javad ashrafi

The chickpea (Cicer arietinum L.) is grown in more than 50 countries and is the third most important food legume in the world after beans and peas in terms of both cultivated area and total production. Chickpea is affected by a wide range of fungal and viral diseases that can cause economic losses. Among these diseases, fusarium wilts and root rot, caused by Fusarium oxysporum f.sp ciceris and F. solani, respectively Are the most important soil-brone pathogens affecting chickpea. These assays will provide plant pathologists a valuable tool to refine the control and management of diseases caused by Fusarium spesies in chickpea. The present study aimed to identify the Fusarium species associated with root rots and wilting of chickpea, as well as the pathogenic strains affecting the crop.
 

Fusarium species were isolated from roots of chickpea plants showing wilt and yellowing symptoms in different chickpea-growing regions of Kermanshah, Illam and Golstan provinces. Plant samples were cut into small pieces, washed under running water for 20 mins, sterilized with 5% sodium hypochlorite for 3 minutes, rinsed in sterile distilled water, and then placed on filter papers. Afterward, the pieces were transferred to petri dish containing potato dextrose agar media (PDA). All the plates were incubated at 25° C for 5-10 days. To determine pathogenicity, purified isolates of Fusarium species were used. Total DNA was extracted using a modified protocol previously described by Talbot et al.  Each fungal species was characterized morphologically and molecularly based on DNA sequence data for ITS-rDNA. The PCR sequencing products of the tested isolates were aligned with Clustal (ver. 2). The MEGA6 program was used for phylogenetic analysis. Aligned sequences were checked for quality and compared with deposited sequences in Gene-Bank, NCBI, using BLAST. 

Based on morphological characteristics and molecular data obtained from ITS-rDNA amplification, F. solani, F. acuminatum, F. equiseti, F. oxysporum f. sp. ciceris and Fusarium spp. were identified in the samples, among which F. oxysporum was the most dominant and common species and had the highest abundance. F. acuminatum and F. equiseti were mostly isolated from adult plants, while F. oxysporum and F. solani were isolated at all stages of growth, from seedling to podding. The phylogenetic tree, based on the comparison of ITS-rDNA sequences, classified the identified Fusarium species into four phylogenetic groups. The results showed that the use of ITS-rDNA sequencing can be used as a suitable complementary method for the identification of different Fusarium species.

Commitment to the code of professional conduct is necessary for the audit profession. Failure to comply with it will reduce the trust of the society and the credibility of the audit profession. The purpose of the current research is to investigate the relationship between social conservatism and the slippage of the auditor's professional ethics.

The research method is applied in terms of purpose and descriptive-correlational in terms of nature. The studied statistical population includes auditors working in audit institutions, members of the public accountants' society and audit organization. 320 people were selected as a sample using the random cluster sampling method from auditors working in audit institutions, members of the public accountants' society and the audit organization. The research tool is a questionnaire. SPSS software was used for data analysis.

The results show that only in the private sector, social conservatism has a significant and negative relationship with auditors' slippage in professional ethics. Also, in the private sector, gender, education, work experience and job rank variables have no significant relationship with slippage in professional ethics at the 95% confidence level. But in the public sector, among the mentioned variables, only gender and work experience have a significant relationship with slippage in professional ethics.

Based on the findings of this research, traditional and conservative values can increase independence and audit quality as a tool to strengthen ethics in this profession. In this case, the policy makers of the audit profession can encourage auditors to comply with ethics as much as possible, through training and also monitoring the implementation of ethical codes.

The purpose of this research is to identify the job preferences of the fourth generation employees, the dimensions, components and characteristics of the fourth generation human resources management specific to this generation and to prioritize the recognition of these preferences for the organization's leaders and human resource managers in the organization. It is of vital importance. In terms of results, this research is considered to be a developmental research, in terms of its purpose,it is an applied research. The indicators related to the fourth generation management of human resources in Iran's government organizations were identified and prioritized based on Delphi analysis and then based on structural-interpretive analysis. The value system factor was placed in the autonomy variable group,it was also determined that indicators such as payment system, job promotion, and communication system are included in the group of dependent variables, and leadership style indicators and feedback system are in the group. The independent variables are placed. Based on the results of this research, the payment system and the feedback system are among the most important motivational factors of the fourth generation of human resources, which managers should consider in designing and establishing a management model and an effective leadership style

The main purpose of this study is to analyze the sociological relationships between the characteristics of the fourth generation of human resources (anti-generation) in the workplace. FUZZY DEMATEL (FUZZY DEMATEL) Fuzzy Dimethole Intermediate Relationships The extracted features are measured in terms of impact and effectiveness as well as causal relationships between the components. Thirteen features are extracted for the fourth generation of human resources. Feedback and pragmatism variables have the most impact on other characteristics of the fourth generation of human resources and the two characteristics of communication with social media and pragmatism have the most impact than other characteristics. They interact with other characteristics of the fourth generation of human resources. Assessing the cause-and-effect relationships between variables revealed that the components with high expectations, technology-based, load-oriented, skill-oriented and pragmatic as five important and key causal characteristics in management and Leadership is the fourth generation of human resources.of human resources

Septoria tritici blotch (STB) caused by fungus Zymoseptoria tritici, is one of the important wheat (Triticum aestivum L.) diseases difficult to control because of the lack of wheat resistant cultivars. The use of biological control agents is one possible way for triggering host plant resistance to biotic and abiotic stresses. In this study, we examined the ability of Serendipita indica and Pseudomonas protegens CHA0-mCherry in inducing the local wheat cultivar Tajan resistance to STB. The interaction between biological control agents and the roots of wheat was evaluated. The experiment was conducted in a completely randomized design by three replicates. Spore suspension was supplied at concentrations of 107 and 109 for S. indica and bacteria isolate (CHA0-mCherry) respectively. Five treatments were applied including S. indica, CHA0-mCherry, S. indica and CHA0-mCherry co-inoculation, positive and negative control. Twentyone days after inoculation, the interaction between biological agents and plant roots were evaluated through morphological traits and qPCR. The plant resistance, disease severity, and the correlation between resistance and disease severity were assessed. Pycnidial variation and agronomic traits were also evaluated. Twenty-one days after inoculation, both biological agents clearly colonized all treated roots of all treatments except in control plants as demonstrated by qPCR analysis. Chlamydospores were observed in the S. indica-treated hosts with the CHA0-mCherry colonizing assessment showing 5×109 CFU g-1 in the root. The asexual phase of the fungal pathogen, pycnidial diameter, was reduced in S. indica treated plants more considerably than in the other treatments. There was a positive correlation between resistance and disease severity mean when calculated by Pearson’s correlation. There was a significant difference between the root length, fresh, and dry weight of root. Spore density was inversely correlated to resistance and disease severity, when compared with control, with CHA0-mCherry being the most effective in reducing the spore density. S. indica was the most effective in promoting root growth and stem biomass, when compared with control. Serendipita indica and Pseudomonas protegens CHA0-mCherry colonies showed a potential biological control activity and efficiently enhanced the plant resistance to Z. tritici in the treated wheat roots. The microbial biological control agents are very practical in crop protection against plant disease and can be very useful in sustainable agriculture. Abbreviations: PLSN: percentage of leave surface necrosis, DPI: day past inoculation, PLACL: percentage of leaf area covered by lesions, PPMLA: pycnidia per millimeter in leaf area.

Septoria leaf blotch (STB) caused by Zymoseptoria tritici, is one of the important wheat disease. The endophytic fungus Serendipita indica and the plant-protecting bacteria Pseudomonas protegens induce systemic resistance to plant pathogens. In this study, the expression rate of several defense genes in Tajan susceptible wheat cultivar treated by S. indica and P. protegens and in control plants at different times point after inoculation with Z. tritici were investigated by Real-time qPCR technique. The results showed that, the expression of PAL and LOX genes in plants treated with P. protegens was higher than other treatments. The expression of NPR1 and PR5 genes were higher in co-inoculated plants that treated by S. indica and P. protegens. The results of phenotypic evaluation of two mentioned treatments also show, the expression of these genes is effective in inducing resistance.The results indicate the active role of these two biocontrol agents in protection of susceptible Tajan cultivar against STB due to increased expression of these genes compared to control.

Yellow mistletoe is a semi-parasite plant lives in the Zagros forests on Persian oak trees. Mechanical control is one of the basic acts to manage yellow mistletoe contamination. This research aimed to evaluate the performance of mechanical methods; wood-glue, clay, black plastic, natural color and control treatment to control yellow mistletoe at different altitudes and selecting the most suitable methods was carried out in Hyanan oak forests in the northern part of Ilam province. Three research sites were selected at altitudes of 1600-1800, 1800-2000 and 2000-2200 m a. s. l. At each site, three Persian oak trees for each treatment and in total 15 trees were selected. Implementation and monitoring of treatments was done during five years (2010-2014). Results showed that the effect of control treatments on the removal rate of yellow mistletoe on the treated branches was significant. Also, the effect of altitude on the frequency of new yellow mistletoe tubers on other branches of treated trees was significant. Results showed that wood-glue and clay were 100% successful in removing yellow mistletoe. But the control performance of black Plastic and natural color was 30% and 0%, respectively. It was concluded that cutting of yellow mistletoe infected branches and coating their cutting locations with clay or wood-glue cause eliminate the yellow mistletoe and prevent its re-establishment on the limb. The combined control methods of wood-glue and clay are advisable to run for fighting against yellow mistletoe.

This study examined the effect of Crataegus hydroalcoholic extract (CHE) on intestinal ischemia-reperfusion (I/R) in rats.

Experimental study Animals- 25 adult male Wistar rats Procedures- Rats weighing 200±25 g were randomly divided into five individual groups as follows: sham group without intestinal I/R, control group with intestinal I/R, and treatment groups with intestinal I/R and 10 days oral administration of CHE at doses of 25, 50 and 100 mg/kg. Intestinal I/R was accomplished by occlusion of the cranial mesenteric artery for 30 min, followed by 60 min reperfusion. Then tissue sections of jejunum were prepared and stained with hematoxylin-eosin. Histopathological lesions including hyperemia, hemorrhage, necrohemorrhagic inflammation, and villi destruction were scored as mild, moderate and severe. Results- In histopathologic evaluation, sham and control group showed the minimum and maximum injury, respectively. The mean scores of necrohemorrhagic inflammation and villi destruction significantly decreased in 25 mg/kg CHE group compared to control. However, hyperemia and hemorrhage did not change in comparison to control (p>0.007). In the group of 50 mg/kg CHE, no pathologic lesions were observed and the results were similar to those in the sham group. The mean scores of hyperemia and necrohemorrhagic inflammation in the 100 mg/kg CHE group had no significant difference with the control group. However, the mean rank of hemorrhage and villi destruction was significantly lower than control and higher than the sham group (p<0.007).

The findings of this study indicate that CHE at the dose of 50 mg/kg has the most protective effect against intestinal I/R injury in a rat model. Therefore, Crataegus can be a promising compound against intestinal I/R injuries.

This paper is investigated social responsibility theories. Corporate social responsibility is the obligations and duties that the business enterprise are responsible for maximizing shareholders wealth to society and the environment. Research method in this paper is a library method.The results of this research show that corporate social responsibility theories are various.These theories are categorized into four groups such as instrumental theories, political theories, integrated and complementary theories and ethics theories. In instrumental theories, the corporate is seen as a tool for creating wealth and its social activities are the only means to achieve economic results. Political theories emphasize the power of corporates in society and the responsible use of this power in the political arena. Integrated and complementary theories are focused on meeting social demands by corporate. Ethical theories also emphasize the ethical responsibilities of corporates towards society. In fact, these theories focus on four dimensions of profitability, political performance, social demands, and ethical values. These findings indicate with integration these four groups of theories, a new theory of corporate social responsibility can be codified.

In this research, examineation of the disease distribution, the physiological relationship between disease agent and its sites and evaluation of cultivars resistance were done. The disease agent identified as Zymoseptoria tritici, by morphological and molecular identification based on the ITS-rDNA. Distribution map prepared by ArcGIS v.10.2 software. Five isolates selected from different regions. The isolates growth tested in different temperature and its relation to their region climatic conditions evaluated. 26 cultivars planted in the greenhouse by completely randomized design, inoculated and evaluated. As a result, the isolates growth average at different temperatures was statistically significant in P=0.01. Isolate growth average had correlation with region climatic conditions. In terms of growth, the Nokandeh isolate was the most and the Ramian isolate was lowest. The highest isolates growth was at 15°C and the lowest growth rates were at 10°C. The disease observed in 9 eparchies. Ramians and Kurdkoy identified as disease contaminated sites. Of the examined cultivars, 4 cultivars such as Falat, Mehrgan, Ghabos, and Roshan were resistant, 14 cultivars such as Shirodi, Morvarid, Moghan, Kohdasht, Gaspar, Aftab, Shahriar, Gonbad, Alamoot, Aflak, Zarin, Karaj-1, Tous, and Azar-2 were moderately resistant and 7 cultivars such as Karim, Dez, Khazar-1, Star, Hamoon, Ofogh, Eram and Tajan were evaluated as susceptible. Between isolates, the most disease severity belongs to Ramian. Considering to width distributions of STB, the existence of favorable conditions and identification of disease region center, it is possible to monitor the appearance of infection at February and March and make abroadcast of the disease prevalence. Also, by evaluating of wheat genotypes, introducing and planting the resistant cultivars, it could reduce the disease in later years.

Contagious ecthyma or orf is a poxviral disease of goat and sheep, with incidental infection appearing in humans, cows, camels, and many wild ruminants, and very rarely in dogs.
Case description: In the present paper was described gross morphology and histopathology of contagious ecthyma in a dog with a large ulcerated cutaneous mass in the neck (under the collar) which was managed by local surgery.
Clinical findings: The mass was solitary, soft, well circumscribed and grey to brown color. Cut surfaces of the mass contained discrete, round without any lobulation and liquefied foci in the centers. Histologically, there were vacuolar degeneration of keratinocytes in the stratum spinosum, marked epidermal proliferation associated with deep epidermal pegs and dermal papilla, and accumulation of scale-crust with ortho- and parakeratotic hyperkeratosis. Dermal lesions included superficial edema, marked capillary dilation, and an influx of neutrophils, followed by a marked accumulation of lymphocytes. It is guessed that the dog was fed with infected carcass.
Clinical relevance: As regards the virus has zoonotic potential, it should be given more attention.

Social responsibility provides a framework for ethical monitoring on the business enterprises activities whereby business enterprises must responsible to society and environment. This study based on financial variables and using the data envelopment analysis technique, ranks automotive companies in terms of social responsibility. Data and theoretical frameworks of the research are based on library studies and data analysis is also based on the data envelopment analysis model. Results of this research indicate that big companies in the automotive industry are less socially responsible than other companies. This suggests that big companies in the automotive industry do not have a proper social behavior toward the stakeholders, and pay less attention to social and environmental issues. Given to the outcomes of this study is suggested that regulators and policymakers in automotive industry could develop executive instructions to match social goals with businesses performance and coordinate the firm activities and responsibilities with society policies.

Type 1 diabetes (T1D), a spontaneous autoimmune disease, is associated with destruction of insulin-producing β-cells in the pancreas. Since some heat shock proteins (HSP), such as HSP70 exert a protective effect in both tissues and cells, the present study was conducted to elucidate the effects of carbenoxolone (CBX) as an HSP70 inducer on T1D. The disease was induced in male C57BL/6 mice using streptozotocin (STZ) and subjects were allocated to therapeutic 1 and therapeutic 2 groups, as well as negative and positive control groups. The treated mice (therapeutic 1 and therapeutic 2 groups) received 50 mg/kg CBX intraperitoneally every 24 hours, in the therapeutic 1 group the drug was injected before and after disease induction whereas in the therapeutic 2 group the drug was injected only after disease induction. Serum fasting blood sugar (FBS) level, cytokines production (Interferon-gamma (IFN-γ), Interleukin 10 (IL-10), and IL-17), serum HSP70 level and CD4+CD25+Foxp3+ regulatory T cell (Treg) frequency measurements were outperformed 14 days after the last STZ injection. Our results showed that in the treated groups, serum HSP70, IFN-γ, and IL-17 levels were increased in contrast to the untreated groups. The IL-10 level was markedly decreased in comparison to untreated diabetic mice (p<0.05). Moreover, it was found that the frequency of Tregs in treated mice was lower in comparison to the untreated mice but the difference was not significant (p>0.05). Our results confirm that CBX might through HSP70 induction, followed by increasing IFN-γ level leads to suppression of IL-10 production in diabetic mice resulted in toxic effects on pancreatic islet beta cells and deteriorating of disease.

In recent years, widespread tree dieback simultaneously with charcoal oak disease have occurred in Zagros forest especially in Ilam province. They dramatically caused damage to oak trees. This research was carried out to identify pathogenic fungi, to prove the pathogenicity and to investigate their pathogenic behavior during 2012-2015 in the forests of Ilam province. After several field observations across the forests of Ilam province, several samples were collected from infected parts of Persian Oak trees and transferred to the laboratory. Then, the samples were cultured to screen the pathogenic agents identified using morphological traits and validated by fungi keys. In order to prove the pathogenicity of the identified fungi, they were inoculated on two-year-old oak seedlings. In the final stage, the pathogenic behavior of fungi was studied. The results showed that fungi causing charcoal diseases in the forests of Ilam province were Biscogniauxia mediterranea and Obolarina persica. Furthermore, the fungi caused the disease on oak seedlings with dieback symptoms on the sections areas above the inoculum site. The study of the pathogenicity behavior of these fungi showed that their contamination began from inoculation site and developed in the wood, in addition to longitudinal extension, the hyphae colonized in transverse and radial directions towards the heartwood, so that it affects the xylem and phloem vessels. Considering the pathogenicity prove of these fungi and their contribution to the weakness and dieback of Persian oak trees, it would be possible to reduce the oak losses by eradicating the dead trees to prevent fungal spore spreading in the region.

This study aimed to evaluate the antifungal effect of dendrosomal nanocurcumin against Candida species and the effect of this on the expression of CaMCA1 and HSP90 genes, which may induce programmed cell death in fungal cells.
Sensitivity to curcumin and nanocurcumin in four candida species were evaluated according to CLSI (M27-S4) guideline. Then, the expression of HSP90 and CAMCA1 genes of the treated cells with dendrosomal Nanocorcumin was examined using reverse transcription polymerase chain reaction (RT-PCR) method.
Findings: The minimal inhibitory concentration (MIC) level of nanocurcumin was 1 mg/ml for Candida krusei and 0.5 mg/ml for other strains. The RT-PCR results revealed the increasing of the expression of CAMCA1 gene. This expression was significant in Candida albicans, Candida tropicalis and Candida parapsilosis while no increasing was observed in Candida krusei. The expression of HSP90 gene was decreased in Candida albicans and Candida tropicalis and was without any change in other strains.
The results of current study showed that nanocurcumin is a more efficient antifungal agent compared with curcumin. The antifungal effect is through induction of apoptosis in yeast and increasing in gene expression confirms the mechanism of apoptosis. In this study, it was observed that this effect is due to increasing in gene expression CaMCA1. Decrease and no change in gene expression of HSP90 showed that nanocorcumin does not impose antifungal effects through this gene.

Probiotic yeasts are used in production of functional foods and pharmaceutical products. They play an important role in promoting and maintaining human health. Until now, little work has been published on improving the survival of Saccharomyces in stimulated gastrointestinal condition. In this study the exposure of the yeast in the capsulate and free forms to artificial gastrointestinal conditions was assessed and the number of viable Saccharomyces cerevisiae cells during 0 to 120 mines in these conditions was evaluated by a pour plate method using sabouraud dextrose agar. Results showed the shape of the beads was generally spherical, sometimes elliptical with a mean diameter of about 50–90 µm. Also count of viable probiotic cells obtained for all the microcapsules were above the recommended levels for a probiotic food. Also decrease of approximately 4 logs was noted in the number of free cells after 2 h of incubation at pH2 and 8, when compared to decreases of about 2 logs in the all microencapsulated S. cerevisiae under similar conditions. It is concluded that microencapsulation process was significantly able to increase the survival rate of Saccha- romyces in a simulated gastrointestinal condition (p<0.05)

One of the main goals of management accounting is to calculate cost price of the products and services correctly and really and this duty has been more prominent in recent years due to rapid development of commercial activities and more competitive markets and manufacturing and commercial activities. Many evidences and results have shown that traditional cost price systems are not able to present information about cost price correctly and one cannot act in competitive markets by relying on them. In this regard, accounting knowledge designed and developed new systems such as activity-based costing system which emphasizes on correct and real allocation of indirect (overhead) expenses. Different researches have been conducted on results of this system in manufacturing agencies and almost all of them have defended this systems and its results. In this research, results of establishing this system in an academic-educational environment. Necessity of this case is one of the main financial challenges which universities face and correct management of these environments requires real calculation of cost price of the provided services. For this purpose, faculties of Biomedicalengineering, language and literature of Islamic Azad University, Tehran Research and Sciences Branch have been studied and the obtained results indicate considerable effect of applying this system in educational environments. In other words, application of system for calculation of cost price of the educational services leads to accurate and correct management and pricing of these services.

Doxorubicin (DOX) is an anthracycline antibiotic that is widely used as an anticancer agent. However, the clinical use of DOX is limited due to its toxic side effects upon non-target tissues. The aim of this study was to investigate the impact of 6 weeks of aerobic training on dox-induced toxicity in rat tissues (heart and liver). This study was conducted on thirty-two Wistar male rats randomly assigned to 1.control+salin, 2. control+DOX, 3. training+salin, 4) training+DOX groups. Groups 3 and 4 were trained on treadmill between 25 to 54 min/day and 15 to 20 m/min, 5 days/week for 6 weeks. The groups 2, 4 and groups 1, 3 received DOX (20 mg.kg-1 i.p) and saline (0.9% NaCl i.p), respectively. Animals were sacrificed 24 h after DOX and saline injections. Also, heart and liver tissue homogenization and assay parameters were performed. Doxorubicin administration cause a significant increase in malondialdehyde (MDA), nitric oxide (NO) and a significant decrease in the superoxide dismutase (SOD) activity in heart and liver tissues; however, the difference was not significant between the two tissues. Although the 6-week aerobic training+DOX caused a down-regulation of oxidant markers and up-regulation of the SOD activity in heart and liver tissues, but only changes of the SOD activity was significant between the heart and liver tissues. Our study suggests that cardiac/liver protection induced by chronically exercise in DOX treated rats is associated with inhibition of oxidative stress and increase in the antioxidant defense levels.