Identification of probiotic bacteria in aqueous extract from Mazafatit, Piarom and Zahedi

Agricultural Research Education and Extension Organization (AREEO), Bandar Anzali, Iran The beneficial effects of probiotic bacteria on human and animal health has been widely studied and confirmed in various cases. To obtain probiotic bacteria, Bacillus and lactic acid bacteria, dates were considered as one of the important nutrients that could be the source of isolation of these bacteria. The aim of this study was to isolate probiotic bacteria of lactic acid bacteria and Bacillus from aqueous extract of Mazafati, Pourm and Zahedi. For isolation of lactic acid bacteria and Bacillus, the extract was grown on MRS agar and agar nutrient. After 5 days of incubation at 37 ° C under aerobic and anaerobic conditions, colonies isolated with morphological characteristics and microscopic form associated including positive gram bacteria. positive and negative catalase bacteria isolated from MRS agar and nutrient agar, respectively. These bacteria were evaluated using biochemical and polymerase chain reactions. According to chemical and molecular tests, Lecunostoc mesenteroeides subsp mesenteroeides, Bacillus subtilis strain UD1022 and Pediococcus parvalus strainSC8B isolated from Mazafati, Piarom and Zahedi date extract . All three bacteria were probiotic. Discussion and According to the results of the experiments, dates are in the probiotic collection. Aqueous extract of dates can be used to enrichment and preparing of probiotic foods. Also, according to the characteristics of probiotic bacteria, aqueous extract of dates or dates may be recommended to prevent of pathogenic bacteria replacement and disease for consumers.