فهرست مطالب rohangiz naderi

Indoor air pollution is one of the most important environmental subjects in the world with serious side effects on human health. We report the result of three replications and a factorial design experiment that assesses the effect of plants (Epipremnum aureum and Dracaena trifasciata ) on the removal of indoor air pollution. To gain the objects, potted plants were placed individually in 125 liters airtight glass containers (chamber) which had a small valve to inject and sucking airs. Each plant was exposure to, benzene 25 and 50 µl/l, acetone 25 and 50 µl/l, ethanol 50 µl/l and methanol 50 µl/l during a day (24 hours). According to the biochemical examination electrolyte leakage (%) of pothos was decreased especially in acetone 25 µl/l versus on treated sansevieria increased. Total chlorophyll content of sansevieria in all treatments was increased, while it was decreased when pothos exposure to benzene 25, benzene 50 µl/l and methanol 50 µl/l catalase activity and superoxide dismutase showed converse results. In both plant species catalase activity was decreased especially in acetone 50, however superoxide dismutase activity was increased in all treatments and in sansevieria exposure to benzene 50 µl/l more than others. There was no significant change in proline content of pothos, although there was significant increase in acetone 50 µl/l and ethanol 50 µl/l of sansevieria. The results indicate when plants exposure to the benzene, tissue cells damaged more than other treatments while, acetone had least amount of tissue cell damage. According to this paper results, both plant species have the potential of indoor air pollution phytoremediation but, pothos had higher adaptability in pollutant indoor area.

Drought stress is one of limitation factors in landscape expantion. In order to investigate response of four wild roses species (Rosa canina, R. beggeriana, R. orientalis & R. persica) to water deficit, a factorial experiment was carried out in a completely randomized block design with two factors Rosa species and water deficit at three levels (control (-0.03), -0.8 (moderate) & -1.5 (Severe) MPa). One year old plant obtained from mentioned rose sucker were subjected to water deficit treatment. Based on the results, leaf mass area (LMA) was different between species and water deficit had no effect on that. Water deficit caused decreased relative leaf water content (RWC), performance index (PI), chlorophyll content and increased electric leakage (EL) and malondialdehyde (MDA) in R. persica. In R. canina RWC, PI and free radical scavenging capacity (DPPH) decreased and EL and MDA increased. In R. beggeriana EL and DPPH increased and in other characteristics difference was invisible. In R. orientalis water deficit had no dramatic effect on traits. Based on the results R. orientalis and then R. beggeriana are more tolerable to drought stress.

The cyclamen (Myrsinascea) as the flowers growing in some temperate regions, are of high ecological and botanical importance and endangered as well. Therefore, to provide an opportunity to identify and protect its species, Iranian native cyclamen was studied. Plants were collected (in winter and spring) from three northern provinces of Iran, i.e., Guilan, Mazandaran, and Golestan, and their morphological traits (14 quantitative and 10 qualitative traits) were assessed. Based on the analysis of variance, there were significant differences in some recorded traits, e.g., leaf number, flower number, flower color, and flower and leaf shape, which would allow selecting plants with more optimal traits. The cluster analysis divided the plants into three groups. Accordingly, the plants collected from the Shadan region in Golestan province and those collected from the Sinava and Shahsheshmeh regions in Chalus County, Mazandaran province differed from the plants collected from the other regions. The plants were collected from the Abpari and Lavij regions in Nur County, the Pasand region in Behshahr County, the Qarnabad region in Gorgan County, and the Deylaman region in Siahkal County were placed in the same group due to their similar morphology although they had been collected from three different provinces. The first group included the cyclamens of the Qarnabad region in Gorgan County, the Lavij region in Nur County, the Deylaman region in Siahkal County, the Pasand region in Behshahr County, and the Abpari region in Nur County. These plants had lowly toothed leaf margins, long petals, and few seeds. The second group included the cyclamens of the Shadan region in Kordkuy County. These plants had longer and heavier hypocotyls, larger and wider leaves, and smaller flowers. They showed the highest number of seeds, hypocotyl diameter, length, and weight, flower number, leaf length and width, leaf area, petiole and peduncle length, and peduncle diameter. The third group included the cyclamens of the Sinava and Shahsheshmeh regions in Chalus County. They had the highest petal width but the lowest peduncle diameter, leaf length, width, and area, flower and leaf number, and hypocotyl weight, length, and diameter.  The cyclamens native to Iran are highly diverse in morphological traits and they need to be further investigated by cytogenetic, phytochemical, and molecular methods.

Drought stress is one of the most important environmental factors, which limit the growth of plants. By the end of the 21st century, the incidence of drought stress is expected to increase because of the global warming phenomenon. As a consequence, trees growth and viability in the forests and urban greenspace will reduce. Thus, selection of plants that are more tolerant to severe drought stress and are able to cope with such environmental conditions needs to be considered in future silvicultural strategies. This study was carried out to identify candidate drought-tolerance proteins in Maclura pomifera. Therefore, we aimed to explore the performance of Maclura pomifera under a severe drought stress and analyse the proteome changes of Maclura pomifera leaf in response to drought.

The experiment was carried out on 4-year-old Maclura pomifera saplings genotypes cultivated on a flat field in the Botanical Garden of University of Tehran. Saplings were exposed to irrigation regimes of 100% and 25% field capacity in a completely randomized design. Leaf samples were collected and were frozen immediately in liquid nitrogen and then stored at −80◦C to be used for further analyses. Experiments were performed using the gradient pH 3-10 NL IPG strips for the isoelectric focusing. IEF was carried out using the PROTEAN IEF. Strips were then equilibrated first for 15 min in reducing solution and then 15 min in alkylating solution. Equilibrated IPG strips were then placed and fixed using hot agarose on the top of home-made 12 % SDS- polyacrylamide gels. Separation of proteins in the second dimension was carried out in Protean XL cell. The protein spots were visualized by staining with BioSafe Coomassie gel stains following manufacturer’s instructions.

After doing two-dimensional gel electrophoresis, 25 protein spots that had displayed significant protein level changes were identified. Differentially expressed, proteins were divided in three groups. The first group included stress and defense proteins such as lipoxygenase, two types of heat shock protein, Allergen, Convicilin and legumin A2 precursorm; the second group included oxidative stress proteins such as Catalase, Chloroplast stromal ascorbate Peroxidase, Cytosolic ascorbate peroxidase, Iron superoxide dismutase and Manganese superoxide dismutase. Thethird group included energy and metabolism proteins such as Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, Ribulose -1,5- bisphosphate carboxylase /oxygenase small subuni, Translation elongation factor, Aldolase, Hydroxy-acid oxidase, Isopentenyl diphosphate isomerase, Dihydrolipoamide dehydrogenase and glyoxalase. The present results indicate that most proteins have been identified and their changes caused an increase in tolerance and adaptation of Maclura pomifera to drought stress. Also, our data suggest that drought tolerance of M. pomifera might be correlated with diminishing oxidative damage by activation of the antioxidant systems.

The present results indicate that most proteins have been identified and their changes caused an increase in tolerance and adaptation of Maclura pomifera to drought stress. Also, our data suggest that drought tolerance of M. pomifera might be correlated with diminishing oxidative damage by activation of the antioxidant systems.

To evaluate the effect of some air pollutants on photosynthetic pigments and stress resistance parameters in Sansevieria trifasciata L., a completely randomized experiment with 7 treatments including control, benzene 50 and 25 ml.l-1 , acetone 50 and 25 ml.l -1 , ethanol 50 ml.l-1 and methanol 50 ml.l-1 with three replications. To apply the treatments, glass chambers with closed environments and same volume and area were prepared, each glass chamber containing valves for injection or air suction. One plant was placed in each chamber and the plant volume was measured and then certain concentrations of contaminants were injected into the chambers. The results showed that the evaluated air pollutants had a negative effect on the studied characteristics compared to the control, but acetone 50 ml.l-1 caused the most severe stress in censorship and stimulated the production of reactive oxygen species, thereby increasing proline production and increasing the plant's defense system increased protein levels. The photosynthetic activity of the Sansevieria plant also increased under the influence air pollutants. Accordingly, it seems that Sansevieria plant has a good resistance to the dominant air pollutants in the construction space of hospitals, laboratories, etc., which is recommended for these places.

Selenium (Se) is an essential microelement for humans and a beneficial element for plants. Recently, biofortification with Se has emerged as a key practice to increase plant quality. The aim of present study was to investigate the postharvest responses of Pelargonium peltatum L. to the application of Se in soil. The Se was used as sodium selenite (Na2SeO4) in soil application of 20, 40, and 60 µM L-1 distilled water. The results showed that flower weight, relative water content (RWC), anthocyanin, and vitamin C decreased over the storage time. Malondialdehyde (MAD) increased by progressing the time of storage. Total phenolic content (TPC) and total flavonoid content (TFC) were significantly enhanced at early days of storage and then decreased up to the end of storage on days 4 and 6. Se significantly improved flower weight, RWC, anthocyanin, vitamin C, TPC, and TFC entire the storage. Although, Se was effective in enhancing the quality of P. peltatum L., there was no significant difference between 40 and 60 µM Se in all traits. Therefore, this experiment suggests using 40 µM Se to alleviate adverse effects induced by postharvest time growth on productivity of P. peltatum L.

Benefits of cut style method and ovary slice culture for overcoming pre-and post-zygotic barriers in lily crossing were studied. Three Asiatic hybrid cultivars (Tressor, Pirandello and Ceb duzzel) along with four cultivars of Oriental section (Rialto, Sorbonne, Paradero and Manissa) were used as maternal plants. Lilium ledebourii has selected as pollen donor plant to cross with maternal plants using stigma pollination or cut style pollination methods. Harvesting of produced capsules for ovary slice culture was done after 10, 20 and 40 days. Ovary expansion index, number of produced capsules, capsule weight, and number of cultured slices, number of produced seeds, seed weight and number of germinated seeds were recorded. Results indicated that cultivar had significant influence on all recorded parameters. The number of seeds was significantly affected by capsule harvesting time (p<0.05). In cut style experiment in crosses between Lilium ledebourii and Mannisa, Tressor and Sorbonne 172, 80 and 60 seeds were produced respectively. The rate of seed germination for Mannisa by L. ledebourii was 1.1 percent. In stigma pollination experiment capsules were expanded but none of them produced viable seeds. Totally, in this experiment, use of CSM pollination method was successful for by passing pre-zygotic barriers in lily interspecific crossing.

The main object of this study is to investigate the effect of plant growth regulators on the prolongation of vase life of two kinds of cut carnation under normal and ethylene. Carnation is one of the most popular flowers that has a short life due to its sensitivity to ethylene. Also, the effect of plant growth regulators on several physiological traits under ethylene stress and normal conditions was studied. The effects of short-term treatment with benzyl adenine, salicylic acid, and methyl jasmonate on two types of cut carnations (Dianthus caryophyllus L.), one-headed standard cut carnation and multi- flower mini cut carnation, under exposure of ethylene condition were investigated. Benzyl adenine 100 µM short-term treatment had the most effect on total chlorophyll content. Salicylic acid 100 µM treatment enhanced the soluble carbohydrates, and also prolonged the cut carnation vase life. Methyl jasmonate 400 µM treating elevated the catalase and peroxidase enzymes activity and increased the proline content in cut carnations. All considered traits were reduced by exogenous ethylene. None of short-term treatments could repel the inappropriate exogenous ethylene effects. One-headed standard carnations were also tolerant of ethylene more than multi- flower mini carnations.

Alstroemeria is a cut-flower that has recently become increasingly popular because of its beautiful flowers of various colors، and high yield. Leaf yellowing is considered as a primary index of Alstroemeria flower deterioration. In order to study how to alleviate this disorder and improve the flower،s longevity as well as its other quality traits، a factorial experiment was conducted on the «Napoli» cultivar of Alstroemeria cut-flowers. Throughout the experiment، different levels of ethanol (0، 2، 4، 6%) and of GA3 (0، 50، 100، 150 mg/liter) along with 4% sucrose، were applied in preservative solutions in either one of the two forms of pulse or continuous application. The evaluated traits were: flower longevity، chlorophyl content، flower fresh weight، solution uptake، relative water content، flower diameter، as well as ion leakage. Based on the obtained results، the most leaf chlorophyll content was found in cut-flowers treated with 100 mg/L GA3 in either one of the pulse or continuous methods. But، the continuous method proved to have better effects on flower vase life، solution uptake، flower fresh weight and as well on the diameter. Also، application of 2% ethanol along with either 100 or 150 mg/L of GA3 was found out to bring about the most increased flower longevity. Application of higher concentrations of ethanol in this treatment caused a decrease in the flower vase life.