mohammad ali nilforoushzadeh

 Vitiligo poses a significant challenge, particularly in treatment-resistant areas such as acral regions. This study aimed to evaluate the effectiveness and safety of combining fractional CO2 laser and microneedling with conventional therapies for non-segmental vitiligo in resistant areas.

 A randomized clinical trial with a paired design was conducted on 84 individuals with non-segmental vitiligo and treatment-resistant lesions. Participants in two separate groups received various combinations of interventions, including narrowband ultraviolet B (NB-UVB) and topical steroid (Clobetasol ointment) as conventional treatments, with fractional CO2 laser or microneedling as add-ons for four symmetrical treatment-resistant lesions. The treatment duration was 16 weeks, followed by observation until six months post-intervention for each patient, and it focused on assessing the percentage of repigmentation, safety, patient satisfaction, and potential adverse effects.

 The results of the study revealed a significant difference in average efficacy, with treatments involving fractional CO2 laser and microneedling compared with other interventions without them showing a higher percentage of repigmentation. No notable distinction in repigmentation patterns was observed, except for slightly higher marginal repigmentation with microneedling. Side effects, including pain, burning sensation, and erythema, were more frequent, with no significant difference between the groups. Itching and crusting had a substantial difference in the two groups. Patient satisfaction levels were comparable, with the first group slightly more than the second.

 In conclusion, combining NB-UVB and topical steroid therapies with the fractional CO2 laser or microneedling seems to be effective for vitiligo treatment in resistant areas. Attention to variations in patients’ characteristics and specific body locations is necessary when applying these modalities. This study provides valuable insights into novel approaches to managing vitiligo and highlights the potential benefits of these innovative treatments in combination with conventional therapy.

Vitiligo is an autoimmune disease of the skin that affects both sexes and people of any age. The genetic and environmental factors are involved in the vitiligo etiology. Helicobacter pylori (H. pylori) has an important role in vitiligo progression. Therefore, the present study evaluated H. pylori seropositivity in vitiligo patients compared to healthy individuals. H. pylori infection was investigated in 210 vitiligo patients and 127 sex- and age-matched healthy controls using Enzyme- Linked Immunosorbent Assay (ELISA) test. The data was analyzed using SPSS software version 20.0, and the groups were compared using T-test and ANOVA tests. P < 0.05 was considered statistically significant. Vitiligo patients had higher median levels of IgG (29.68 ± 28.28 RU/mL) than (19.08 ± 20.12 RU/mL) in healthy controls (P < 0.000). Moreover, there was no significant difference between groups based on the level of IgM (P < 0.207). In the vitiligo group, IgG or IgM means were different compared to age (P < 0.33)/ (P < 0.017) and early symptoms (P < 0.00) (P < 0.02), respectively. Unlike IgG, there was a significant difference between the mean level of IgM, the onset age of vitiligo (P < 0.022), and the duration of the disease (P < 0.05). Moreover, males and females with vitiligo had a higher seropositivity to H. pylori antibodies than the control group. Vitiligo was found to be significantly associated with H. pylori in Iranian patients. Therefore, it seemed probable that H. pylori had an important role in the initiation or progression of disease activity in vitiligo.

Background and Objectives The use of stem cells for skin wound healing is still facing serious challenges.This study aims to determine the effectiveness of human adipose tissue-derived stem cells (ADSCs) sprayin histologically improving skin wounds in male diabetic rats.Subjects and Methods In this experimental study, mesenchymal stem cells were isolated from adiposetissue and cultured and characterized by evaluating the expression of specific CD markers. Microbialcontamination in samples was assessed before treatment. Then, 18 male Wistar rats were dividedinto control and treatment groups. Diabetes was induced by a single injection of streptozotocin. Then,wounds with a diameter of 0.8 cm were created in the back of the rats. The ADSCs were sprayed on thewound area in the treatment group. Wound healing was assessed on days 7, 14, and 21 after treatmentusing visual observation and histopathological examination. The data were analyzed using ANOVA.Results Wound closure was accelerated on days 7, 14 and 21 in the treatment group compared to thecontrol group. Histological examination showed that the skin thickness, angiogenesis, and epithelializationwere higher in the treatment group on days 7, 14, and 21 compared to the control group.Conclusion The spray of ADSCs on the wound area can accelerate the healing process and can be usedfor skin wound repair.

Nano-scaffolds loaded with bioactive compounds such as ZnO nanoparticles (ZnO-NPs), as tissue-engineered artificial skin grafts, can be a suitable substitute for extracellular matrix and greatly contribute to accelerating chronic wound treatment by decreasing the chance of bacterial infection.

Silk fibroin nanofiber was fabricated by using electrospinning and three-dimensional porous hybrids (3DPH) nano-scaffold with composite of sodium alginate/ZnO-NPs solution and silk fibroin electrospun nanofibers by adopting freeze-drying method. Successful configuration of nanofibers and porous nano-scaffolds were confirmed using field emission scanning electron microscopy (FE-SEM). Antimicrobial activity, cell attachment, and cytotoxicity evaluation of scaffolds were performed by employing disk diffusion method, L929 cell culture, and MTT assay, respectively.

Antibacterial analysis of 3DPH nano-scaffolds revealed their appropriate antibacterial activity against the Staphylococcus aureus and the Escherichia coli bacteria. Furthermore, the results from cytotoxicity and cell adhesion analyses indicated the appropriate cell attachment, viability, and proliferation on the silk fibroin nanofibers and 3DPH nano-scaffold, which are fundamental for wound healing and skin dermo-epidermal grafts.

In sum, silk fibroin nanofiber as an epidermal graft and 3DPH nano-scaffold loaded with ZnO-NPs as a dermal graft were fabricated. Moreover, 1.5% (w/v) concentrations of ZnO-NPs were selected and incorporated into the 3DPH nano-scaffold. Considering the promising results of biological analyses, the nanofibrous and 3DPH nano-scaffolds composite may have been suitable for skin dermo-epidermal grafts and skin regeneration.

There are various types of treatment targeting healing traumatic or accidental skin scars. Transplantation of skin grafts and surgical alternatives, including autologous transplantation of melanocyte-keratinocyte suspension, have also been suggested previously. This study is representing a case of previous skin graft transplantation, complaining of scar formation and discoloration on the transplanted segment.

The patient was a 37-year-old lady. This patient underwent melanocyte-keratinocyte suspension transplantation and narrow-band ultraviolet B (NUVB) therapy and could reach 40% re-pigmentation enhancement. This method could be introduced as an efficient and safe method of approaching facial scarring.

This method could be introduced as an efficient and safe method of approaching facial scarring.

Dark circles and wrinkles under the eyes are common cosmetic problems, caused by various conditions, especially aging and overproduction of melanin in the epidermis or dermis of the skin. In addition to the application of topical lightening agents, different types of lasers, especially the Q-Switched ND:YAG laser, has been used for the treatment of cutaneous hyperpigmentation. Because of a high prevalence of idiopathic eye dark circles (EDCs) or periorbital melanosis and a poor response to available therapies, we decided to evaluate the efficacy and safety of the Fractional QS 1064 nm ND:YAG Laser through a before-after trial.

18-65-year-old patients with skin Fitzpatrick phototype of I-V and without any usage of a topical or systemic therapeutic regimen (2-4 weeks before the trial) were enrolled in the study. Each patient was treated with 6 sessions of the Fractional QS 1064 nm ND:YAG Laser at 2-week intervals and assessed for response and possible side effects or recurrences through 4 outcome measures, including Visoface-based color and erythema, melanin index and lightness (Before the fourth and sixth sessions of the therapy; also 1 week and 3 months after finishing the trial).

The changes in Visoface-based color and erythema, the melanin pigment amount by the Mexameter (melanin index) and the degree of lightness by the Colorimeter of patients after 6 months of intervention were statistically significant (P<0.001).

The fractional QS 1,064 nm ND: YAG Laser is an effective and safe therapy in EDCs since objective outcomes like the reduction of the melanin index and improving lightness and subjective ones like the reduction of darkness and erythema were confirmed.

Diabetes is rising worldwide and impaired wound healing is one of its major complications. This study aimed to determine the effects of adipose-derived mesenchymal stem cells (MSCs) on wound healing in diabetic rats.

In this experimental study, abdominal adipose tissue was obtained from 10 patients who underwent an abdominoplasty. MSCs were isolated from adipose tissue and characterized using flow cytometry. Streptozotocin was used to induce diabetes in 10 male rats. Full-thickness excision wounds were punched on the back of each rat by a punch biopsy (0.8 cm in diameter). Animals were divided into two groups: the control group (receiving normal saline) and the group treated with MSCs. After treatment, wound healing was evaluated by photographic methods on days 7, 14, and 21.  

The isolated cells from adipose tissue expressed specific positive markers of MSCs. Importantly,the wound area significantly decreased in MSCs treated group on days 7 (P<0.05) and 14 (P<0.01) compared with days 0 and 7, respectively. The wound area decreased significantly in control animals on day 7 compared to day 0 (P<0.01), however, it did not significantly change on day 14 compared with day 7. On day 21, wounds closed in control and treatment groups.

Spraying the human adipose tissue-derived MSCs causes faster skin wound healing and therefore, this method can be considered for diabetic wound healing.

Cell therapy is one of the most challenging methods in the healing of burn wounds in the world. The present study aimed to determine the effect of collagen hydrogel with adipose-derived mesenchymal stem cells on burn wound healing in an animal model.

The present study was experimentally performed on 24 Wistar rats. Burn wounds were created on the skin of rats. Then, the rats were divided into a control group treated with normal saline and a group treated with collagen hydrogel with adipose tissue-derived mesenchymal stem cells. The healing process of burn wounds was quantitatively and qualitatively evaluated at intervals of every seven days for 21 days. The obtained data were analyzed in the form of burn wound healing parameters using one-way ANOVA.

The wound area was traced on days 7, 14, and 21 in the treatment group which was significantly smaller than that in the control group, indicating a faster wound healing in the treatment group compared to the control one  (P>0/05).

Mesenchymal stem cells transferred by hydrogel to the wound site rapidly heal burn wounds; therefore, evaluating the use of this dressing in the healing of burn wounds is important in clinical cases.

Cell therapy is one of the most challenging methods in the world in repairing burn wounds. The aim of this study was to determine the effect of chitosan hydrogel containing adipose-derived mesenchymal stem cells on burn wound healing in an animal model.

This study was performed experimentally using 24 Wistar rats. Burn wounds were created on the skin of rats and the animals were divided into two groups (control treated with normal saline) and the group treated with Chitosan hydrogel containing adipose tissue-derived mesenchymal stem cells. The healing process of burn wounds was evaluated quantitatively and qualitatively after treatment on days 7, 14 and 21 compared to the day 0. The data were analyzed using one-way analysis of variance.

Concentration of 10% chitosan had no significant cytotoxic effect on mesenchymal stem cells and had proper consistency and clarity and SEM microscopy showed suitable porosity in chitosan. The expression of specific markers in mesenchymal stem cells and their potential for differentiation into adipocytes and bone cells confirmed the mesenchymal nature of the cells. Wound area on days 7, 14 and 21 in the chitosan-treated group containing mesenchymal cells was significantly smaller than the control group.

Mesenchymal stem cells transferred by chitosan to the wound site cause accelerated healing of burn wound and therefore, it is important to evaluate the use of this dressing in the healing of burn wounds in patients.

Hair loss is a prevalent medical problem in both men and women. Maintaining the hair inductivity potential of human dermal papilla cells (hDPCs) during cell culture is the main issue in hair follicle morphogenesis and regeneration. The present study was conducted to compare the effects of different concentrations of exosomes derived from human adipose stem cells (hASCs) and platelet-rich plasma (PRP) on the proliferation, migration and expression of alkaline pholphatase (ALP), versican, and smooth muscle alpha-actin (α-SMA) in human DPCs.

In this experimental study, hDPCs, human hair DPCs and outer root sheet cells (ORSCs) were separated from healthy hair samples. The protocol of exosome isolation from PRP and hASCs comprises serial low speed centrifugation and ultracentrifugation. The effects of different concentrations of exosomes (25, 50, 100 μg/ ml) derived from hASCs and PRP on proliferation (MTS assay), migration (scratch test) and expression of ALP, versican and α-SMA (real time-polymerase chain reaction) in human DPCs were evaluated.

The flow cytometry analysis of specific cytoplasmic markers showed expression of versican (77%) and α-SMA (60.8%) in DPCs and K15 (73.2%) in ORSCs. According to NanoSight Dynamic Light Scattering, we found the majority of ASCs and PRP-exosomes (ASC-Exo and PRP-Exo) to be 30-150 nm in size. For 100 μg/ml of ASCs-Exo, the expressions of ALP, versican and α-SMA proteins increased by a factor of 1.2, 2 and 3, respectively, compared to the control group. The findings of our experiments illustrated that 100 μg/ml of ASCs-Exo compared to the same concentration of PRP-Exo significantly promote DPC proliferation and migration in culture.

This study introduced the potential positive effect of ASC-Exo in increasing the proliferation and survival of DPCs, while maintaining their hair inductivity. Thus, ASCs-Exo possibly provide a new effective procedure for treatment of hair loss.

The number of patients suffering from diabetic ulcers has been increased in recent years and the current therapies have faced failure. This study aimed to investigate the effects of Wharton’s jelly stem cells (WJMSCs) on the diabetic wound in an animal mode.

During this laboratory experimental study carried out in Skin and Stem Cells Research Center from March 2021 to November 2021, WJMSCs were isolated and their differentiation capability to osteocytes and adipose cells was assessed using the colorimetric method, and the expression of specific markers was evaluated using flow cytometry. 12 male Wistar rats weighing 200 to 250 grams were purchased from the Pasteur Institute and kept in the animal room in standard condition. Streptozotocin was used to induce diabetes in male Wistar rats. Animals were divided to control (normal saline injection: n=6) and WJMSCs injection (n=6) groups. Wounds with 0.8 cm in diameter were made on the back of rats. After subdermal injection of normal saline and WJMSCs, wound healing was evaluated 7, 14 and 21 days using the photography method. Data were analyzed using a t-test and analysis of variance.

The results showed that the isolation process should be performed no later than a few hours after the cesarean section. Storing the sample for one day or more caused sample contamination leading to significant failure in cell proliferation and differentiation. WJMSCs were positive for specific mesenchymal stem cell markers (CD44, D73, CD90 and CD 105, and negative for CD45 and CD 34. They were capabale to differentiate into osteocytes and adipose cells and had a high viability rate (83.1%). Subdermal injection of WJMSCs in diabetic rats resulted in acceleration of diabetic wound healing compared with the control group.

Subdermal injection of WJMSCs can effectively accelerate diabetic wound healing. According to which, applying Wharton’s jelly stem cells can be considered in cell therapy particularly in the field of diabetic wound healing.

The efficiency of stem cell isolation, culture, and biological characterization techniques for treatment is facing serious challenges. The purpose of this study was to provide a protocol for isolation and culture of three types of mesenchymal stem cells (MSCs) derived from the human placenta, amniotic membrane, and umbilical cord with high efficiency used for cell therapy.

During this experimental laboratory study, 10 complete placenta samples were prepared from cesarean section mothers. The protocol for isolation and culture of mesenchymal cells from the placenta tissue, umbilical cord, and amniotic membrane was enzymatically optimized. The morphological features of mesenchymal cells were investigated using an inverted microscope and their biological features were measured using flow cytometry. The differentiation potential of the cells was evaluated by measuring their differentiation capacity into osteocytes and adipocytes. The absorption and reflectance features of the cells were recorded by optical spectroscopy. Finally, the data were statistically analyzed.

The expression of CD44, CD73, CD90 and CD29 markers in human placenta tissue-derived cells was significant. CD14, CD34 and CD45 markers were not expressed or were slightly expressed. These cells were highly viable and successfully differentiated into osteocytes and adipocytes. MSCs absorbed more light than visible light by showing light absorption peaks at wavelengths of about 435 and 550 nm.

The protocol used in this study for isolation and culture of human placenta tissue-derived MSCs had significant efficiency for the production of MSCs for use in cell therapy and tissue engineering

Wound healing can have a very important impact on the patients’ quality of life. For its treatment, wound dressings have vital and effective uses. Indeed, the use of a proper wound dressing can improve the healing process and duration. Recently, wound dressings with unique properties have been prepared using natural hydrogels. In addition to the general wound characteristics, new generations of wound dressings, such as those lasting longer on the wound, can have specific properties such as transferring allogeneic cells to enhance the healing effect and speed up the healing process. The present study aimed to prepare a gelatin-based hydrogel and to characterize it for therapeutic purposes.

In this experimental-laboratory study, a gelatin hydrogel was made using a microbial transglutaminase (mTG) enzyme. The prepared hydrogel was evaluated in terms of appearance, physical, and chemical properties. To investigate the biological properties of the hydrogel, cells were cultured on it and the toxicity of the hydrogel for the cells was investigated. The location of the cells on the hydrogel was imaged via an electron microscope. The absorption and reflectance characteristics of the hydrogel were recorded by optical spectroscopy. Data were collected and statistical analysis was performed.

The results showed that the mTG gelatin hydrogel had a uniform pore size and good physical, chemical, and mechanical properties for use in wound healing. Cell experiments showed evident cell proliferation and high viability. The results also revealed that the cells grew vigorously and adhered tightly to the hydrogel.

The preparation of a gelatin hydrogel under GMP conditions can be considered in the healing of diabetic wounds and burns.

Acne vulgaris is a disease of pilosebaceous unit with multifactorial pathogenesis and threats patients’ social functioning. There is a growing research to find faster, more effective, and easy to use treatments. The aim of this study is to evaluate the efficacy of benzoyl peroxide 5% (BP) with and without concomitant intense‑pulsed light (IPL) therapy in mild‑to‑moderate acne vulgaris.

In this controlled trial, 58 eligible patients with mild‑to‑moderate acne and Fitzpatrick skin phototype III and IV were randomly allocated to two groups. All patients were asked to use a thin layer of BP every night. The IPL therapy was administered at the end of first, 2nd, and 3rd months. Acne Global Severity Scale (AGSS), Acne Severity Index (ASI), and total lesion counting (TLC) along with patient satisfaction were recorded. Patients were also examined 1 month after the final therapeutic visit.

The IPL group showed greater reduction in AGSS (P < 0.001) and TLC (P = 0.005) than the control group. However, the difference in ASI was not significant (P = 0.12). Patients in IPL groups were more satisfied than control group (P < 0.001).

Adding IPL to BP can result better response to BP alone. In acne treatment, combination therapy such as IPL and other topical agents should be kept in mind.

Psoriasis is a chronic skin condition that emotions can affect symptoms and related problems. But are cognitive-behavioral-emotional therapy and mindfulness-based cognitive therapy effective on symptoms and related issues? And which treatment is most effective?

The aim of this study was to compare the effectiveness of cognitive-behavioral-emotional therapy and cognitive-based cognitive therapy on socio-physical anxiety, itching, and severity of psoriasis in psoriasis patients.

This study was a quasi-experimental study with pre-test, post-test and two-month follow-up with control group. The population consisted of 30 patients referred to dermatology research center of Tehran University of Medical Sciences who were selected by convenience sampling and randomly assigned to control group (5 male and 5 female) and first experimental groups (5 Men and 5 women) and second (5 men and 5 women) were recruited. Tools include Emoji-Cognitive-Behavioral Cognitive-Behavioral Therapies (SDWC) (2006) and Datilio Model (2010) and Kabat's Mind-Awareness Cognitive Therapies. Zain (1990) Hart et al. (1989) Social-Physical Anxiety Scale, Visual Analogue Scale, and Severity and Level Index of Psoriasis Curtis (2009). Data were analyzed using repeated measures ANOVA.

Both treatments reduced socio-physical anxiety with greater efficacy of cognitive-based cognitive therapy. Both interventions were equally effective in reducing itching. Emotional-cognitive-behavioral therapy, but not mindfulness-based cognitive therapy, reduced the severity of psoriasis. At the two-month follow-up, changes remained stable over time (P<0/001).

These two treatments are useful interventions to reduce socio-physical anxiety and symptoms and severity of disease in psoriasis patients.

This case series describes successful management grade 3 burn ulcers in 10 diabetic patients using Autologous Fibroblast Transplantation along with Low-Level Laser Therapy.

Low Level Laser Therapy (LLLT) has been used as an effective therapeutic modality since the mid sixties. Although there are several clinical studies using LLLT in wound healing especially diabetic, pressure and venous ulcers, but there are rare reports of using this technique in burn ulcers. In this study for the first time we used LLLT along with antilogous fibroblast skin transplantation to treat grade 3 burn ulcers in diabetic patients.

Ten diabetic patients with grade 3 burn ulcer, candidate for skin graft surgery entered the study. 1 Cm2 was biopsied using punch. Fibroblasts were extracted and cultured in-vitro. Patients were treated using LLLT in 3-4 weeks that took time that fibroblast cultures become ready to use. Laser irradiation was done using red red light, 650 nm, 150 mW, 1 J/ cm2  for the bed of the ulcer and infra red light 808 nm, 200 mW, 6 J/ cm2 for the margins every other day for 10 sessions. Then a thin layer of fibroblast suspension was applied to the base of ulcer using sterile sampler and its surface was covered by Vaseline gauze.

All patients healed completely in 10-12 weeks. No adverse effects were reported.

We conclude that this method can be used as an effective method for treating large wounds especially in complicated patients including diabetics.

A deep burn ulcer, especially in areas with sensory-motor dysfunction, is hard to cure.Case Report: The patient was a 20-year-old paraplegic girl with a grade 3 necrotic burn ulcer for 3 weeks. We used a fractional Co2 laser along with chemical debridement with trichloroacetic acid (TCA 80%) and low-level laser therapy (LLLT) with a 808 nm infrared laser, 6 J/cm2 for the necrotic area, and a 650 nm red laser, 2 J/cm2 for the open wound area. Complete healing occurred after 25 sessions without surgery.

Laser debridement along with LLLT and TCA administration may be useful to treat necrotic ulcers without surgery.Keywords: Co2 Laser debridement; Trichloroacetic Acid; Low-level laser Therapy; Wound healing.

The objective of this study was to compare the effectiveness of Emotion-Focused Cognitive-Behavioral Therapy (ECBT) and Mindfulness-Based Cognitive Therapy (MBCT) on the C-reactive protein (CRP) level in patients with psoriasis.

This research is a quasi-experimental study with a pretest-posttest design, a follow-up period, and control and intervention groups. The statistical population included all patients with psoriasis visiting Skin and Stem Cell Research Center (SSRC), Tehran University of Medical Sciences (TUMS). In this study, 30 patients were selected as the sample and were randomly assigned to the control group (5 males and 5 females), the first intervention group (5 males and 5 females), and the second intervention group (5 males and 5 females). Patients in the first and second intervention groups participated in 8 sessions of ECBT and MBCT, respectively, and subjects in the control group received no intervention and were placed on the waiting list. The pretest, posttest, and follow-up CRP level were measured using laboratory kits. The data were analyzed using the repeated measures ANOVA through SPSS v22.

The results showed that both ECBT and MBCT reduced the CRP level equally. The same results were also obtained during the follow-up period.

The study findings suggested that both ECBT and MBCT can be effective treatments for physical, psychological, and biological problems caused by psoriasis.

Skin damage is one of the most common injuries that occur daily for millions around the world. Considering this high prevalence, developing new methods of cells and stem cell administration for healing injured skin is necessary. We discuss different new methods of stem cell and cell administration for repairing injured skin, such as skin stem cell gun, scaffolds, hydrogels, and autologous keratinocytes sheets.

  Many approaches are available in injured skin healing, such as traditional therapy, transplanting, cell therapy, and stem cell therapy. Skin grafting is still a standard solution to treat patients; however, there are a lot of limitations in this procedure, for example, patients with severe burns have an insufficient skin area for grafting, which is a painful procedure.

Although cell therapy approaches dissolve scarring and reduce healing time, considering its high cost, they are not used in everyday society. This article discusses different regenerative dermatology products and compares them with each other.

Herein, we introduced several examples of skin cell products in the pharmaceutics market. Although these products are not well known and even some of them didn’t reach clinical trials, comparing different kinds of skin engineered products reveals appropriate guidelines for their use and entrance to dermatology market

The current study aimed at evaluating the role of apoptosis in hair and skin conditions.

  In the current study, the role of apoptosis in the occurrence or treatment of the apoptosis-associated disease was investigated. Likewise, the mechanism of apoptosis pathways occurring in the skin and hair cells was also reviewed.

Melanocyte and follicular epithelial cells are the most sensitive cells to apoptosis, whereas dermal papilla cells, fibroblasts, and some of keratinocytes are the most resistance cells to apoptosis. According to the current review study, novel drugs can be designed based on stimulation or inhibition of the apoptosis such as BCL2, p53 and caspase inhibitors.

Pemphigus vulgaris is an autoimmune disorder, which appears as a mucosal intracutaneous blister. The disease prevalence is different in various countries and regions. Therefore, identification of the prevalence and characteristics of patients with this disease in different climates can be useful in initiating early treatment and management, and reducing the mortality. So, the aim of this study was to evaluate the epidemiology of Pemphigus vulgaris in Isfahan City, Iran during a ten-year period. In this descriptive retrospective study, 114 patients with Pemphigus vulgaris who were admitted in Alzahra hospital in Isfahan during the years of 2006-2017 were studied. Demographic, clinical, and paraclinical data were recorded, and the results were reported using SPSS software. Findings: Of 114 patients, 78 (68.4%) were women and (31.6%) 36 were men, with a mean age of 49.15 ± 12.55 years. Type of the lesion was mucosal and vegetative in 14.9%, cutaneous in 24.4%, and both mucosal and cutaneous in 60.5%. In addition, the prevalence of this disease in the spring and summer was 31.6% and 27.2%, respectively. Most patients were treated with prednisolone and Azaram drugs, which were associated with complications in 17.8%; but eventually all patients recovered and no mortality occurred during this assessment. According to the results of the study, the prevalence of this disease in women was more than men, and most of them appeared in spring and summer as cutaneous-mucosal manifestations. Therefore, it seems that more research is needed on the prevalence of this disease and its manifestations, the season of more prevalence, and successful treatments.