywords: Nanofibers, Chitosan, Scaffolds, Bone Marrow Stromal Cells}
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