Evaluation of anaerobic pathogens in periodontitis patients and its relationship with TGF-1β genomic polymorphism by Tetra Arms-PCR method

Periodontitis is a common and inflammatory infectious disease that causes damage to the tissues supporting the tooth and consequent tooth loss. Periodontal disease is a multimicrobial and multifactorial disease and important anaerobic bacteria are involved in periodontal infection. TGF-1β is one of the growth factors and anti-inflammatory cytokines that play a crucial role in the repair of periodontal lesions. The aim of this study was to evaluate Tetra Arms-PCR with high sensitivity and specificity, which can be used to evaluate the genomic polymorphism among oral samples and show the relationship between TGF-1β and periodontal disease.

The present study was a case-control study in the periodontology department of Kerman Dental School. Sampling was done from 100 samples including 50 healthy individuals and 50 patients with microbial periodontal infection. Genotype was analyzed using DNA extracted from the blood of patients by PCR -ARMs-Tetra to determine the relationship between TGF-1β genomic polymorphism and periodontitis. Statistical analysis was performed with SPSS19 software and one-way ANOVA.

The samples were culture positive, therefore, more than 65% of the isolated bacteria were anaerobic which included: Prevotella, Porphyromonas, Fusobacterium, Peptostreptococcus anaerobic. The results of Tetra PCR ARMs after sequence frequency were genotype CC allele (25%), CT allele (20%), TT allele (5%). Percentage of control group were CC allele (20%), CT allele (24%), and TT allele (6%). The frequency of C and T alleles in the patient group was 70% and 30%, and in the control group 63% and 37%, respectively with no significant difference between two groups (P=0.83).

According to the results of this study and the application of anaerobic conditions, forced anaerobic bacteria can be isolated from clinical specimens of oral infections and by Tetra Arms-PCR no significant relationship between TGF-1β genomic polymorphism and periodontitis was observed. In addition, therer was no significant difference in the frequency of alleles and genotypes between the control and patient groups.