جستجوی مقالات مرتبط با کلیدواژه « Ovary » در نشریات گروه « کشاورزی »

Zolpidem and Passiflora incarnata can do calming and sleep-inducing effects by affecting GABA, which is one of the known brain neurotransmitters. There are evidences that GABA receptors exist in the body's endocrine glands, including the ovary.

120 pieces of Trichogaster trichopterus with an average weight of 2.76 ± 0.49 g were purchased. The fishes were divided into 8 treatments, each treatment containing 15 fishes, the control groups were: Intact and solvent, three treatment groups which received zolpidem with a dose of 5, 10 and 20 mg/kg and three treatment groups receiving Passiflora incarnata extract with doses of 25, 50, and 100 mg/kg . Intramuscular injection was done every other day to reduce stress on the fish for twenty days. Then the fishes were dissected and the ovarian tissue was separated for examination with light and electron microscope and steroid hormones were measured.

The results showed that 17-beta-estradiol hormone increased in all doses of zolpidem and decreased in all doses of Passiflora incarnata extract compared to the control groups (p≤0.05).In all three doses of zolpidem, the oocytes were mostly in the vitellogenic phase compared to the control group.

while in the treated fish in all three doses of Passiflora incarnata, they were mostly in the prenuclear phase.

The aim of this study was to optimize different concentrations of some growth regulators and introduce the effective explants which lead to increase callus induction and regeneration efficiency in Catharanthus roseus L. and recognize growth stage which produce the highest amount of vincristine. The experiment was conducted as a factorial with a completely randomized design. The first factor was explant type at eight levels and the second factor was different concentrations of growth regulators at nine levels. The results showed that the highest percentage of callus induction was observed the medium containing 8 μM 2,4-D with 2 μM BAP in single node (100 percent), hypocotyl (100 percent), leaf (100 percent), root (99.16 percent), ovary (98.33 percent), petiole (95.83 percent), anther (94.16 percent) and cotyledon (93.33 percent) explants, respectively. Calluses derived from hypocotyl, anther and ovary explants were able to produce highly efficient somatic embryos. 0.8 g L-1 casein hydrolyzate cause the highest percentage of embryogenesis. The maximum germination percentage was obtained from explants drived from hypocotyl (53.66 %), anther (51.00 %) and ovary (46.00 %). Also, the regeneration percentage of hypocotyl, anther and ovary explants was 78, 73 and 68 percent, respectively. The topmost amount of vincristine in comparison with different types of calluses and different stages of growth was obtained from plants regenerated from callus which derived from anther, ovary and hypocotyl explants and the amount was 1.4 μg g-1 dry weight more than non-embryonic callus of the mentioned explants.

Estimates show that in the world about 30 million goats and in Iran about five million Cashmere goat are bred. One of the most important of these breeds is the Raini Cashmere goat. Steroid hormones perform their physiological functions by regulating the transcription of target genes. Receptors for these hormones play a role in osteoporosis, inflammation and cancer through transcription networks. Estrogen receptor (ER) is a transcription factor that mediates the function of estrogen in many physiological and pathological processes. There are two estrogen receptors, ESR1 and ESR2, which are located in the reproductive organs. Reproduction rate, especially fertility, is one of the most important economic traits in animal production and is regulated by genetic and environmental factors. Therefore, the aim of this study was to investigate the expression profile of ESR2 gene in different tissues of fluffy goats using Real Time PCR.

Sampling of testis, ovarian, uterine, breast, brain, kidney and heart tissues was performed from a male and a female goat (3 replicates from each tissue). Total RNA was extracted from the tissues and the quality and quantity of the extracted RNA were evaluated. The cDNA was then synthesized and Real Time PCR was performed. The GAPDH housekeeping gene was used as a control. Melting curves were examined and data obtained from Real Time PCR were analyzed. 

The results of Real Time PCR curves and observation of electrophoresis of PCR products on 2% agarose gel showed that ESR2 gene was expressed in testis, ovary, uterine, breast, brain, kidney, and heart tissues. The highest level of expression was observed in the ovary and then the brain and uterus and the lowest level of expression was related to kidney, heart and testis tissues.

According to the results of the present research and the results of other studies, it can be concluded that ESR2 plays an important role in female fertility, because in the present study it was found that ESR2 is much more expressed in ovarian tissue than other tissues. Therefore, ESR2 is likely to be essential for female fertility, and the results of this study provide the basis for future research to describe the role of the ESR2 gene as a candidate gene for better fertility and normal physiology in domestic animals, especially goats.

This study was conducted to determine the effect of different levels of omega-3 and omega-6 calcium fat powders on performance, hatchability, serum lipids, abdominal fat, and ovarian follicle count in Ross-308 broiler breeder hens (65 week-old). The experiment was performed in a repeated measurement design with 84 hens and 28 roosters with seven treatments (four replicates) during three months. The experimental groups included control group (no fat powder), 1.5 and 3% of animal omega-3 calcium fat powder of fish oil origin (ω3F), plant omega-3 calcium fat powder of linseed oil origin (ω3L), and omega-6 calcium fat powder of soybean oil origin (ω6S). The results showed that the highest average of HDEP was belonged to the experimental groups containing 1.5% ω3F (61.98), and ω3L (60.25) without significant difference with together (P> 0.05). However, among experimental groups, the highest egg mass (44.78 g/h/d) was obtained from consumption of 1.5% ω3F (P<0.05). The highest concentrations of triglycerides (1806.8 mg/dL), cholesterol (206.25 mg/dL), and low density lipoprotein (244/25 mg/dL) of serum were belonged to control group that was significantly different from other experimental groups (P<0.05). The percentage of hatchability of fertile eggs was improved by consuming diets containing fat powder, except 3% ω6S, when compared to control (P<0.05). Overall, considering the production performance, hatchability and economic explanation of diets, it seems that addition of omega-3 calcium fat powder at the level of 1.5% (ω3F and ω3L) is affordable and can have a positive effect on old broiler breeder hens.

In this study, gonad structure and oogenesis of ghost crab were studied based on macroscopic and microscopic analyses from January 2016 to February 2017. Female crabs were collected from sandy beach of Salahk port, Qeshm Island, monthly. In the laboratory, the crabs’ ovaries were dissected and prepared for histology. Based on external and morphological features, gonad developmental stages were investigated and afterwards each stage was microscopically described. According to the results, six ovarian developmental stages were recognized including: stage I: immature, stage II: rudimentary, stage III: developing, stage IV: intermediate, stage V: advanced, stage VI: spent. During oogenesis, the number of oocytes in secondary growth increased in the ovaries which was associated with a change in coloration and increase in the gonad volume. Oocytes growth process was classified in six stages: early primary growth, late primary growth, early secondary growth, mid-secondary growth, late secondary growth, and full-growth. According to results, shaping of perinuclear yolk complex observed just in late primary growth stage. Furthermore, starting of second growth was simultaneous to appearing of yolk globules in ooplasm. Also, most of oocytes were visible as light pink in full-Grown stage.

Favorable pollen transition on the stigma and pollen tube penetration is the most important for fruit setting in the apple. Boron is the main factor for pollen tube growth. A probable responsibility for boron in pollen tube growth may include vesicle making, transportation, fusion or the successive formation of the pollen cell wall. Pollen tube cell walls are rich in polypeptides, glycoprotein, polysaccharides and arabino furanosyl which are known to form physically potent complexes with boron. Maximum cell boron content is located in the cell wall where it is associated with pectin compounds. However, under definite field conditions, foliar applications of boron have been resulted to raise fruit set by as much as 100% in many researches.

In this study effects of acid boric by 0 (as a control), 1000 and 2000 ppm concentrations was studied on the pollen tube penetration percentage to style and ovary in six crosses among four apple cultivars including "Fuji", "Gala", "Goldn delicious" and "Red delicious' by florescence microscopy method, 72 and 120 hours after pollination. All of the trees were 12 year old on EM126 rootstocks and foliar sprayed by H3BO3 as the boron source (0, 1000 and 2000 mg.L) in the October. Crosses among the cultivars were programmed as ♀Red delicious × Golden delicious ♂, ♂ ♀Gala× Fuji, ♀Red delicious × Fuji♂, ♀Red delicious × Gala ♂, ♂ ♀Golden delicious × Fuji and ♀Golden delicious × Gala ♂. For each cross four repeats in all direction of the tree were regarded and in each repeat at least two branches with 60 – 100 were labeled in winter. Selected female cultivar's flower buds at ‘D’ stage were bagged to prevent the entrance of foreign pollens on the closed pistils. Pollens collected from the male cultivar flower buds and maintained in freezer until using in the field pollination time. Pollen germination was tested in an in vitro medium before field application on the pistils. Selected female cultivar's flowers were pollinated with selected male parent pollen when the pistils were acceptable for pollens and repeated after 24 hours. Determination of pollen germination percentage and tube growth on the pistil and different parts of the styles was studied by the florescence microscopy method 72 and 120 hours after pollination healthy pistils were separated from the branches and fixed in FAA solution and prepared for fluorescence microscopy observation as indicated in Ortega and Dicenta (2006). In each pistil the number of germinated pollen grains on the stigma, the number of pollen tubes in the first, second and third section and so, in the ovary were determined by a fluorescent microscope. Because of the five partitions of the apple flower stigma and style; the mean of the five parts was evaluated for each of them. The experiment was carried out as a factorial based on completely randomized design with three factors including B in three levels (0, 1000 and 2000 mg.L), time (0, 72 and 120 hr after pollination) andsix crosses in five replications (at least 10 style per cross). Data was analyzed following SAS (26) software. Mean values were analyzed by Duncan’s multiple range test.

Results showed that all of the boron concentrations, time after pollination and crosses were significantly affected pollen germination percentage on the stigma and tube penetration to different parts of the style and ovary at P<.01 level. Maximum pollen germination percentage on the stigma (33%) was observed in ♀Goldn delicious × Gala ♂ cross which treated by 1000 ppm boron 120 hr after pollination. Also, maximum pollen tube penetration to ovary was observed in ♀Red delicious × Golden delicious ♂ cross (32.06%). However, best compatibility between them and boron effects on the pollen germination percentage and tube growth in the style. It was revealed that in all of the crosses pollen germination percentage and tube growth in the style was increased by boron treatments.

However, boron is one of the main growth elements by its role in the cytokinin biosynthesis which is the most important hormone affects pollen tube growth and penetration. In this research the foliar application of boron increased pollen germination and pollen tube growth in all of the studied four apple cultivars crosses. However, increasing the boron concentration led to increased pollen germination and pollen tube growth linearly.

This research was conducted to investigate the production process of goldfish and sexual maturity of oocytes at the time handling of breed. In the mosquito breeding complex, Dr. Yusefpour was take. For this purpose, 250 nambber female mature with middhe weiths 23.74g . Monthly, 50 female breed from tank selected randomly and after weighingand measure the length, gonad and liver weith, liver and gonadotropic index were determined. Each month, three ovarian tissue samples were prepared and, after the dehydration and clarification stages, the coloring was crried out on samples. Of each sample ovary was prepared the three areas, front, center and behind. The resulths showed that in the early months of sexual (December), a percentage of oocytes stage One and Two in compared to the oocytes of Three and Four than (April). In all months in each Three area front, center and behind oocytes were seen in stages three and four. The frequency of oocytes in stagevfour increased with increasing of sexually stage (April), increased in the ovary front.

Apple is one the most important horticultural crops in Iran. As the most apple cultivars are self-incompatible, having a good fruit set requires favorable cross-pollination. However, this phenomenon is related to a series ofcomplex processeswhich happens on thestigma. Zinc is one of the main factors that affects pollination and has an important role in increasing the percentage of pollen germination thereby increasing fruit set. It has been reported that zinc is referred to be as a main growth element because of its role in hormone production in buds specially auxin biosynthesis. Auxin is the most important hormone which affects pollen tube penetration to the style and so, fruit set in plants. Zinc deficiency in the apple orchards primarily affects the above ground portions of trees, resulting in poor leaf and shoot growth, reduced flowering and fruit set, and reduced size and coloring of fruit. Zinc is also important in the movement of calcium within the tree. Using zinc sprays is the most consistent and cost effective way to apply this element in apple tree orchards.
In this research, the effect of zinc solution in three levels (0, 3000 and 5000 mg.l) was studied on pollen penetration to the ovary in the crosses among four apple cultivars including Golden Delicious, Red Delicious, Gala, Fuji. Pollen penetration was studied 72 and 120 hours using fluorescent microscopy technique in the laboratory after field pollination. Crosses among the cultivars included 1- ♀Red delicious × Golden delicious♂, 2- ♀Galla × Fuji♂, 3- ♀Red delicious × Fuji♂, 4- ♀Golden delicious × Fuji♂ and ♀Golden delicious × Galla♂.
Results showed that the effects of zinc, crosses and their interaction were significant on pollen germination and tube penetration in the primary, middle of the stigma and top of the ovaries. The highest percentage (59.4%) of germination on the stigma was observed in the cross (♀Golden Delicious × Gala ♂), in 120 hours and the highest penetration of pollen tube in ovary (18.08%) was observed at the cross point of 5000 ppm of Zinc, in 120 hours respectively.
In this research the foliar application of Zinc (until 3000 mg.l) increased pollen germination and tube growth on the stigma in all of the studied four apple cultivars. Although, increasing zinc concentration to 5000 mg.l showed negative effects on the both characteristics which showed the tolerance range of the cultivars to the zinc concentration based on their genetic traits. However, increasing the Zinc concentration to 5000 mg.l led to increased pollen tube growth and penetration to the different parts of the styles in all of the crosses linearly.

This experiment was conducted to evaluate the effect of different lipid sources and organic zinc supplementation on performance, egg bacterial activity and ovary morphology of laying hens for 10 weeks. with 288 sixty weeks old birds by employing a completely randomized design with factorial arrangement of 3×4 with 12 treatments, 4 replicates and 6 hens each. The factors of interest were 4% lipid from different sources (soybean oil, fish oil and tallow) and various levels of zinc (40, 100, 200 and 300 mg/kg of diet). In all three lipid sources, significant reduction (P

An experiment was conducted to determine the effects of Purslane extract (PE) on performance, egg quality and ovary quantitative characteristics of laying hens. One hundred and twenty laying hens (Leghorn, Hy-Line W36) were used in a completely randomized design with 4 treatments, 5 replicates and 6 hens per replicate. Four experimental treatments were, control (Corn-Soy basal diet) and 0.1, 0.2 and 0.3 % of Purslane extract which added to basal diet. The results indicated that there were no significant differences on performance parameters of laying hens (P>0.05). Haugh unit traits, shell thickness and egg yolk percentage did not affected by different levels of PE (P>0.05). However, shell strength, shell percentage and yolk color, increased with inclusion of PE in diet rather than control group. Shell strength in 0.3 % of PE had highest value (2.02 kg/cm2). Primary antibody response did not affected by experimental diets (P>0.05), but, secondary antibody significantly (P

Mature ovary culture was used to develop inter-specific hybrid between Populus caspica L. and P. nigra. Manual pollination of Populus caspica، female flower was conducted by P. nigra L. pollen grains، using twigs and pod breeding system. Developed ovaries in different developmental stages (10، 14 and 21 days after pollination) were then aseptically transferred to hormone growth regulator free MS culture media supplemented by two different sucrose concentrations (30 and 60 g/L) for embryo germination. Analysis of collected data indicated that there were highly significant differences between embryos age for germination at α=0. 01 level. Fourteen days old embryos showed highest embryo germination (85%) then the other two There were no differences between culture media for embryo germination but highly significant differences was observed between culture media for growth rate of plantlets at α=0. 01 level. MS medium supplemented with 60 g/L sucrose showed 4. 41 cm growth rate while average 2. 09 cm was observed for MS medium containing 30 g/L. Plantlets were transferred to the same medium within jars، before being transferred to the potting soil. One hundred seventy two plantlets were successfully acclimatized in green-house and transplanted to the field.

In the present study، Structure of the adult ovary and oogenetic mode were examined in the freshwater crab Sodhiana iranica. Female crabs were collected From Eelood spring located in Hormozgan province، southern Iran. In the laboratory، ovary of crabs were removed and prepared for Histological examination. An H-shaped ovary consisting of a pair of long ovarian sacs connected by a narrow bridge tube is located in the cephalothorax on the dorsal side of the stomach. A short oviduct with a seminal receptacle is connected with the posterior end of each ovarian sac، and a genital pore opens on the sternum of the sixth thoracic segment. The ovarian wall consists of a layer of ovarian epithelium that infolds to form a number of oogenetic pouches of various sizes. Each oogenetic pouch contains an egg or a relative large oocyte in its lumen. Germaria containing oogonia، very early previtellogenic oocytes، and somatic interstitial cells are located in the ovarian epithelium near the necks of the oogenetic pouches in the anterior regions of the ovarian sacs and are randomly scattered throughout the ovarian epithelium in the posterior regions of the ovarian sacs. In cross section، the germaria appear to be concentrated into a central germarial cluster in the ovarian sac. Mature eggs are ovulated from the oogenetic pouches into the ovarian lumen، transferred from the ovarian lumen into the oviducts، fertilized there by sperm stored in the seminal receptacles، and then oviposited through the genital pores. The female reproductive system is surrounded wholly and tightly by a thin، cellular، membranous sheath، which has often been mistaken as the ovarian epithelium in some decapod crustaceans.